负载mRNA脂质纳米颗粒的聚己内酯微胶囊的精密微流控制备用于缓释递送
Precise microfluidic engineering of mRNA lipid nanoparticle-loaded polycaprolactone microcapsules for sustained delivery.
摘要(中文译文)
mRNA疗法在治疗多种疾病方面具有前景，但其临床应用面临重大挑战，包括快速降解和需要频繁注射。脂质纳米颗粒（LNPs）是目前临床上最先进的mRNA递送系统，但其半衰期短和注射后突释等问题阻碍了其有效性。在本研究中，我们开发了一种基于微胶囊的系统，利用聚己内酯（PCL）包封mRNA LNPs，旨在提高其稳定性、延长滞留时间并实现缓释。微胶囊采用定制微流控方法制备，获得了尺寸和壳厚度精确可控的均匀微胶囊。释放研究表明，这些微胶囊为mRNA LNPs提供了有效保护，并在较长时间内控制其释放。研究证明，载有mRNA LNP的微胶囊显著延长了mRNA在小鼠体内的滞留时间，单次注射后mRNA表达维持长达8天。这些发现凸显了该基于微胶囊的递送系统在长效mRNA递送方面的潜力，可提高治疗效果和患者依从性。
Abstract
mRNA therapy holds promise for treating a variety of diseases, but its clinical application faces significant challenges, including rapid degradation and the need for frequent injections. Lipid nanoparticles (LNPs) are currently the clinically advanced delivery system for mRNA, yet issues such as short half-life and burst release after injection hinder their effectiveness. In this study, we developed a microcapsule-based system using polycaprolactone (PCL) to encapsulate mRNA LNPs, aiming to improve their stability, prolong retention, and enable sustained release. Microcapsules were fabricated using a customized microfluidic approach, resulting in uniform microcapsules with precise control over size and shell thickness. release studies showed that these microcapsules provided effective protection for mRNA LNPs and controlled their release over extended periods. studies demonstrated that mRNA LNP-loaded microcapsules significantly extended the retention time of mRNA in mice, maintaining mRNA expression for up to 8 days following a single injection. These findings highlight the potential of this microcapsule-based delivery system for long-lasting mRNA delivery, improving both therapeutic efficacy and patient compliance.
如何引用
AMA
Hao Bai, Baiqiu Chen, Ziyue Wang, Hongquan Wang, Zhenghao Hu, Yiqing Chen, et al. Precise microfluidic engineering of mRNA lipid nanoparticle-loaded polycaprolactone microcapsules for sustained delivery.. Journal of materials chemistry. B. 2026; doi:10.1039/d6tb00833j.
APA
Hao Bai, Baiqiu Chen, Ziyue Wang, Hongquan Wang, Zhenghao Hu, Yiqing Chen, et al (2026). Precise microfluidic engineering of mRNA lipid nanoparticle-loaded polycaprolactone microcapsules for sustained delivery.. Journal of materials chemistry. B. https://doi.org/10.1039/d6tb00833j
GB/T 7714
Hao Bai, Baiqiu Chen, Ziyue Wang, Hongquan Wang, Zhenghao Hu, Yiqing Chen, et al. Precise microfluidic engineering of mRNA lipid nanoparticle-loaded polycaprolactone microcapsules for sustained delivery.[J]. Journal of materials chemistry. B, 2026 doi:10.1039/d6tb00833j.
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