用于单细胞封装的微流体液滴内的水凝胶光聚合
Hydrogel photopolymerization within microfluidic droplets for single cell encapsulation.
摘要(中文译文)
单细胞水平上特定基因表达模式的分子研究揭示了大量细胞群体的异质性，有助于理解隐藏的相互作用调控通路。然而，数十年来，传统宏观工具与单细胞尺寸之间的长度尺度差异一直制约着这种理解。此外，这些工具无法在合理较短的时间内高效操控数百万个单细胞。因此，上述局限性相关问题对单细胞包封及后续分析构成了挑战。本综述重点介绍了液滴微流控技术的最新进展，这些技术实现了载细胞皮升级液滴的高通量制备，随后可通过光聚合将液滴转化为微凝胶，用于进一步分析评估或自下而上的组织工程实践。本文介绍了为单细胞筛选、分析及组织工程支架组装而开发的新型微流控液滴技术。同时，在实现单细胞操控的背景下，讨论了微流控液滴微加工过程中遇到的限制与挑战。最后，介绍了一种新兴替代材料，该材料在微小型水凝胶中的单细胞包封方面具有巨大潜力，其具有生物相容性聚合动力学和细胞相容性，可作为细胞载体。
Abstract
Molecular investigation of specific gene expression patterns at the single-cell level reveals the heterogeneity of bulk cell populations, facilitating the understanding of interactive regulatory pathways that are hidden. However, this understanding has been constrained for decades by the length scale differences between traditional macroscopic tools and the size of a single cell. Additionally, these tools are inadequate in efficiently manipulating millions of individual cells within a reasonably short time period. Therefore, issues associated with the above limitations represent a challenge for single-cell encapsulation and subsequent analysis. This review highlights recent developments in droplet-microfluidic techniques that have enabled the high throughput fabrication of cell-laden picoliter droplets, which can be subsequently photopolymerized into microgels for further analytical assessment or bottom-up tissue engineering practices. Novel microfluidic droplet techniques developed for single-cell screening, analysis, and scaffold assembly for tissue engineering are introduced. Meanwhile, the constraints and challenges encountered in microfluidic droplet miniaturization processing are discussed in the context of enabling single-cell manipulation. In the end, an emerging alternative material, which holds great promise for single-cell encapsulation in miniaturized hydrogels, is introduced as a cell carrier with biocompatible polymerization kinetics and cytocompatibility.
如何引用
AMA
Shiqi Lei, Xingdan Wang, Yan Liu, Yuheng Du, Zhihuan Li, Yajuan Guo, et al. Hydrogel photopolymerization within microfluidic droplets for single cell encapsulation.. Journal of materials chemistry. B. 2026; doi:10.1039/d6tb00258g.
APA
Shiqi Lei, Xingdan Wang, Yan Liu, Yuheng Du, Zhihuan Li, Yajuan Guo, et al (2026). Hydrogel photopolymerization within microfluidic droplets for single cell encapsulation.. Journal of materials chemistry. B. https://doi.org/10.1039/d6tb00258g
GB/T 7714
Shiqi Lei, Xingdan Wang, Yan Liu, Yuheng Du, Zhihuan Li, Yajuan Guo, et al. Hydrogel photopolymerization within microfluidic droplets for single cell encapsulation.[J]. Journal of materials chemistry. B, 2026 doi:10.1039/d6tb00258g.
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