中国西北地区苯并咪唑抗性扭体血蜱E198A突变的优势和共选择的出现
{"_":"Dominance of the E198A Mutation and Emergence of Co-Selection in Benzimidazole-Resistant from Northwestern China.","i":["Haemonchus contortus"]}
摘要(中文译文)
新疆伊犁地区胃肠道线虫对苯并咪唑（BZ）的耐药性是全球绵羊生产的主要制约因素。然而，关于中国西北部重要畜牧区新疆伊犁州耐药性流行率及分子机制的数据仍然有限。本研究旨在确定昭苏县和特克斯县虫群中BZ耐药相关单核苷酸多态性（SNPs）的发生频率。从当地屠宰场自然感染绵羊中采集成年雄虫（n = 150）。通过测序内转录间隔区2（ITS-2）进行形态学物种鉴定。扩增并测序异型-1 β-微管蛋白基因的385 bp片段，以检测第167位（F167Y）、198位（E198A）和200位（F200Y）密码子的SNPs。所有个体中均未发现F167Y突变。相比之下，E198A突变发生频率极高，昭苏县耐药等位基因频率（RAF）为64.7%，特克斯县为52.7%。F200Y突变表现出明显的地理差异：昭苏县较低（RAF = 9.3%），而特克斯县显著较高（RAF = 33.3%）。单倍型分析揭示，昭苏县耐药性主要由E198A突变驱动，而特克斯县虫群则表现出E198A和F200Y共同选择的复杂模式，双重杂合个体比例较高（29.3%）。本研究提供了伊犁州昭苏县和特克斯县虫群中严重BZ耐药性的全面分子证据。E198A突变的显著优势以及特克斯县多位点耐药性的出现，表明耐药性正以超出历史水平的速率快速升级。这些发现提示苯并咪唑类药物在该地区可能无效，并凸显了修订当地寄生虫控制策略的紧迫性。
Abstract
Benzimidazole (BZ) resistance in the gastrointestinal nematode is a major constraint to sheep production worldwide. However, data on the prevalence and molecular mechanisms of resistance in Yili Prefecture, Xinjiang-a key livestock region in Northwestern China-remain limited. This study aimed to determine the frequency of BZ resistance-associated single nucleotide polymorphisms (SNPs) in populations from Zhaosu and Tekesi counties. Adult male worms (n = 150) were collected from naturally infected sheep at local abattoirs. Species identity was confirmed morphologically by sequencing the internal transcribed spacer 2 (ITS-2) region. A 385 bp fragment of the isotype-1 β-tubulin gene was amplified and sequenced to detect SNPs at codons 167 (F167Y), 198 (E198A), and 200 (F200Y). The F167Y mutation was absent in all individuals. In contrast, the E198A mutation occurred at exceptionally high frequencies, with resistant allele frequencies (RAF) of 64.7% in Zhaosu and 52.7% in Tekesi. The F200Y mutation showed clear geographical variation: it remained low in Zhaosu (RAF = 9.3%) but was substantially higher in Tekesi (RAF = 33.3%). Haplotype analysis revealed that resistance in Zhaosu was driven primarily by the E198A mutation, whereas the Tekesi population exhibited complex patterns of co-selection of both E198A and F200Y, with a high proportion of double-heterozygous individuals (29.3%). This study provides comprehensive molecular evidence of severe BZ resistance in populations from Zhaosu and Tekesi counties, Yili Prefecture. The marked predominance of the E198A mutation, together with the emergence of multi-locus resistance in Tekesi, indicates a rapid escalation of resistance beyond historical levels. These findings suggest that benzimidazoles are likely ineffective in this region and highlight the urgent need to revise local parasite control strategies.
如何引用
AMA
Waresi Tuersong, Lianxi Xin, Abudusaimaiti Tuoheti, Ailixire Maimaiti, Dilare Xuekelaiti, Reyilanmu Tuerhong, et al. {"_":"Dominance of the E198A Mutation and Emergence of Co-Selection in Benzimidazole-Resistant from Northwestern China.","i":["Haemonchus contortus"]}. Veterinary sciences. 2026; doi:10.3390/vetsci13060603.
APA
Waresi Tuersong, Lianxi Xin, Abudusaimaiti Tuoheti, Ailixire Maimaiti, Dilare Xuekelaiti, Reyilanmu Tuerhong, et al (2026). {"_":"Dominance of the E198A Mutation and Emergence of Co-Selection in Benzimidazole-Resistant from Northwestern China.","i":["Haemonchus contortus"]}. Veterinary sciences. https://doi.org/10.3390/vetsci13060603
GB/T 7714
Waresi Tuersong, Lianxi Xin, Abudusaimaiti Tuoheti, Ailixire Maimaiti, Dilare Xuekelaiti, Reyilanmu Tuerhong, et al. {"_":"Dominance of the E198A Mutation and Emergence of Co-Selection in Benzimidazole-Resistant from Northwestern China.","i":["Haemonchus contortus"]}[J]. Veterinary sciences, 2026 doi:10.3390/vetsci13060603.
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