一种用于急性肾损伤中实时ROS成像和线粒体复苏驱动的屏障修复的膜融合治疗纳米平台
A membrane-fused theranostic nanoplatform for real-time ROS imaging and mitochondrial resuscitation-driven barrier repair in acute kidney injury.
摘要(中文译文)
急性肾损伤（AKI）的有效管理目前受限于缺乏能够同时实现诊断与结构修复的统一平台。为应对这一挑战，通过膜融合策略构建了一种仿生诊疗纳米平台——载硫辛酸类外泌体纳米颗粒（EXLNPs），该策略将间充质干细胞（MSC）来源外泌体的归巢能力与合成脂质体的高负载效率协同结合。给药后，EXLNPs作为自报告诊断剂，在高活性氧（ROS）微环境中表现出实时荧光激活，且与损伤严重程度准确相关。在治疗方面，膜融合EXLNPs通过协调精准的“检测与修复”级联反应，优于天然外泌体。它们有效清除ROS以逆转线粒体代谢崩溃，从而恢复氧化磷酸化能力及ATP生成，这对于上皮紧密连接蛋白（Occludin 1和15、CLDN1和15）的能量依赖性重新组装至关重要。线粒体-屏障轴的恢复显著改善了缺血性AKI模型中的肾小管坏死并恢复了肾功能。这些发现表明，这种生物合成杂合策略为急性肾脏疾病的精准诊断和代谢重编程提供了一种强效且具有临床相关性的解决方案。
Abstract
Effective management of Acute Kidney Injury (AKI) is currently limited by the lack of unified platforms capable of simultaneous diagnosis and structural repair. Addressing this challenge, a biomimetic theranostic nanoplatform, Lipoic Acid-loaded Exosome-Like Nanoparticles (EXLNPs), was engineered via a membrane fusion strategy wherein the homing capability of MSC-derived (Mesenchymal Stem Cells-derived) exosomes is synergized with the high-loading efficiency of synthetic liposomes. Upon administration, EXLNPs acted as a self-reporting diagnostic agent, exhibiting real-time fluorescence activation in response to high-ROS microenvironments, which correlated accurately with injury severity. Therapeutically, the membrane-fused EXLNPs outperformed native exosomes by orchestrating a precision "detect-and-repair" cascade. They effectively scavenged ROS to reverse mitochondrial metabolic collapse, thereby restoring the oxidative phosphorylation capacity and ATP production essential for the energy-dependent re-assembly of epithelial tight junctions (Occludin 1 and 15, CLDN1 and 15). This restoration of the mitochondrial-barrier axis significantly ameliorated tubular necrosis and recovered renal function in ischemic AKI models. These findings demonstrate that this bio-synthetic hybrid strategy offers a potent, clinically relevant solution for the precision diagnosis and metabolic reprogramming of acute renal diseases.
如何引用
AMA
Fan Wu, Yi Shen, Liang Dong, Wei Nie, Wei Xue. A membrane-fused theranostic nanoplatform for real-time ROS imaging and mitochondrial resuscitation-driven barrier repair in acute kidney injury.. Materials today. Bio. 2026; doi:10.1016/j.mtbio.2026.103217.
APA
Fan Wu, Yi Shen, Liang Dong, Wei Nie, Wei Xue (2026). A membrane-fused theranostic nanoplatform for real-time ROS imaging and mitochondrial resuscitation-driven barrier repair in acute kidney injury.. Materials today. Bio. https://doi.org/10.1016/j.mtbio.2026.103217
GB/T 7714
Fan Wu, Yi Shen, Liang Dong, Wei Nie, Wei Xue. A membrane-fused theranostic nanoplatform for real-time ROS imaging and mitochondrial resuscitation-driven barrier repair in acute kidney injury.[J]. Materials today. Bio, 2026 doi:10.1016/j.mtbio.2026.103217.
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