连续的MAP激酶级联调节机械信号传导
A sequential MAP kinase cascade regulates mechanical signalling.
摘要(中文译文)
植物通过响应风、触碰或创伤等机械刺激来保障自身的发育与生存。促分裂原活化蛋白激酶（MAPK）可被机械刺激激活，这一现象早在26年前已有报道，但其上游调控机制及功能仍不清楚。我们发现，机械刺激可在60秒内激活MAPKKK3/4/5-MKK4/5-MPK3/6级联反应，进而诱导约800个基因的表达，涵盖大部分早期触碰反应。其中多数基因与受CAMTA1/2/3调控的触碰响应基因重叠，揭示了MAPK与CAMTA转录因子之间的相互作用。此外，MKK4/5缺失会导致触碰调控的蛋白质磷酸化整体受损，表明它们是关键的调控因子。相反，磷酸化并未明显影响触碰诱导的茉莉酸（JA）信号通路的早期激活，JA缺失也不影响整体早期触碰磷酸化蛋白质组。最后，MAPKKK3/4/5和MKK4/5缺失会减弱接触形态发生（thigmomorphogenesis），凸显了所鉴定的MAPK级联在胁迫条件下调控植物生长中的重要性。总之，我们现已鉴定出MAPKKK3/4/5-MKK4/5-MPK3/6级联是植物中关键的触碰和创伤信号通路。
Abstract
Plants respond to mechanical stimulations like wind, touching or wounding to safeguard their development and survival. Mitogen Activated Protein Kinases (MAPKs) activation by mechanostimuli was reported 26 years ago, but the upstream regulatory mechanism and function remained unknown. We report that mechanostimulation activates a MAPKKK3/4/5-MKK4/5-MPK3/6 cascade within 60 seconds, leading to induction of ~800 genes, encompassing most of the early touch response. Most genes overlap with touch-responsive genes regulated by CAMTA1/2/3, exposing an interplay between MAPKs and CAMTA transcription factors. Furthermore, loss of MKK4/5 leads to global impairment of touch-regulated protein phosphorylation, demonstrating they are crucial regulators. In contrast, phosphorylation does not clearly affect early activation of the touch-induced jasmonic acid (JA) signalling pathway, nor does loss of JA affect the overall early touch-phosphoproteome. Lastly, loss of MAPKKK3/4/5 and MKK4/5 reduces thigmomorphogenesis, underlining the importance of the identified MAPK cascade for steering plant growth during stress. In summary, we have now identified a MAPKKK3/4/5-MKK4/5-MPK3/6 cascade as a key touch- and wounding signalling pathway in plants.
如何引用
AMA
Huy Cuong Tran, Essam Darwish, Viktor Johansson, Guadalupe Fernandez-Milmanda, Tingting Zhu, Cássio Flávio Fonseca de Lima, et al. A sequential MAP kinase cascade regulates mechanical signalling.. Nature communications. 2026; doi:10.1038/s41467-026-74994-x.
APA
Huy Cuong Tran, Essam Darwish, Viktor Johansson, Guadalupe Fernandez-Milmanda, Tingting Zhu, Cássio Flávio Fonseca de Lima, et al (2026). A sequential MAP kinase cascade regulates mechanical signalling.. Nature communications. https://doi.org/10.1038/s41467-026-74994-x
GB/T 7714
Huy Cuong Tran, Essam Darwish, Viktor Johansson, Guadalupe Fernandez-Milmanda, Tingting Zhu, Cássio Flávio Fonseca de Lima, et al. A sequential MAP kinase cascade regulates mechanical signalling.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74994-x.
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