具有机械增强界面的统一双层膜用于阶段适应性骨再生
Unified bilayer membranes with mechanically reinforced interface for stage-adaptive bone regeneration.
摘要(中文译文)
引导骨再生使用双层膜时，常受限于界面整合不良、稳定性不足以及对复杂愈合过程调控适应性有限等问题。为此，我们开发了一种统一双层膜，通过同质异构原位构建策略，在屏障层与多孔层之间形成增强的双交联界面。其优异的机械强度（干态约156 MPa，湿态约8.6 MPa）确保了体内长期降解稳定性和生物活性。结合序贯释放的生物活性成分，我们证明该统一双层膜可调控抗菌功能、免疫调节、神经血管化和成骨作用，从而实现阶段适应性骨再生。我们在大鼠临界尺寸颅骨缺损模型中进行测试，证明该膜显著优于临床胶原膜，8周后骨体积再生约为后者的2.7倍。本研究进一步阐明了统一双层膜的时空成骨机制，其在阶段适应性骨修复过程中构建了良好整合的再生微环境，并促进了紧密互联的神经血管-成骨通讯网络。
Abstract
Guided bone regeneration using double-layered membranes is often hindered by poor interfacial integration, insufficient stability, and limited adaptability to regulate the complex healing process. Herein, we develop a unified bilayer membrane featuring a reinforced dual-crosslinking interface between barrier and porous layers through a homogeneous isomeric in-situ fabrication strategy. Its superior mechanical strength (~156 MPa in dry state and ~8.6 MPa in wet state) ensures the long-term degradation stability and bioactivity in vivo. Combined with sequentially released bioactive components, we show that the unified bilayer membrane regulates antibacterial functions, immunomodulation, neurovascularization, and osteogenesis, thereby enabling stage-adaptive bone regeneration. We test in a rat critical-sized cranial defect model to demonstrate that the membrane significantly outperforms the clinical collagen membrane, achieving ~2.7-fold greater bone volume regeneration after 8 weeks. This work further elucidates the spatiotemporal osteogenic mechanisms of the unified bilayer membrane, which constructs a well-integrated regenerative microenvironment and promotes a tightly interconnected neurovascular-osteogenic communication network during stage-adaptive bone repair.
如何引用
AMA
Yuhan Du, Yangyi Nie, Zhiheng Luo, Junjie Deng, Zili Xu, Yuyang Zhang, et al. Unified bilayer membranes with mechanically reinforced interface for stage-adaptive bone regeneration.. Nature communications. 2026; doi:10.1038/s41467-026-74932-x.
APA
Yuhan Du, Yangyi Nie, Zhiheng Luo, Junjie Deng, Zili Xu, Yuyang Zhang, et al (2026). Unified bilayer membranes with mechanically reinforced interface for stage-adaptive bone regeneration.. Nature communications. https://doi.org/10.1038/s41467-026-74932-x
GB/T 7714
Yuhan Du, Yangyi Nie, Zhiheng Luo, Junjie Deng, Zili Xu, Yuyang Zhang, et al. Unified bilayer membranes with mechanically reinforced interface for stage-adaptive bone regeneration.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74932-x.
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