基于铁MOF的荧光和比色双读出纳米探针，用于灵敏检测α-硫霉素
Fe-MOF-based fluorescent and colorimetric dual-readout nanoprobe for the sensitive detection of α-lipomycin.
摘要(中文译文)
盐敏感性高血压构成了重大的全球健康负担，但其潜在的致病机制仍未完全阐明。近期证据表明，微生物代谢产物α-脂霉素通过选择性抑制内皮TRPV4通道导致血管功能障碍，从而损害血管舒张并促进盐敏感性高血压的发生发展。因此，对α-脂霉素进行高灵敏度和高选择性的检测对于早期风险评估和干预至关重要。本研究采用溶剂热法，以TCPP为有机配体、Fe(III)为金属中心，制备了Fe-MOF纳米棒，并将其用于α-脂霉素的检测。α-脂霉素与Fe-MOFs中Fe(III)中心之间的特异性主客体效应可选择性地破坏Fe-MOFs内的配体-金属电荷转移，触发645 nm处的同步荧光发射以及棕色显色现象。因此，Fe-MOF纳米棒可作为荧光和比色纳米探针用于α-脂霉素的双模式检测。该传感器表现出0.05-10 µM的宽线性响应范围，以及23.83 nM（荧光法）和409.80 nM（比色法）的超低检测限。此外，Fe-MOF纳米探针对α-脂霉素的双信号响应进一步赋予了其优异的抗干扰选择性和高重复性（RSD < 5.64%）。值得注意的是，使用自来水的实际验证显示出93.47%至109.35%的可接受回收率。重要的是，临床血清分析表明高血压患者中α-脂霉素水平升高，验证了Fe-MOF纳米探针在早期致病检测和治疗监测方面的强大实用性。这些发现凸显了Fe-MOF纳米探针作为一种双模式光学平台用于选择性和可靠地检测α-脂霉素的潜力。其可现场部署的设计克服了传统方法（如质谱法）在临床应用中的局限性。它也为盐敏感性高血压的致病机制研究、分型、早期预警和预防等应用提供了一种有前景的方法。
Abstract
Salt-sensitive hypertension represents a significant global health burden, yet its underlying pathogenic mechanisms remain incompletely understood. Recent evidence indicates that the microbial metabolite α-lipomycin contributes to vascular dysfunction by selectively suppressing the endothelial TRPV4 channels, thereby impairing vasodilation and promoting the development of salt-sensitive hypertension. Consequently, the highly sensitive and selective detection of α-lipomycin is of critical importance for early risk assessment and intervention. Herein, Fe-MOF nanorods were prepared using TCPP as the organic ligand and Fe(III) as the metal center a solvothermal method and used for α-lipomycin detection. Specific host-guest effects between α-lipomycin and the Fe(III) centers in Fe-MOFs selectively disrupt the ligand-to-metal charge transfer within Fe-MOFs, triggering simultaneous fluorescence emission at 645 nm and a brown chromogenic appearance. Consequently, Fe-MOF nanorods serve as a fluorescent and colorimetric nanoprobe for the dual-mode detection of α-lipomycin. The sensor demonstrated a broad linear response range of 0.05-10 µM and ultralow detection limits of 23.83 nM (fluorescence) and 409.80 nM (colorimetry). Furthermore, the dual-signal response of the Fe-MOF nanoprobe toward α-lipomycin further confers exceptional selectivity against interferents and high repeatability (RSD < 5.64%). Notably, practical validation using tap water shows acceptable recovery rates of 93.47% to 109.35%. Importantly, clinical serum analyses demonstrate elevated α-lipomycin levels in hypertensive patients, validating the strong practicability of the Fe-MOF nanoprobe for early pathogenesis detection and therapeutic monitoring. These findings highlight the Fe-MOF nanoprobe as a dual-mode optical platform for the selective and reliable α-lipomycin detection. Its field-deployable design overcomes the clinical limitations in the utility of conventional methods, such as mass spectrometry. It also offers a promising approach for applications in pathogenesis research, typing, early warning, and prevention of salt-sensitive hypertension.
如何引用
AMA
Xianfeng Wang, Genchang Chen, Gongle Shen, Zhenping Liu, Fengwu Lin, Lei Shen, et al. Fe-MOF-based fluorescent and colorimetric dual-readout nanoprobe for the sensitive detection of α-lipomycin.. Journal of materials chemistry. B. 2026; doi:10.1039/d6tb00290k.
APA
Xianfeng Wang, Genchang Chen, Gongle Shen, Zhenping Liu, Fengwu Lin, Lei Shen, et al (2026). Fe-MOF-based fluorescent and colorimetric dual-readout nanoprobe for the sensitive detection of α-lipomycin.. Journal of materials chemistry. B. https://doi.org/10.1039/d6tb00290k
GB/T 7714
Xianfeng Wang, Genchang Chen, Gongle Shen, Zhenping Liu, Fengwu Lin, Lei Shen, et al. Fe-MOF-based fluorescent and colorimetric dual-readout nanoprobe for the sensitive detection of α-lipomycin.[J]. Journal of materials chemistry. B, 2026 doi:10.1039/d6tb00290k.
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