由两种改良的活病毒样毒株重组的新型猪繁殖与呼吸综合征病毒2株的出现及其对仔猪的致病性
Emergence of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 Strain Recombined from Two Modified Live Virus-like Strains and Its Pathogenicity for Piglets.
摘要(中文译文)
猪繁殖与呼吸综合征（PRRS）由猪繁殖与呼吸综合征病毒（PRRSV）引起，对全球养猪业构成严重威胁。尽管数十年来改良活病毒（MLV）疫苗已在田间广泛用于PRRS的预防，但这些疫苗的安全性和有效性长期存在争议。在此，我们报道了中国一种罕见的重组模式：出现了一株由两株MLV样毒株重组而成的新型NADC30样PRRSV毒株。基因组比较分析显示，SCMS2025分离株在NSP2蛋白中存在非连续的136个氨基酸缺失，且与谱系5（L5）毒株的核苷酸同源性最高，为87.6%。系统发育分析表明，基于ORF5基因分型，SCMS2025被归类为L1（NADC30样）毒株，而基于全基因组序列，其位于L1与L5毒株之间的独立分支。值得注意的是，基因组重组分析显示，该新出现的PRRSV分离株可能源于NADC30样毒株与两株MLV样毒株（RespPRRS MLV和TJbd14-1 MLV样毒株）之间的复杂重组事件。此外，SCMS2025感染引起短暂的明显临床症状，随后迅速康复，表明该新型PRRSV分离株为低致病性毒株。值得注意的是，所有接种SCMS2025的仔猪在整个14天观察期内PRRSV特异性抗体均保持血清阴性，提示宿主体液免疫应答启动延迟。本研究为PRRSV通过谱系间重组持续进化提供了证据，并凸显了研发安全有效疫苗的迫切需求。
Abstract
Porcine reproductive and respiratory syndrome (PRRS), caused by PRRS virus (PRRSV), poses a serious threat to the global swine industry. Although modified live virus (MLV) vaccines have been widely used in the field for PRRS prevention for decades, the safety and efficacy of these vaccines have long been controversial. Here, we report a rare recombination pattern in China: the emergence of a novel NADC30-like PRRSV strain recombined from two MLV-like strains. Genome comparative analysis reveals that the SCMS2025 isolate has a non-continuous 136-amino acid deletion in the NSP2 protein and shares the highest nucleotide identity of 87.6% with lineage 5 (L5) strains. Phylogenetic analysis showed that SCMS2025 was classified into L1 (NADC30-like) strains based on ORF5 genotyping, whereas it belonged to a single branch between L1 and L5 strains based on the complete genomic sequences. Strikingly, genomic recombination analysis revealed that the newly emerged PRRSV isolate likely resulted from complex recombination events between NADC30-like and two MLV-like strains (RespPRRS MLV and TJbd14-1 MLV-like strains). Furthermore, SCMS2025 infection caused transient overt clinical signs followed by rapid recovery, indicating that the novel PRRSV isolate is a low pathogenic strain. Notably, all SCMS2025-inoculated piglets remained seronegative for PRRSV-specific antibodies throughout the entire 14-day observation period, suggesting a delayed onset of the host humoral immune response. Our study provides evidence for the ongoing evolution of PRRSV through inter lineage recombination and highlights the urgent need for safe and effective vaccines.
如何引用
AMA
Yiwen Pei, Xue Gao, Shuo Feng, Danjiao Yang, Runmin Kang, Jifeng Yu, et al. Emergence of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 Strain Recombined from Two Modified Live Virus-like Strains and Its Pathogenicity for Piglets.. Animals : an open access journal from MDPI. 2026; doi:10.3390/ani16121903.
APA
Yiwen Pei, Xue Gao, Shuo Feng, Danjiao Yang, Runmin Kang, Jifeng Yu, et al (2026). Emergence of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 Strain Recombined from Two Modified Live Virus-like Strains and Its Pathogenicity for Piglets.. Animals : an open access journal from MDPI. https://doi.org/10.3390/ani16121903
GB/T 7714
Yiwen Pei, Xue Gao, Shuo Feng, Danjiao Yang, Runmin Kang, Jifeng Yu, et al. Emergence of a Novel Porcine Reproductive and Respiratory Syndrome Virus 2 Strain Recombined from Two Modified Live Virus-like Strains and Its Pathogenicity for Piglets.[J]. Animals : an open access journal from MDPI, 2026 doi:10.3390/ani16121903.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出