{“_”：“气味结合蛋白18有助于对Flonicamid及其代谢产物的耐受性相关反应。”，“i”：[“意大利蜜蜂”，“藁本”]}
{"_":"Odorant-Binding Protein 18 Contributes to Tolerance-Related Responses to Flonicamid and Its Metabolite in .","i":["Apis mellifera","ligustica"]}
摘要(中文译文)
蜜蜂依赖嗅觉感知信息素和环境化学物质。气味结合蛋白（OBPs）是气味识别过程中的关键载体，而越来越多的研究表明，OBPs的功能可能超越化学感受范畴，尤其在应对化学胁迫方面发挥作用。本研究中，亚致死剂量暴露于氟啶虫酰胺（FLO）或其代谢物4-（三氟甲基）烟酰胺（TFNA-AM）后，蜜蜂触角中AmOBP18的表达显著上调。荧光竞争结合实验显示，AmOBP18对TFNA-AM（解离常数= 0.65 ± 0.01 μM）和FLO（解离常数= 1.14 ± 0.03 μM）具有高结合亲和力。表面等离子体共振（SPR）分析证实了上述结果，TFNA-AM的解离常数为0.163 μM。此外，在大肠杆菌中过表达AmOBP18显著增强了对TFNA-AM的细菌耐受性。沉默AmOBP18则增加了蜜蜂对FLO和TFNA-AM的敏感性。我们证明，AmOBP18可能具有超越其经典嗅觉功能的作用，参与对氟啶虫酰胺和TFNA-AM的耐受相关反应。
Abstract
Honey bees rely on olfaction to perceive pheromones and environmental chemicals. Odorant-binding proteins (OBPs) are key carriers in odorant recognition, while growing studies suggest that OBPs may function beyond chemosensation, particularly in response to chemical stress. In this study, expression in the antennae of was significantly upregulated after sublethal exposure to flonicamid (FLO) or its metabolite 4-(trifluoromethyl) nicotinamide (TFNA-AM). Fluorescence competitive binding assays showed that AmOBP18 exhibited high binding affinity for TFNA-AM ( = 0.65 ± 0.01 μM) and FLO ( = 1.14 ± 0.03 μM). Surface plasmon resonance (SPR) analysis confirmed these findings, with a value of 0.163 μM for TFNA-AM. Moreover, overexpression of in significantly enhanced bacterial tolerance to TFNA-AM. Silencing of increased honey bee sensitivity to both FLO and TFNA-AM. We demonstrate that AmOBP18 may have functions beyond its classical olfactory role, contributing to tolerance-related responses to flonicamid and TFNA-AM.
如何引用
AMA
Peitong Chen, Ru Yan, Liangdong Wang, Jiaqi Huang, Yao Liu, Zhanyi Xu, et al. {"_":"Odorant-Binding Protein 18 Contributes to Tolerance-Related Responses to Flonicamid and Its Metabolite in .","i":["Apis mellifera","ligustica"]}. Journal of agricultural and food chemistry. 2026; doi:10.1021/acs.jafc.6c01650.
APA
Peitong Chen, Ru Yan, Liangdong Wang, Jiaqi Huang, Yao Liu, Zhanyi Xu, et al (2026). {"_":"Odorant-Binding Protein 18 Contributes to Tolerance-Related Responses to Flonicamid and Its Metabolite in .","i":["Apis mellifera","ligustica"]}. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.6c01650
GB/T 7714
Peitong Chen, Ru Yan, Liangdong Wang, Jiaqi Huang, Yao Liu, Zhanyi Xu, et al. {"_":"Odorant-Binding Protein 18 Contributes to Tolerance-Related Responses to Flonicamid and Its Metabolite in .","i":["Apis mellifera","ligustica"]}[J]. Journal of agricultural and food chemistry, 2026 doi:10.1021/acs.jafc.6c01650.
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