dITP诱导的重塑激活了孔明抗噬菌体防御中的丝状效应复合物
dITP-induced remodeling activates the filamentous effector complex in Kongming anti-phage defense.
摘要(中文译文)
细菌中已鉴定出多种基于核苷酸信号传导的抗病毒免疫系统。其中之一是孔明系统（Kongming system），包含一个效应复合物（KomBC），由非典型嘌呤NTP焦磷酸酶（KomB）和含SIR2结构域的蛋白（KomC）组成。孔明系统由噬菌体感染时产生的一种非典型信号核苷酸dITP激活。本研究通过结构与功能分析表明，KomBC组装为螺旋丝状结构，由垂直堆叠的4：4 KomB-KomC重复单元构成。dITP与KomB的结合启动丝状结构内渐进性构象重排，最终将丝状结构重塑为一种独特架构，其中KomC采用具有NADase活性的活性构象。我们进一步鉴定出一个可能参与SIR2家族蛋白获得NADase活性的关键残基。因此，本研究结果阐明了KomBC的激活机制，揭示了一种由信号分子激活效应物的独特模式。
Abstract
Several antiviral immune systems based on nucleotide signaling have been identified in bacteria. One of them is the Kongming system, which includes an effector complex (KomBC), composed of a non-canonical purine NTP pyrophosphatase (KomB) and a SIR2 domain-containing protein (KomC). The Kongming system is activated by an atypical signaling nucleotide, dITP, generated upon phage infection. Here, we use structural and functional analyses to show that KomBC assembles into a helical filament, built from vertically stacked 4:4 KomB-KomC repeating units. Binding of dITP to KomB initiates progressive conformational rearrangements within the filament, ultimately remodeling the filament into a distinct architecture in which KomC adopts an active conformation with NADase activity. We further identify a key residue potentially involved in the acquisition of NADase activity in SIR2 family proteins. Thus, our results elucidate the activation mechanism of KomBC, revealing a distinct mode of effector activation by a signaling molecule.
如何引用
AMA
Xia Li, Min Liu, Wenxue Jiang, Guangbo Yan, Dujuan Shi, Qiong Xing, et al. dITP-induced remodeling activates the filamentous effector complex in Kongming anti-phage defense.. Nature communications. 2026; doi:10.1038/s41467-026-74710-9.
APA
Xia Li, Min Liu, Wenxue Jiang, Guangbo Yan, Dujuan Shi, Qiong Xing, et al (2026). dITP-induced remodeling activates the filamentous effector complex in Kongming anti-phage defense.. Nature communications. https://doi.org/10.1038/s41467-026-74710-9
GB/T 7714
Xia Li, Min Liu, Wenxue Jiang, Guangbo Yan, Dujuan Shi, Qiong Xing, et al. dITP-induced remodeling activates the filamentous effector complex in Kongming anti-phage defense.[J]. Nature communications, 2026 doi:10.1038/s41467-026-74710-9.
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