临床肠球菌分离株中毒力决定因子（esp、cylA和gelE）的分布：来自印度中部的分子研究
Distribution of Virulence Determinants (esp, cylA, and gelE) Among Clinical Enterococcus Isolates: A Molecular Study From Central India.
摘要(中文译文)
肠球菌属包含革兰阳性球菌。在抗生素广泛使用之前，肠球菌是心内膜炎、尿路感染（UTIs）和腹腔内感染的已知病因。已发现的三种最重要的肠球菌毒力因子为溶细胞素（cylA）、肠球菌表面蛋白（esp）和明胶酶（gelE）。抗生素耐药基因数量的不断增加引发了人们对感染性疾病的严重担忧。本研究获得了机构伦理委员会的批准（MU/Research/EC/Ph.D./2021/58），每一份样本均采集自印度印多尔Index医学院医院与研究中心的住院患者。2021年12月至2024年8月期间共收集了106株肠球菌分离株，并对其菌种分布进行了分析。采用标准分子技术鉴定毒力基因，如溶细胞素（cylA）、肠球菌表面蛋白（esp）和明胶酶（gelE）。在通过逆转录聚合酶链反应（RT-PCR）分析的106株肠球菌分离株中，esp基因最为常见（74.53%），其次为cylA（55.66%）和gelE（45.28%）。与相比，显示出更高的毒力基因频率。对红霉素、青霉素、氯霉素和四环素观察到高水平耐药，而大多数分离株对利奈唑胺和万古霉素仍保持敏感。这些发现表明肠球菌分离株中存在毒力相关基因及多变的抗菌药物耐药模式。本研究揭示了肠球菌菌株具有显著的致病潜力，其主要毒力基因（esp、cylA和gelE）的高流行率即为证据。
Abstract
The genus Enterococcus contains gram-positive cocci. Before the widespread use of antibiotics, enterococci were known causes of endocarditis, urinary tract infections (UTIs), and intra-abdominal infections. The three most important enterococcal virulence agents discovered are cytolysin (cylA), enterococcal surface protein (esp), and gelatinase (gelE). Serious concerns about infectious illnesses are raised by the increasing number of genes for antibiotic resistance. The study was granted permission by the Institutional Ethical Committee (MU/Research/EC/Ph.D./2021/58), and every single sample was taken from patients who were hospitalized at the Index Medical College Hospital and Research Center in Indore, India. One hundred and six enterococcal isolates were collected between December 2021 and August 2024, and their species distribution was examined. Standard molecular techniques were used to identify virulence genes, such as cytolysin (cylA), enterococcal surface protein (esp), and gelatinase (gelE). Among 106 Enterococcus isolates analyzed by reverse transcription polymerase chain reaction (RT-PCR), the esp gene was the most prevalent (74.53%), followed by cylA (55.66%) and gelE (45.28%). showed higher frequencies of virulence genes compared to . High resistance was observed to erythromycin, penicillin, chloramphenicol, and tetracycline, whereas most isolates remained sensitive to linezolid and vancomycin. These findings indicate the presence of virulence-associated genes and variable antimicrobial resistance patterns among the Enterococcus isolates. The study reveals a significant pathogenic potential of Enterococcus strains, evidenced by the high prevalence of major virulence genes (esp, cylA, and gelE).
如何引用
AMA
Bhawani Shankar Verma, Prashanth K Guddeti, Ramanath Karicheri, Md Abdullah, Hemant B Patil. Distribution of Virulence Determinants (esp, cylA, and gelE) Among Clinical Enterococcus Isolates: A Molecular Study From Central India.. Cureus. 2026; doi:10.7759/cureus.110174.
APA
Bhawani Shankar Verma, Prashanth K Guddeti, Ramanath Karicheri, Md Abdullah, Hemant B Patil (2026). Distribution of Virulence Determinants (esp, cylA, and gelE) Among Clinical Enterococcus Isolates: A Molecular Study From Central India.. Cureus. https://doi.org/10.7759/cureus.110174
GB/T 7714
Bhawani Shankar Verma, Prashanth K Guddeti, Ramanath Karicheri, Md Abdullah, Hemant B Patil. Distribution of Virulence Determinants (esp, cylA, and gelE) Among Clinical Enterococcus Isolates: A Molecular Study From Central India.[J]. Cureus, 2026 doi:10.7759/cureus.110174.
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