过渡期棕色瑞士奶牛和荷斯坦奶牛循环白细胞中的基因表达
Gene Expression in Circulating Leukocytes in Brown Swiss and Holstein Cows During the Transition Period.
摘要(中文译文)
过渡期以显著的代谢和免疫变化为特征，这些变化增加了奶牛对疾病的易感性。遗传背景可能影响该阶段免疫反应的调控。本研究旨在比较褐牛和荷斯坦牛过渡期循环白细胞中免疫相关基因的表达。22头妊娠青年母牛（10头褐牛和12头荷斯坦牛）在相同条件下饲养，并分别于产犊前21天、产犊后7天和产犊后28天采样。使用PAXgene管采集全血，并通过定量PCR分析基因表达。目标基因与免疫识别、白细胞迁移和黏附、抗菌活性、氧化应激及炎症反应相关。两个品种的基因表达谱随时间变化存在差异。荷斯坦牛在产犊前显示参与病原体识别的基因表达较高，包括 和 。相比之下，褐牛显示与白细胞迁移（ 、 ）、抗菌活性（ 、 ）、炎症反应（ 、 ）及氧化应激（ 、 ）相关的基因表达较高，尤其是在产犊后。这些结果表明白细胞基因表达存在品种相关差异，并提示遗传背景影响过渡期的免疫适应。
Abstract
The transition period is characterized by major metabolic and immunological changes that increase disease susceptibility in dairy cows. Genetic background may influence the regulation of immune responses during this phase. The aim of this study was to compare the expression of immune-related genes in circulating leukocytes of Brown Swiss and Holstein cows across the transition period. Twenty-two pregnant heifers (10 Brown Swiss and 12 Holstein) were managed under identical conditions and sampled at -21, +7, and +28 days relative to calving. Whole blood was collected using PAXgene tubes, and gene expression was analyzed by quantitative PCR. Target genes were associated with immune recognition, leukocyte migration and adhesion, antimicrobial activity, oxidative stress, and inflammatory response. Gene expression profiles differed between breeds over time. Holstein cows showed higher expression of genes involved in pathogen recognition before calving, including and . In contrast, Brown Swiss cows showed higher expression of genes related to leukocyte migration (, ), antimicrobial activity (, ), inflammatory response (, ), and oxidative stress (, ), particularly after calving. These results indicate breed-related differences in leukocyte gene expression and suggest that genetic background influences immune adaptation during the transition period.
如何引用
AMA
Marta Sfulcini, Vincenzo Lopreiato, Fiorenzo Piccioli-Cappelli, Luca Cattaneo, Matteo Mezzetti, Alessandro Catellani, et al. Gene Expression in Circulating Leukocytes in Brown Swiss and Holstein Cows During the Transition Period.. Animals : an open access journal from MDPI. 2026; doi:10.3390/ani16121858.
APA
Marta Sfulcini, Vincenzo Lopreiato, Fiorenzo Piccioli-Cappelli, Luca Cattaneo, Matteo Mezzetti, Alessandro Catellani, et al (2026). Gene Expression in Circulating Leukocytes in Brown Swiss and Holstein Cows During the Transition Period.. Animals : an open access journal from MDPI. https://doi.org/10.3390/ani16121858
GB/T 7714
Marta Sfulcini, Vincenzo Lopreiato, Fiorenzo Piccioli-Cappelli, Luca Cattaneo, Matteo Mezzetti, Alessandro Catellani, et al. Gene Expression in Circulating Leukocytes in Brown Swiss and Holstein Cows During the Transition Period.[J]. Animals : an open access journal from MDPI, 2026 doi:10.3390/ani16121858.
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