早期膝骨性关节炎滑膜液IL-16和RANTES/CCL5信号：一项试点抗体阵列研究
Synovial fluid IL-16 and RANTES/CCL5 signals in early knee osteoarthritis: a pilot antibody-array study.
摘要(中文译文)
早期骨关节炎（OA）可靠的分子指标仍然有限。由于滑液（SF）反映关节内免疫微环境，炎症信号可能有助于描述与早期OA相关的初步特征，以供未来验证。这项试点性、假设生成研究使用高通量抗体芯片对膝关节OA不同影像学分组的滑液细胞因子进行了分析。共纳入16名参与者：K-L 0级非影像学对照组（K0，n=4）、早期OA组（K1；Kellgren-Lawrence 1-2级，n=6）和晚期OA组（K3；3-4级，n=6）。使用RayBiotech人炎症抗体芯片检测了40种炎症蛋白。在K1与K0比较中，经Benjamini-Hochberg校正后仍具有显著性的蛋白仅为IL-16[倍数变化（FC）=15.79；logFC=3.98；logFC的近似不确定区间为2.21至5.75；校正P值=0.027]和RANTES/CCL5（FC=11.83；logFC=3.56；logFC的近似不确定区间为-1.23至8.36；校正P值=0.027）。基于所选蛋白的PCA和热图分析仅用于对所选信号进行可视化，而非作为诊断区分能力的证据。在K3与K1比较中，有7种蛋白基于原始P值显示探索性下调，但未通过多重检验校正。因此，GO/KEGG分析仅被解释为补充性假设生成。在这个样本量非常小的试点队列中，IL-16和RANTES/CCL5在多重检验校正后显示出与早期OA相关的芯片检测炎症信号。这些发现并不构成对临床标志物的验证，也未作出任何诊断性声明，因为未进行ELISA确认、ROC分析，也没有独立验证队列。因此，这些数据应被视为初步发现结果，在考虑临床转化之前，需要进行更大规模、把握度充分且采用正交定量验证的研究。
Abstract
Reliable molecular indicators for early osteoarthritis (OA) remain limited. Because synovial fluid (SF) reflects the intra-articular immune microenvironment, inflammatory signals may help describe preliminary features associated with early OA for future validation. This pilot, hypothesis-generating study used a high-throughput antibody array to profile SF cytokines across radiographic groups of knee OA. Sixteen participants were included: a K-L grade 0 non-radiographic comparator group (K0, n = 4), early OA (K1; Kellgren-Lawrence grade 1-2, n = 6), and late OA (K3; grade 3-4, n = 6). Forty inflammatory proteins were measured using the RayBiotech Human Inflammation Antibody Array. In K1 versus K0, IL-16 [fold change (FC) = 15.79; logFC = 3.98; approximate uncertainty interval for logFC, 2.21 to 5.75; adj.P.Val = 0.027] and RANTES/CCL5 (FC = 11.83; logFC = 3.56; approximate uncertainty interval for logFC, -1.23 to 8.36; adj. P.Val = 0.027) were the only proteins that remained significant after Benjamini-Hochberg correction. PCA and heatmap analyses based on selected proteins were used only as visualization of selected signals and not as evidence of diagnostic discrimination. In K3 versus K1, seven proteins showed exploratory downregulation based on raw P values but did not survive multiple-testing correction. GO/KEGG analyses were therefore interpreted as supplementary hypothesis generation only. In this very small pilot cohort, IL-16 and RANTES/CCL5 showed array-based inflammatory signals associated with early OA after multiple-testing correction. The findings do not constitute validation of a clinical marker, and no diagnostic claim is made because ELISA confirmation, ROC analysis, and an independent validation cohort were not available. These data should therefore be regarded as preliminary discovery results, and larger, adequately powered studies with orthogonal quantitative validation are required before clinical translation can be considered.
如何引用
AMA
Bei Lin, Boyue Wu, Huihuang Chen, Wentao Lin, Dasheng Lin, Eryou Feng. Synovial fluid IL-16 and RANTES/CCL5 signals in early knee osteoarthritis: a pilot antibody-array study.. Frontiers in immunology. 2026; doi:10.3389/fimmu.2026.1889340.
APA
Bei Lin, Boyue Wu, Huihuang Chen, Wentao Lin, Dasheng Lin, Eryou Feng (2026). Synovial fluid IL-16 and RANTES/CCL5 signals in early knee osteoarthritis: a pilot antibody-array study.. Frontiers in immunology. https://doi.org/10.3389/fimmu.2026.1889340
GB/T 7714
Bei Lin, Boyue Wu, Huihuang Chen, Wentao Lin, Dasheng Lin, Eryou Feng. Synovial fluid IL-16 and RANTES/CCL5 signals in early knee osteoarthritis: a pilot antibody-array study.[J]. Frontiers in immunology, 2026 doi:10.3389/fimmu.2026.1889340.
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