蓝细菌中不依赖光系统I的产氧光合作用
Photosystem I-independent oxygenic photosynthesis in cyanobacteria.
摘要(中文译文)
产氧光合作用通过从水到NADP的线性电子流生成ATP和NADPH，这一过程被认为需要光系统I（PSI）参与还原力形成。本文证明，在蓝细菌集胞藻PCC 6803中，产氧光合作用可在没有PSI的情况下运行。利用基因工程和适应性实验室进化，我们获得了能够进行光自养生长、无机碳固定和光驱动放氧的PSI缺陷谱系。不依赖PSI的光自养能力源于至少两种蛋白质的共突变，其中包括翻译延伸因子G（FusA），并且需要功能性的NDH-1复合体。我们提出，进化菌株中的光驱动电子传递被重组为两个分支：一个分支涉及末端氧化酶以产生质子动力势，第二个分支中反向NDH-1活性利用该梯度产生还原力。这些发现揭示了类囊体电子传递网络中意想不到的可塑性，并促使重新评估产氧光合作用中对PSI的经典需求。
Abstract
Oxygenic photosynthesis generates ATP and NADPH via linear electron flow from water to NADP, a process thought to require photosystem I (PSI) for reductant formation. Here we demonstrate that oxygenic photosynthesis can operate without PSI in the cyanobacterium Synechocystis sp. PCC 6803. Using genetic engineering and adaptive laboratory evolution, we obtained PSI-deficient lineages capable of photoautotrophic growth, inorganic carbon fixation, and light-driven oxygen evolution. PSI-independent photoautotrophy arose from co-mutations in at least two proteins, including the translation elongation factor G (FusA), and required a functional NDH-1 complex. We propose that the light-driven electron transport in the evolved strains is reorganised in two branches: one involving terminal oxidases to generate proton motive force, and a second in which reverse NDH-1 activity exploits this gradient to produce reductant. These findings uncover unexpected plasticity in the thylakoid electron transport network and prompt a reassessment of the canonical requirement for PSI in oxygenic photosynthesis.
如何引用
AMA
Marta Ludwiczak, Marcel Dann, Theo Figueroa-Gonzalez, Eslam M Abdel-Salam, Weiyang Chen, Serena Schwenkert, et al. Photosystem I-independent oxygenic photosynthesis in cyanobacteria.. Nature communications. 2026; doi:10.1038/s41467-026-74903-2.
APA
Marta Ludwiczak, Marcel Dann, Theo Figueroa-Gonzalez, Eslam M Abdel-Salam, Weiyang Chen, Serena Schwenkert, et al (2026). Photosystem I-independent oxygenic photosynthesis in cyanobacteria.. Nature communications. https://doi.org/10.1038/s41467-026-74903-2
GB/T 7714
Marta Ludwiczak, Marcel Dann, Theo Figueroa-Gonzalez, Eslam M Abdel-Salam, Weiyang Chen, Serena Schwenkert, et al. Photosystem I-independent oxygenic photosynthesis in cyanobacteria.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74903-2.
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