人体器官N-糖蛋白组的全面概况
A comprehensive landscape of human organ N-glycoproteome.
摘要(中文译文)
N-糖基化作为一种结构复杂的常见翻译后修饰，在蛋白质折叠、细胞识别和信号通路中发挥关键作用。当前基于质谱的N-糖蛋白质组学技术已能够对人多种器官的N-糖蛋白质组进行深度表征。然而，涵盖人体多器官的全面N-糖蛋白质组图谱仍然缺乏。本研究构建了一个系统性的人体N-糖蛋白质组图谱，覆盖18个器官/组织的74例样本，在5062个N-糖蛋白的7543个N-糖基化位点上，鉴定到57,884条N-糖链结构水平的完整N-糖肽和23,863条单糖组成水平的完整N-糖肽。研究观察到组织特异性的N-糖基化模式以及唾液酸化与岩藻糖基化之间的交互作用。基于本研究的N-糖肽数据集，利用多种机器学习模型训练了一个器官分类器。该图谱辅以统一的多软件分析框架，为器官特异性糖生物学提供了新的见解，并为理解人体组织的生理性N-糖基化特征建立了基础性参考。
Abstract
N-glycosylation, as a common post-translational modification with complex structures, plays key roles in protein folding, cellular recognition and signaling pathways. State-of-the-art mass spectrometry-based N-glycoproteomics has enabled deep N-glycoproteome characterization of various human organs. However, a comprehensive N-glycoproteome landscape of human organs remains lacking. Here we present a systematic human N-glycoproteome atlas spanning 74 subjects across 18 organs/tissues with identification of 57,884 N-glycan structure-level and 23,863 monosaccharide composition-level intact N-glycopeptides on 7543 N-glycosites of 5062 N-glycoproteins. Tissue-specific N-glycosylation patterns and crosstalk between sialylation and fucosylation are observed. An organ classifier using multiple machine learning models on our N-glycopeptide dataset is trained. This atlas, complemented by a unified multi-software analysis framework, provides insights into organ-specific glycobiology and establishes a fundamental reference for understanding physiological N-glycosylation characteristics of human tissues.
如何引用
AMA
Xiaoyu Hu, Tao Wang, Chao Qin, Yanggang Yuan, Suideng Qin, Hongwei Liang, et al. A comprehensive landscape of human organ N-glycoproteome.. Nature communications. 2026; doi:10.1038/s41467-026-74631-7.
APA
Xiaoyu Hu, Tao Wang, Chao Qin, Yanggang Yuan, Suideng Qin, Hongwei Liang, et al (2026). A comprehensive landscape of human organ N-glycoproteome.. Nature communications. https://doi.org/10.1038/s41467-026-74631-7
GB/T 7714
Xiaoyu Hu, Tao Wang, Chao Qin, Yanggang Yuan, Suideng Qin, Hongwei Liang, et al. A comprehensive landscape of human organ N-glycoproteome.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74631-7.
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