CURRENT OPINION IN CHEMICAL BIOLOGY化学生物学新见
CURRENT OPINION IN CHEMICAL BIOLOGY(英文缩写 CURR OPIN CHEM BIOL),ISSN 1367-5931,eISSN 1879-0402,中文译名:化学生物学新见 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 8.972 | Q1 |
| 2022 | 7.800 | Q1 |
| 2023 | 6.900 | Q1 |
| 2024 | 6.100 | Q1 |
| 2025 | 7.200 | Q1 |
CURRENT OPINION IN CHEMICAL BIOLOGY 最新收录文献
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1. Enzymatic metal hydride and hydrogen atom transfer reactions: recent developments for synthetic applications.
PMID:日期:2026-10-01Metal-hydrogen (M-H) intermediates are central to many organometallic processes but only play a transient role in metalloenzyme mechanisms in Nature and cannot be used directly for synthetic purposes. However, recent advances have revealed that robust zinc and iron metalloproteins can be repurposed to access M-H intermediates for conversion of unsaturated organic moieties. This perspective surveys the developing field of M-H-driven biocatalysis, focusing on metal hydride and metal hydrogen atom transfer (MHAT) reactions. The ease with which M-H intermediates can be generated at enzyme metal centres is particularly striking, requiring only addition of simple organohydrosilane or borohydride co-substrates. We first explore how heterolytic enzyme M-H cleavage enables stereoselective ketone, alkene, and imine reductions, emulating classical nicotinamide adenine dinucleotide phosphate (NAD(P)H)-dependent oxidoreductase chemistry. We then survey remarkable reports of homolytic M-H cleavage as a route to radical hydrofunctionalisation, cyclisation, and hydrogenation of unactivated alkenes. Whilst mechanistically impressive, our perspective also shows that this new reactivity is scalable and evolvable for improved substrate scope and stereoselectivity. Finally, we discuss how emerging cobalt-based systems are opening pathways to new-to-nature catalysis, including Birch-like reductive dearomatisation. Collectively, these developments establish enzymatic M-H chemistry as a powerful, practical, and rapidly evolving platform for implementing biocatalysis in synthesis.
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2. Emerging classes of copper enzymes.
PMID:日期:2026-10-01Copper-dependent enzymes catalyze some of nature's most challenging oxidative transformations, yet many copper cofactors lack distinctive spectroscopic signatures or are kinetically labile, complicating their discovery and mechanistic characterization. Recent advances in genome mining, heterologous expression, and protein structural prediction have accelerated the identification of previously unrecognized copper enzymes. This Current Opinion highlights two emerging families of binuclear type 2 copper enzymes: plant-specific BURP domain peptide cyclases (BpCs) and fungal DUF3328 dicopper oxidative enzymes (DDOEs). Both families couple dioxygen reduction to oxidative chemistry through conserved dicopper active sites and catalyze oxidative peptide crosslinking; DDOEs additionally perform hydroxylation, halogenation, oxidative biaryl crosslinking, and, putatively, desaturation. By comparing these plant and fungal enzymes, we establish a unified framework for their biological functions, active-site architectures, dioxygen reduction/activation strategies, catalytic mechanisms, and DDOE nomenclature. Together, these enzymes redefine the scope of copper-dependent oxidation and provide new opportunities for biocatalysis, bioengineering, and synthetic biology.
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3. Advances in the DMSO reductase family: From discovery to mechanism.
PMID:日期:2026-09-29DMSO reductase (DMSOR) family found in prokaryotes is the most diverse group of molybdenum cofactor (Moco) containing enzymes from the structural as well as the catalytic viewpoints. While DMSOR, the prototype member of its family, is well characterized thanks to decades of rigorous research, a large majority of this family are yet to be characterized. These enzymes are important in biogeochemical cycles, biotechnology and health. With the discovery of new DMSOR family members, the family continues to grow further underscoring their importance. This review will focus on the latest developments in the DMSOR reductase family.
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4. Detecting replication-associated single-stranded DNA.
4. 检测复制相关的单链DNAPMID:日期:2026-09-29Single-stranded DNA (ssDNA) is used as a readout of replication stress, yet it arises in several contexts. It is a normal intermediate of DNA replication but can accumulate and persist after replication challenges and is also generated by replication-independent processes. These structures differ in strand origin, architecture, biological consequence, and therapeutic response, so ssDNA detection alone rarely identifies the underlying molecular intermediate or how it was generated. Here, we describe the principal sources of replication-associated ssDNA and review how different methods capture distinct aspects of these intermediates, including ssDNA exposure and accessibility, DNA termini, and their broader structural and replication context. As these features are shared across intermediates, individual readouts often capture multiple structures rather than uniquely identifying one. Reliable interpretation therefore requires integrating complementary signals with experimental perturbations and replication context. Future methods combining these features on individual DNA molecules may enable direct molecular classification of replication intermediates.
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5. Peptide-mediated CRISPR delivery: From dish to bloodstream.
5. 肽介导的CRISPR递送：从培养皿到血液PMID:日期:2026-09-24CRISPR-based therapeutics have advanced rapidly, but clinical translation remains bottlenecked by delivery. Standard carriers like viral vectors and lipid nanoparticles (LNPs) face challenges regarding cargo capacity, immunogenicity, and restricted tissue tropism. Peptide-mediated delivery platforms offer a highly tunable, non-viral alternative. These chemically defined sequences are uniquely modular: they are small enough to penetrate dense tissues, inexpensive to synthesize, and open to redesign residue by residue. In cell culture, peptide platforms compete effectively against standard electroporation, delivering high editing efficiency in primary human cells while significantly improving cell viability. Despite these in vitro successes, translating peptide-mediated delivery in vivo introduces physiological barriers. When introduced by tissue injection in vivo, peptide delivered CRISPR editors must tackle more barriers than mere cell entry and endosomal escape they had to face in the dish. They typically reach modest editing efficiencies, which, however, could still yield a therapeutic benefit. For systemic delivery, more fragile but transient ribonucleoprotein (RNP) complexes face hepatic clearance, phagocytic capture, and protein corona interference-obstacles that are driving a shift toward engineered nanoassemblies and mRNA/DNA cargo alternatives. This review outlines the evolution of peptide-based CRISPR delivery from cell culture to local and systemic in vivo applications, evaluating physiological boundaries and highlighting engineering avenues required to overcome them. Looking forward, the next decade of peptide-mediated delivery must reconcile the conservative demands of clinical translation with radical chemical innovation. To advance, the field must transition past qualitative readouts and integrate isolated advancements into a single cohesive vehicle engineered for robust in vivo efficacy. By systematically combining these chemical innovations, engineered peptides might overcome the restrictions of viruses and LNPs, providing a truly versatile platform for systemic CRISPR delivery.
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6. Chemical modification as a tool in nonaqueous enzymology.
PMID:日期:2026-09-24Nonaqueous solvents have been widely regarded as incompatible media for enzymes. Nevertheless, there is now an extensive body of literature on nonaqueous activity of enzymes in living systems as well as tools of chemistry and biology enhancing the compatibility of enzymatic processes in nonaqueous environments. Likewise, a series of chemical modification development campaigns have demonstrated the power of nonaqueous approaches, offering a unique opportunity in a broad array of fields spanning chemical biology, enzyme engineering, food science, nanoscience, and sustainable chemistry. In this Opinion, we discuss the current state of such nonaqueous chemical modification strategies by highlighting recent reports.
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7. From DNA origami to nanostructured mRNA-based delivery platforms.
PMID:日期:2026-09-22DNA origami technique has established programmable nucleic acid nanostructures as a promising platform for biomedical applications. Incorporating RNA as the origami scaffold extends this potential into direct cytoplasmic activity, enabling transient protein expression without nuclear entry. However, RNA's distinct molecular structure and chemical lability impose design constraints with no equivalent in DNA-only systems, and the criteria for achieving robust intracellular gene expression from these hybrid constructs remain incompletely understood. This review synthesizes the current design principles governing mRNA-DNA hybrid origami assembly, including helicity adjustment, crossover geometry and folding condition optimization. It also examines the structural requirements for translational competence, where staple coverage of the 5' UTR eliminates expression and endosomal escape remains the primary delivery bottleneck. While hybrid origami systems have advanced, their immunological profile remains uncharacterized, and manufacturing scalability is unaddressed. Recent evidence that mRNA compaction alone can substantially enhance protein production in vivo suggests that scaffold-directed folding can offer advantages beyond nuclease protection. Establishing design rules that co-optimize structural integrity, translation competence, and immunological compatibility will be essential for translating mRNA scaffolded structures toward therapeutic applications.
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8. Recent advances in the precision synthesis of homogeneous N-linked glycoproteins: Synthetic strategies and discovery of latent functions.
PMID:日期:2026-09-22Protein glycosylation crucially regulates the function and stability of mammalian membrane and secreted proteins. However, because biological glycan processing is inherently a non-template-driven process, natural glycoproteins exist as heterogeneous mixtures. This structural heterogeneity has historically hindered the precise study of specific glycoforms. To overcome this fundamental limitation, advanced chemical and semi-synthetic strategies have emerged, now enabling the precise construction of structurally homogeneous glycoproteins. In this review, we highlight recent progress in the chemical and semi-synthetic assembly of these pure glycoforms, with a focus on advanced peptide ligation technologies. Ultimately, by securing scalable access to well-defined synthetic models, these precision techniques are uncovering latent physiological functions and laying the groundwork for novel, highly targeted therapeutics.
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9. Druggable secondary sites in C-type lectin-like domains: Mechanistic insights and opportunities for chemical modulation.
PMID:日期:2026-09-18C-type lectins are pivotal orchestrators of immunity and homeostasis. However, developing potent small molecule modulators remains a significant challenge, as their primary binding sites are shallow and solvent-exposed. Recent biophysical and computational advancements have revealed that the intrinsic structural plasticity of the C-type lectin-like domains can be leveraged to target these receptors through alternative sites with superior druggability. This review outlines recent progress on the discovery of secondary sites in C-type lectins and highlights mechanistic insights into first-in-class inhibitors and activators for the receptors DC-SIGN, langerin, NKG2D and LOX-1. Moreover, we discuss the conformational plasticity of the mannose receptor and MGL and the unexplored opportunities of targeting these receptors via secondary sites. By engaging these secondary sites, certain limitations of traditional glycomimetics can be circumvented, thereby expanding the druggable landscape of the CTL superfamily.
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10. Multiplexed molecular imaging for spatial transcriptomics and proteomics.
PMID:日期:2026-09-18Spatial biology addresses a fundamental question of how individual biomolecules work together to create complex organisms. One of its major tasks is to localize diverse molecular targets in their native environment. Here we review latest technologies that enable high-throughput, multiplexed, molecular imaging of transcripts and proteins in biological samples. This review focuses on the evolving forefront of multiplexed imaging developed for biomedical research, encompassing a wide range of labeling strategies. We believe that this field has abundant opportunities for multidisciplinary innovations toward spatial omics at single-molecule resolution.