设计一个强大而自主的生物漏斗，用于木质素相关芳烃的有效稳定化
Engineering a robust and autonomous biological funneling for efficient valorization of lignin-related aromatics.
摘要(中文译文)
木质素相关芳香族化合物是有前景的可再生原料，但其微生物转化为有用化合物从根本上受限于底物毒性和代谢通量失衡。在此，我们报道了谷氨酸棒杆菌中的一种自主微生物系统，该系统将进化工程与多重动态控制相结合以克服这些限制。该系统具有一个稳健的进化底盘，其细胞防御能力增强且芳香族外排机制得到升级。我们设计了一个分层的多输入调控网络，能够实现对代谢途径的实时、底物响应性精准控制。该系统实现了多种木质素相关芳香族化合物向原儿茶酸的99.89%转化率，并随后达到53.40 g L⁻¹的滴度。这进一步支持了高滴度生产高附加值下游化学品，包括顺,顺-黏康酸和β-酮己二酸，滴度分别为51.90 g L⁻¹和38.33 g L⁻¹。这项工作为木质素原料自主生物加工为可持续高附加值产品建立了一个通用且可扩展的范式。
Abstract
Lignin-related aromatics are promising renewable feedstocks, but their microbial conversion into useful compounds is fundamentally constrained by substrate toxicity and metabolic flux imbalance. Here, we report an autonomous microbial system in Corynebacterium glutamicum, which integrates evolutionary engineering with multiplexed dynamic control to overcome these constraints. This system features a robust, evolved chassis with reinforced cellular defenses and upgraded aromatic efflux machinery. We engineer a layered, multi-input regulatory network that enables real-time, substrate-responsive and precise control of metabolic pathways. This system achieves 99.89% conversion of diverse lignin-related aromatics to protocatechuic acid and subsequently achieves a titer of 53.40 g L⁻¹. This further supports high-titer production of value-added downstream chemicals, including cis,cis-muconic acid and β-ketoadipic acid with titers of 51.90 g L⁻¹ and 38.33 g L⁻¹, respectively. This work establishes a versatile and scalable paradigm for the autonomous bioprocessing of lignin feedstocks into sustainable value-added products.
如何引用
AMA
Jianping Xu, Jian Zhang, Xiaotong Wang, Tengfei Zhao, Qingsheng Qi, Qian Wang. Engineering a robust and autonomous biological funneling for efficient valorization of lignin-related aromatics.. Nature communications. 2026; doi:10.1038/s41467-026-74424-y.
APA
Jianping Xu, Jian Zhang, Xiaotong Wang, Tengfei Zhao, Qingsheng Qi, Qian Wang (2026). Engineering a robust and autonomous biological funneling for efficient valorization of lignin-related aromatics.. Nature communications. https://doi.org/10.1038/s41467-026-74424-y
GB/T 7714
Jianping Xu, Jian Zhang, Xiaotong Wang, Tengfei Zhao, Qingsheng Qi, Qian Wang. Engineering a robust and autonomous biological funneling for efficient valorization of lignin-related aromatics.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74424-y.
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