JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY农业与食品化学杂志
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY(英文缩写 J AGR FOOD CHEM),ISSN 0021-8561,eISSN 1520-5118,中文译名:农业与食品化学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-28 至 2026-09-28，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.895 | Q1 |
| 2022 | 6.100 | Q1 |
| 2023 | 5.700 | Q1 |
| 2024 | 6.200 | Q1 |
| 2025 | 6.700 | Q1 |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 最新收录文献
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1. Multifaceted Cross-Rational Modification of the α2,3-Sialyltransferase MsST for High-Efficiency Production of 3'-Sialyl-3-fucosyllactose via One-Pot Cascade Reaction.
PMID:日期:2026-09-243'-Sialyl-3-fucosyllactose (3'S3FL) is a representative fucose-containing sialylated human milk oligosaccharide with distinctive biological functions, but its in vitro synthesis is limited by the poor tolerance of α2,3-sialyltransferases toward fucosylated acceptors. Here, we report an engineered α2,3-sialyltransferase variant, R60V, with enhanced catalytic performance toward the 3-fucosyllactose (3-FL) substrate. R60V exhibited a 3.6-fold enhancement in transglycosylation activity and a nearly 5-fold lower apparent Km for 3-FL. Its half-life at 40 °C extended from 42.55 to 89.82 h. Mechanistic analyses revealed that R60V alleviated steric hindrance for 3-FL binding, promoted productive interactions with the catalytic residue, and balanced local flexibility with global stability. Furthermore, a one-pot cascade system comprising R60V and a CMP-sialic acid synthetase was established, enabling in situ sialyl donor generation and affording 9.31 mM 3'S3FL in 93.13% yield within 3 h under optimized conditions. This study provides a practical biocatalytic cascade platform for efficient 3'S3FL production.
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2. Genome-Edited Nuruk-Derived Yeast for Enhancing Quality and Flavor of Fermented Foods.
PMID:日期:2026-09-23While esters are known to provide fruity and floral characteristics, their production is often insufficient to meet consumer preferences. In this study, Saccharomyces cerevisiae N9 isolated from Korean nuruk (fermentation starter) was engineered using Cas9-based genome editing and applied to the production of Korean rice wine (makgeolli). URE2, coding for a transcriptional regulator of nitrogen catabolite repression, was deleted to increase the secretion of savory amino acids. ATF1, coding for alcohol acetyltransferase 1, was overexpressed to intensify the production of ester-based flavor compounds, whereas IAH1, coding for esterase, was deleted to increase ester accumulation. Ester intensity was further modulated in YPD medium by controlling ATF1 expression and mixed-culture ratios, while Makgeolli made with the URE2-deleted or ATF1/IAH1-engineered strains showed increased amino acid or ester levels. As no foreign DNA was introduced, these strains may not be subject to GM regulation in the United States, supporting their broader application in fermented foods.
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3. Design, Synthesis, and Biological Evaluation of Novel Biaryl-pyridine Derivatives with Potent Postemergence Herbicidal Activity.
PMID:日期:2026-09-22Protoporphyrinogen oxidase (PPO) is an established herbicide target. Oxygen-substituted biaryl-pyridine derivatives were designed and synthesized through scaffold optimization. Greenhouse assays identified several compounds with strong postemergence activity at 150 g/ha. Compound 6a retained 75% inhibition against three broadleaf weeds at 9.375 g/ha and caused generally less crop injury than acifluorfen. No mortality or behavioral abnormalities were observed in honey bees treated with 6a or (±)-6b at 100 μg/bee. Molecular docking, molecular dynamics simulations, and molecular mechanics/Poisson-Boltzmann surface area (MM-PBSA) analyses supported stable interactions of 6a and an (R)-6b model with Nicotiana tabacum PPO (NtPPO). Surface plasmon resonance (SPR) confirmed direct binding of 6a and (±)-6b to NtPPO, with KD values of 41.20 and 38.60 nM, respectively. Density functional theory (DFT) calculations further supported 6a as a promising herbicidal lead.
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4. Biosynthesis of Active Ginsenoside Aglycone Analogues Using Two Oxidases Mined from Mucor spinosus.
PMID:日期:2026-09-15The pharmacological activity of protopanaxadiol (PPD) can be enhanced by oxidative modification. Compared with chemical synthesis and biotransformation, synthetic biology offers a more efficient and eco-friendly approach for modifying PPD. In this study, we identified two oxidases from Mucor spinosus. MsSDR3 oxidizes C3-OH of both dammarenediol-II and PPD to a ketone, while MsCYP3 oxidizes C12-OH of PPD to a ketone and hydroxylates PPD at C7β, C15α, and C11β positions. Combining MsSDR3/MsCYP3 with the enzymes involved in ginsenoside biosynthesis, we achieved de novo biosynthesis of seven ginsenoside aglycone analogues in Saccharomyces cerevisiae. Pharmacological evaluation indicated that 12-oxo-15α-hydroxy-protopanaxadiol (p3) showed higher anticolon cancer activity, and 7β-hydroxy-protopanaxadiol (p6) exhibited not only higher anticolon, antigastric, antiliver, antilung, and antipancreatic cancer activities but also higher cardioprotective activity than PPD. Our work establishes a green and sustainable platform for producing active ginsenoside aglycone analogues, paving the way for the development of drugs and functional foods.
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5. Odorant-Binding Protein 56 h Contributes to the Behavioral Response of Frankliniella occidentalis to Neryl (S)-2-Methylbutanoate.
PMID:日期:2026-09-11The western flower thrips, Frankliniella occidentalis, poses a serious threat to global agriculture because of its feeding damage and virus transmission. Its major aggregation pheromone component neryl (S)-2-methylbutanoate (NMB) is a strong attractant for adult females. However, the molecular mechanisms underlying NMB detection, particularly the role of odorant-binding proteins, remain poorly defined. This study identified and functionally characterized FoccOBP56h as a key mediator of the behavioral response to NMB. qRT-PCR showed that FoccOBP56h is predominantly expressed in the antennae and is significantly upregulated following NMB exposure. Fluorescence binding assays revealed that recombinant FoccOBP56h exhibited high affinity for NMB. Molecular docking and site-directed mutagenesis indicated that Asn90, Met104, and Val105 are important for NMB binding. RNAi-mediated knockdown of FoccOBP56h significantly reduced female attraction to NMB. These findings identify FoccOBP56h as a promising molecular target involved in NMB detection, providing a foundation for developing semiochemical-based strategies for insect behavior manipulation in pest management.
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6. Polyethylenimine-Coated MXene Quantum Dots Integrate Anti-Oomycete Activity with Host Immunity Activation for Plant Oomycete Control.
PMID:日期:2026-09-09Pathogenic oomycetes can infect hundreds of crops, leading to significant economic losses globally. This study synthesized polyethylenimine-coated MXene quantum dots (PEI-MQDs) and investigated their antioomycete potential using the vegetable oomycete pathogen Phytophthora capsici as a model system. PEI-MQDs demonstrated potent antioomycete activity against P. capsici by damaging cellular structures and subverting asexual reproduction (zoosporangium/zoospore formation). Transcriptomic profiling demonstrated that treatment with PEI-MQDs induced extensive transcriptional changes in P. capsici genes, primarily affecting antioxidant activities, structural constituents, and metabolic pathways. Greenhouse pepper experiments showed that PEI-MQDs exerted both protective and curative effects against P. capsici infection, as effectively as the commercial oomycide metalaxyl. Additionally, PEI-MQDs can promote hot pepper immunity and growth through different pathways, as revealed by transcriptomic analysis. These findings make PEI-MQDs a novel nano-oomycide in agricultural applications, offering a sustainable platform for crop protection and expanding the functional scope of quantum dots in plant health.
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7. Identification of a Novel Thermal Promoter and Its Application in Glutamate Decarboxylase Protein Expression in Bacillus licheniformis.
PMID:日期:2026-09-09As a thermotolerant bacterium, Bacillus licheniformis is an attractive chassis for high-temperature biomanufacturing. Here, we identified a novel temperature-responsive promoter, PycgM, which maintained strong transcriptional activity at 37-52 °C. In a promoter-mCherry reporter system, PycgM exhibited 2287.3-fold higher activity than P2 at 52 °C, demonstrating excellent compatibility with a thermotolerant host. Truncation analysis identified a 150-bp core functional region responsible for optimal activity under induction and heat stress. When applied to drive glutamate decarboxylase expression at 50 °C, PycgM enabled γ-aminobutyric acid production of 391.67 g/L with a 98.69% conversion rate, representing a 275% increase over 37 °C fermentation. The whole-cell biocatalyst retained 86% activity after five reuse cycles, and SEM analysis indicated acceptable structural stability despite moderate morphological changes. These results demonstrate that PycgM is a robust, high-temperature genetic element for efficient enzyme and metabolite production in thermotolerant hosts.
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8. Domain-Swapping of a GT-B Glycosyltransferase Broadens Its Sugar Donor Range for Glycodiversification of Natural Products.
8. GT-B糖基转移酶的结构域交换拓宽了其糖基供体范围以用于天然产物的糖基多样化PMID:日期:2026-09-09Glycosylation of natural compounds modulates their bioactivity as well as their physicochemical properties, making glycodiversification a valuable strategy for identifying glycosidic derivatives with improved functional profiles. However, the use of glycosyltransferases (GTs) for their synthesis is constrained by stringent sugar nucleotide donor selectivity, which limits the structural diversity of accessible glycosides. The chimeric NB1//CC1, constituted of 2 self-assembled domains originating from plant glycosyltransferases, was previously demonstrated to transfer glucose onto a wider range of natural compounds than its parental enzymes. This study demonstrates that this chimeric enzyme also exhibits a relaxed selectivity toward sugar nucleotide donors. This donor promiscuity can further be enhanced by the Mg2+ cation, which increases the sugar transfer rate for the chimeric enzyme. NB1//CC1 can therefore be used as a single biocatalyst to generate a library of glycosidic variants of a given natural glycosylated compound.
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9. The Checkpoint Kinase SsChk2 Mediates Tebuconazole Resistance via Modulating Ergosterol Biosynthesis in Sclerotinia sclerotiorum.
PMID:日期:2026-09-09Sclerotinia stem rot caused by Sclerotinia sclerotiorum severely constrains global crop production. This study explored the regulatory role of checkpoint kinase SsChk2 in tebuconazole resistance. Bioassays indicated that ΔSsChk2 mutants showed reduced sensitivity to three demethylation inhibitors (DMIs), compared with wild-type strain UF-1, while maintaining normal biological phenotypes. Transcription of SsChk2 was significantly suppressed in five UV-induced tebuconazole-resistant mutants and inhibitor-treated UF-1. Independent of SsCYP51 amino acid substitutions, the ΔSsChk2 strain exhibited elevated SsCYP51 expression, ergosterol accumulation, and cellular structural stability under tebuconazole stress. RNA-seq analyses revealed that SsChk2 deletion upregulated key ergosterol biosynthesis genes and ABC1/MFS2 transporters and activated coordinated defensive pathways. These findings reveal that loss or downregulation of SsChk2 stimulates ergosterol biosynthesis to confer DMI resistance and uncover a promising target for sustainable control of Sclerotinia stem rot.
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10. Amination of Epigallocatechin Gallate and Dihydromyricetin in a Caenorhabditis elegans Culture Medium Causes Experimental Artifacts by Reducing the Free Amino Acid Content.
PMID:日期:2026-09-09Pyrogallol-type polyphenols undergo Strecker degradation with amino acids in cell culture media, yet whether such chemistry measurably alters the nutritional composition of Caenorhabditis elegans growth medium and affects worm phenotypes remains unclear. Using a tert-butyldimethylsilyl derivatization-based GC-MS workflow, we analyzed five flavonoids with distinct B-ring hydroxylation patterns in the nematode growth medium (NGM): epigallocatechin gallate (EGCG), dihydromyricetin (DHM), quercetin, luteolin, and apigenin. Within 2 h, 400 μM EGCG and DHM reduced multiple amino acids in NGM, with concurrent formation of the aminated derivatives N-EGCG and N-DHM identified by LC-MS/MS. This effect persisted over 72 h in OP50-seeded NGM and propagated to worms, lowering endogenous amino acids together with lactate and pyruvate, and ultimately delaying growth, development, and reproductive capacity. These findings highlight that polyphenol bioactivity studies should not overlook chemical reactions between polyphenols and medium constituents to avoid erroneous conclusions.