新分离菌株Pseudochrobactrum asaccharolyticum TY12对头孢克肟的高效生物降解及其降解机理的研究
{"_":"Efficient Biodegradation of Cefixime by the Newly Isolated Strain TY12 and Insights into Its Degradation Mechanism.","i":["Pseudochrobactrum asaccharolyticum"]}
摘要(中文译文)
农业土壤、食品系统和水环境中的头孢菌素残留对食品安全、公众健康和生态环境构成严重威胁。尽管生物降解是一种有前景的修复策略，但大多数头孢菌素的降解机制仍不明确。本研究分离出TY12，并证明其具有降解多种头孢菌素的广谱能力。值得注意的是，菌株TY12对头孢克肟（CFX）的降解效率最高，在12小时内对100 mg/L CFX的去除率达到93.87%。通过高分辨率质谱鉴定出CFX的10种降解产物，并据此提出了降解途径。值得注意的是，这些产物的抗菌活性显著减弱。转录组分析和异源表达鉴定出β-内酰胺酶TY3865为关键酶。此外，分子对接和定点突变揭示了对其催化活性至关重要的残基。这些发现为抗生素生物降解提供了机制性见解，并证明了TY12在处理头孢菌素污染废水方面的潜力。
Abstract
Cephalosporin residues in agricultural soils, food systems, and aquatic environments pose severe threats to food safety, public health, and the ecological environment. While biodegradation represents a promising remediation strategy, the degradation mechanisms for most cephalosporins remain elusive. In this study, TY12 was isolated and demonstrated a broad-spectrum ability to degrade multiple cephalosporins. Notably, strain TY12 exhibited the highest cefixime (CFX) degradation efficiency, achieving a 93.87% removal of 100 mg/L CFX within 12 h. Ten degradation products of CFX were identified via high-resolution mass spectrometry, leading to a proposed degradation pathway. Notably, these products exhibited significantly attenuated antibacterial activity. Transcriptomic analysis and heterologous expression identified β-lactamase TY3865 as the key enzyme. Furthermore, molecular docking and site-directed mutagenesis revealed the residues critical for its catalytic activity. These findings provide mechanistic insights into antibiotic biodegradation and demonstrate the potential of TY12 for treating cephalosporin-contaminated wastewater.
如何引用
AMA
Boxiang Kou, Zexun Wang, Yujia Wang, Meng Shen, Zhaoyi Zheng, Yecheng Gao, et al. {"_":"Efficient Biodegradation of Cefixime by the Newly Isolated Strain TY12 and Insights into Its Degradation Mechanism.","i":["Pseudochrobactrum asaccharolyticum"]}. Journal of agricultural and food chemistry. 2026; doi:10.1021/acs.jafc.6c04944.
APA
Boxiang Kou, Zexun Wang, Yujia Wang, Meng Shen, Zhaoyi Zheng, Yecheng Gao, et al (2026). {"_":"Efficient Biodegradation of Cefixime by the Newly Isolated Strain TY12 and Insights into Its Degradation Mechanism.","i":["Pseudochrobactrum asaccharolyticum"]}. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.6c04944
GB/T 7714
Boxiang Kou, Zexun Wang, Yujia Wang, Meng Shen, Zhaoyi Zheng, Yecheng Gao, et al. {"_":"Efficient Biodegradation of Cefixime by the Newly Isolated Strain TY12 and Insights into Its Degradation Mechanism.","i":["Pseudochrobactrum asaccharolyticum"]}[J]. Journal of agricultural and food chemistry, 2026 doi:10.1021/acs.jafc.6c04944.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出