贻贝启发的同轴电纺纳米纤维膜通过增强巨噬细胞M2极化来促进无瘢痕口腔黏膜修复
Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization.
摘要(中文译文)
口腔黏膜缺损的修复常因传统材料在湿润、动态环境中无法保持黏附而受到阻碍。受贻贝湿黏附特性的启发，我们通过同轴静电纺丝开发了一种仿生自黏性纳米纤维膜。该膜以聚己内酯（PCL）为芯层提供机械强度，以多巴胺（DA）修饰的甲基丙烯酰化明胶（GelMA）为壳层提供生物相容性和黏附性。实验结果表明，该膜具有优异的力学性能，拉伸强度为2.52 MPa，溶胀度为554.6%。2% DA的引入使湿黏附强度显著提高至30.53 kPa，能够稳定黏附于口腔组织超过24 h。实验证明其具有良好的细胞相容性，并可促进成纤维细胞黏附和增殖。采用小鼠皮肤和大鼠口腔黏膜缺损模型的研究显示，其可加速伤口闭合、减少瘢痕形成并改善组织再生。免疫学分析表明，DA修饰的纳米纤维膜通过促进巨噬细胞向抗炎M2表型极化来调节局部免疫微环境。转录组分析及后续分子验证确定，过氧化物酶体增殖物激活受体γ（PPARγ）信号通路的激活是这一免疫调节效应的关键机制。这些结果表明，受贻贝启发的同轴纳米纤维膜可提供稳定的湿黏附并主动调节免疫反应，以促进口腔黏膜缺损的无瘢痕愈合，凸显了其作为软组织修复功能生物材料的潜力。
Abstract
The repair of oral mucosal defects is often hindered by the failure of traditional materials to maintain adhesion in humid, dynamic environments. Inspired by the wet-adhesion properties of mussels, we developed a biomimetic self-adhesive nanofibrous membrane via coaxial electrospinning. This membrane utilizes a polycaprolactone (PCL) core for mechanical strength and a dopamine (DA)-modified gelatin methacrylate (GelMA) shell for biocompatibility and adhesion. Experimental results demonstrate that the membrane possesses excellent mechanical properties, with a tensile strength of 2.52 MPa and a swelling degree of 554.6%. The incorporation of 2% DA significantly enhanced wet-adhesive strength to 30.53 kPa, allowing for stable adhesion to oral tissues for over 24 h. experiments demonstrated good cytocompatibility and promoted fibroblast adhesion and proliferation. studies using murine skin and rat oral mucosal defect models showed accelerated wound closure, reduced scar formation, and improved tissue regeneration. Immunological analyses revealed that the DA-modified nanofiber membrane modulated the local immune microenvironment by promoting macrophage polarization toward the anti-inflammatory M2 phenotype. Transcriptomic analysis and subsequent molecular validation identified activation of the peroxisome proliferator-activated receptor gamma (PPARγ) signaling pathway as a key mechanism underlying this immunoregulatory effect. These results demonstrate that the mussel-inspired coaxial nanofiber membrane provides stable wet adhesion and actively regulates immune responses to promote scarless healing of oral mucosal defects, highlighting its potential as a functional biomaterial for soft tissue repair.
如何引用
AMA
Junpeng Chen, Jinpeng Jiang, Wenyi Shen, Bingjie Xu, Haina Miao, Yangxi Cheng, et al. Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization.. Materials today. Bio. 2026; doi:10.1016/j.mtbio.2026.103272.
APA
Junpeng Chen, Jinpeng Jiang, Wenyi Shen, Bingjie Xu, Haina Miao, Yangxi Cheng, et al (2026). Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization.. Materials today. Bio. https://doi.org/10.1016/j.mtbio.2026.103272
GB/T 7714
Junpeng Chen, Jinpeng Jiang, Wenyi Shen, Bingjie Xu, Haina Miao, Yangxi Cheng, et al. Mussel-inspired coaxial electrospun nanofiber membranes promote scarless oral mucosal repair by enhancing macrophage M2 polarization.[J]. Materials today. Bio, 2026 doi:10.1016/j.mtbio.2026.103272.
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