用于同时检测食品和环境样品中硝碘酚腈和氯羟柳胺的多重免疫层析检测法
A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and Environmental Samples.
摘要(中文译文)
广泛使用的驱虫药物硝碘酚腈（NIT）和羟氯柳胺（OXY）对生态环境和人类健康构成潜在风险，因此需要一种灵敏、快速的检测方法。本研究设计并合成了两种分别靶向OXY和NIT的新型半抗原，并通过计算机模拟评估了其结构有效性。通过动物免疫和细胞融合，获得了单克隆抗体（OXY-1A4和NIT-2F12），其半数抑制浓度分别为1.13 ng/mL和0.23 ng/mL。在此基础上，建立了一种基于金纳米颗粒的多重免疫层析 assay（multi-AuNPs-ICA），用于同时检测废水、牛奶和牛肉中的OXY和NIT。目视法和计算法的检测限分别为2～20 ng/mL和0.37～7.16 ng/mL。废水、牛奶和牛肉样品中的加标回收率分别为102.6%～110.5%、90.4%～115.6%和86.9%～117.7%。这些结果与仪器方法高度一致，证实了multi-AuNPs-ICA的可靠性及其在现场快速筛查方面的显著潜力。
Abstract
The widely used deworming drugs nitroxynil (NIT) and oxyclozanide (OXY) pose potential risks to the ecological environment and human health, necessitating a sensitive and rapid detection method. In this study, two novel haptens targeting OXY and NIT, respectively, were designed and synthesized, with their structural validity evaluated by computer simulation. Through animal immunization and cell fusion, monoclonal antibodies (OXY-1A4 and NIT-2F12) were obtained, with half-maximal inhibitory concentrations of 1.13 ng/mL and 0.23 ng/mL, respectively. Based on this, a multiplex gold nanoparticle-based immunochromatographic assay (multi-AuNPs-ICA) was established for simultaneous detection of OXY and NIT in wastewater, milk, and beef. Visual and calculated limits of detection ranged from 2 to 20 ng/mL and 0.37-7.16 ng/mL, respectively. Spiked recovery rates in wastewater, milk, and beef samples were 102.6%-110.5%, 90.4%-115.6%, and 86.9%-117.7%, respectively. These were highly consistent with instrumental methods, confirming the reliability of multi-AuNPs-ICA and its significant potential for on-site rapid screening.
如何引用
AMA
Qingqing Liu, Liqiang Liu, Yangmei Liu, Maozhong Sun, Hua Kuang, Chuanlai Xu, et al. A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and Environmental Samples.. Journal of agricultural and food chemistry. 2026; doi:10.1021/acs.jafc.6c04857.
APA
Qingqing Liu, Liqiang Liu, Yangmei Liu, Maozhong Sun, Hua Kuang, Chuanlai Xu, et al (2026). A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and Environmental Samples.. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.6c04857
GB/T 7714
Qingqing Liu, Liqiang Liu, Yangmei Liu, Maozhong Sun, Hua Kuang, Chuanlai Xu, et al. A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and Environmental Samples.[J]. Journal of agricultural and food chemistry, 2026 doi:10.1021/acs.jafc.6c04857.
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