超临界CO2脱细胞羊膜ECM水凝胶促进免疫调节皮肤再生
{"_":"Supercritical CO-Decellularized amniotic ECM hydrogel promotes immunomodulatory skin regeneration.","sub":["2"]}
摘要(中文译文)
急性紫外线B（UVB）暴露可触发快速皮肤炎症，其特征为免疫细胞浸润、细胞外基质（ECM）降解及组织功能障碍。尽管当前的治疗策略如保湿敷料、透明质酸/胶原蛋白制剂及外源性生长因子可提供部分益处，但由于其调节受损皮肤炎症与再生微环境的能力有限，不足以促进全面再生。本研究提出一种通过超临界二氧化碳（scCO）脱细胞技术工程化的脱细胞猪羊膜（dPAM）水凝胶。该方法有效去除免疫原性细胞成分，同时保留ECM相关生物活性分子，包括VEGF、PDGF、IL-10及核心蛋白聚糖（Decorin）。值得注意的是，dPAM水凝胶保留了基质结合蛋白如Decorin，该蛋白与组织再生及参与毛囊生物学的Wnt相关信号传导有关。研究证明，dPAM水凝胶促进内皮细胞血管生成并减弱巨噬细胞中M1相关促炎反应。此外，它增强了人毛囊来源真皮乳头细胞（HFDPCs）中促再生及毛囊相关基因反应，提示dPAM水凝胶可能影响Wnt相关再生信号传导。在UVB诱导的皮肤炎症小鼠模型中，该水凝胶表现出双重功能，即减弱早期免疫反应并促进组织修复。综上所述，这些发现凸显dPAM水凝胶作为一种基于ECM的生物活性平台，能够为皮肤修复创造促再生微环境，在光损伤相关皮肤损伤及更广泛的再生生物材料策略中具有潜在应用前景。
Abstract
Acute ultraviolet B (UVB) exposure triggers rapid skin inflammation, characterized by immune cell infiltration, extracellular matrix (ECM) degradation, and tissue dysfunction. Although current therapeutic strategies such as hydrating dressings, hyaluronic acid/collagen formulations, and exogenous growth factors provide partial benefits, they are insufficient to promote comprehensive regeneration due to their limited ability to modulate the inflammatory and regenerative microenvironment of damaged skin. In this study, we present a decellularized porcine amniotic membrane (dPAM) hydrogel engineered via supercritical carbon dioxide (scCO)-based decellularization. This method effectively removes immunogenic cellular components while preserving ECM-associated bioactive molecules, including VEGF, PDGF, IL-10, and Decorin. Notably, the dPAM hydrogel preserved matrix-bound proteins such as Decorin, which have been associated with tissue regeneration and Wnt-related signaling involved in hair follicle biology. studies demonstrated that the dPAM hydrogel promotes angiogenesis in endothelial cells and attenuates M1-associated pro-inflammatory responses in macrophages. Moreover, it enhanced pro-regenerative and hair follicle-associated gene responses in human follicle-derived dermal papilla cells (HFDPCs), suggesting that dPAM hydrogel may influence Wnt-related regenerative signaling. In a UVB-induced skin inflammation murine model, the hydrogel exhibited dual functionality by attenuating early immune responses and promoting tissue repair. Collectively, these findings highlight the dPAM hydrogel as a bioactive ECM-based platform capable of creating a pro-regenerative microenvironment for cutaneous repair, with potential applications in photodamage-associated skin injury and broader regenerative biomaterial strategies.
如何引用
AMA
Seongryeol Ye, Yu-Jin Kim, Jin Yoo, In Cheul Choi, Kangwon Lee, Jong Woong Park, et al. {"_":"Supercritical CO-Decellularized amniotic ECM hydrogel promotes immunomodulatory skin regeneration.","sub":["2"]}. Materials today. Bio. 2026; doi:10.1016/j.mtbio.2026.103293.
APA
Seongryeol Ye, Yu-Jin Kim, Jin Yoo, In Cheul Choi, Kangwon Lee, Jong Woong Park, et al (2026). {"_":"Supercritical CO-Decellularized amniotic ECM hydrogel promotes immunomodulatory skin regeneration.","sub":["2"]}. Materials today. Bio. https://doi.org/10.1016/j.mtbio.2026.103293
GB/T 7714
Seongryeol Ye, Yu-Jin Kim, Jin Yoo, In Cheul Choi, Kangwon Lee, Jong Woong Park, et al. {"_":"Supercritical CO-Decellularized amniotic ECM hydrogel promotes immunomodulatory skin regeneration.","sub":["2"]}[J]. Materials today. Bio, 2026 doi:10.1016/j.mtbio.2026.103293.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出