{“_”：“从.中提取的水和碱多糖的结构特征和肠道微生物群介导的免疫调节机制”，“i”：[“Tuber indicum”]}
{"_":"Structural Characteristics and Gut Microbiota-Mediated Immunomodulatory Mechanisms of Water- and Alkali-Extracted Polysaccharides from .","i":["Tuber indicum"]}
摘要(中文译文)
是一种稀有的食药两用外生菌根真菌，其多糖组分因具有强大的免疫调节潜力而受到广泛研究关注。为系统表征水提多糖（TIWP）和碱提多糖（TIAP）的结构特征及肠道菌群介导的免疫调节机制，本研究制备了两种多糖组分并进行了全面的结构表征。TIWP的最大分子量达到2.65×10 Da，其主要糖苷键类型为→3)-Glcp-(1→、→2,4)-Glcp-(1→和→3,6)-Glcp-(1→。TIAP具有更高的分子量，高达3.87×10 Da，其主要糖苷键为→3,6)-Glcp-(1→、→4)-Glcp-(1→和→2,4)-Glcp-(1→。体内生物活性评价结果表明，TIWP和TIAP均能显著恢复外周血和脾脏中CD3 T淋巴细胞和CD19 B淋巴细胞亚群的比例，并减轻脾脏和结肠组织的病理损伤。两种多糖对肠道菌群表现出不同的调控模式：TIWP显著富集了和属，而TIAP则提高了和的相对丰度。非靶向代谢组学结合KEGG通路富集分析揭示，TIWP和TIAP激活了泛酸和辅酶A生物合成通路以及亚油酸代谢通路。综上所述，TIWP和TIAP因其结构差异，通过不同的“肠道菌群-代谢物-免疫”调控网络缓解CTX触发的免疫抑制。本研究为开发具有免疫调节功能的靶向肠道微生态功能性食品提供了理论依据。
Abstract
is a rare edible and medicinal ectomycorrhizal fungus, and the polysaccharide fractions have attracted extensive research attention owing to their potent immunomodulatory potential. To systematically characterize the structural features and gut microbiota-mediated immunoregulatory mechanisms of water-extracted polysaccharide (TIWP) and alkali-extracted polysaccharide (TIAP) from , two polysaccharide fractions were prepared and comprehensively structurally characterized in this study. The maximum molecular weight of TIWP reached 2.65 × 10 Da, which was dominated by glycosidic linkages of →3)-Glcp-(1→, →2,4)-Glcp-(1→ and →3,6)-Glcp-(1→. TIAP possessed a higher molecular weight up to 3.87 × 10 Da, with predominant glycosidic bonds of →3,6)-Glcp-(1→, →4)-Glcp-(1→ and →2,4)-Glcp-(1→. In vivo bioactivity evaluation results demonstrated that both TIWP and TIAP significantly restored the proportions of CD3 T and CD19 B lymphocyte subsets in peripheral blood and spleen, and alleviated pathological damage in splenic and colonic tissues. Distinct regulatory patterns of gut microbiota were observed between the two polysaccharides: TIWP markedly enriched the genera and , whereas TIAP elevated the relative abundances of and . Untargeted metabolomics combined with KEGG pathway enrichment analysis revealed that TIWP and TIAP activated the pantothenic acid and coenzyme A biosynthesis pathway and the linoleic acid metabolism pathway. Collectively, TIWP and TIAP alleviated CTX-triggered immunosuppression through distinct "gut microbiota-metabolite-immunity" regulatory networks due to their structural disparities. This study provides a theoretical basis for the development of gut microecology-targeted functional foods with immunomodulatory functions.
如何引用
AMA
Hongfei Ji, Mei Li, Decheng Mao, Yujie Chen, Bing Han, Yanli Zheng, et al. {"_":"Structural Characteristics and Gut Microbiota-Mediated Immunomodulatory Mechanisms of Water- and Alkali-Extracted Polysaccharides from .","i":["Tuber indicum"]}. Nutrients. 2026; doi:10.3390/nu18132202.
APA
Hongfei Ji, Mei Li, Decheng Mao, Yujie Chen, Bing Han, Yanli Zheng, et al (2026). {"_":"Structural Characteristics and Gut Microbiota-Mediated Immunomodulatory Mechanisms of Water- and Alkali-Extracted Polysaccharides from .","i":["Tuber indicum"]}. Nutrients. https://doi.org/10.3390/nu18132202
GB/T 7714
Hongfei Ji, Mei Li, Decheng Mao, Yujie Chen, Bing Han, Yanli Zheng, et al. {"_":"Structural Characteristics and Gut Microbiota-Mediated Immunomodulatory Mechanisms of Water- and Alkali-Extracted Polysaccharides from .","i":["Tuber indicum"]}[J]. Nutrients, 2026 doi:10.3390/nu18132202.
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