多组织代谢组学揭示了与产蛋母鸡腹部脂肪和蛋黄之间的脂质分配相关的代谢特征
Multi-Tissue Metabolomics Reveals Metabolic Signatures Associated with Lipid Partitioning Between Abdominal Fat and Egg Yolk in Laying Hens.
摘要(中文译文)
蛋鸡脂肪沉积涉及多个组织中的脂质合成、转运、储存与分配，但腹部脂肪（AF）与蛋黄（EY）脂质沉积之间的代谢联系仍不清楚。本研究整合了248只100周龄纯种罗得岛红母鸡的全基因组重测序数据与来自肝脏、十二指肠、回肠、盲肠和血清的非靶向代谢组学图谱。我们估计了代谢物对22个脂肪沉积相关性状的解释方差（），评估了代谢物遗传力，并结合Spearman相关分析与双向广义汇总数据孟德尔随机化（GSMR）来识别与AF和EY性状相关的共享代谢物及通路。结果显示明显的组织特异性，肝脏、血清和十二指肠对77.3%（17/22）、72.7%（16/22）和68.2%（15/22）的脂肪相关性状表现出显著解释信号。肝脏、AF和体重相关性状表现出更强的代谢组学解释信号，各组织中的显著比例分别为71.4-100.0%、28.6-100.0%和100.0%，而EY相关性状则表现出更受限且具有组织特异性的关联（0-33.3%）。相关分析识别出肝脏富集的AF-EY共享代谢物（如NADPH、硫酸胆固醇、N6,N6,N6-三甲基赖氨酸），其中多数在AF与EY相关性状之间表现出相反的关联模式。双向GSMR优先筛选出20种对AF和EY性状具有相反推定效应的候选代谢物，包括CDP-胆碱、磷酸胆碱和尿囊素。通路整合突出显示果糖/甘露糖代谢、甘油磷脂代谢、ABC转运蛋白、叶酸/一碳代谢、氨基酸代谢和能量代谢是AF-EY共享网络的核心组成部分。这些发现揭示了脂肪沉积的组织特异性与共享代谢基础，并识别出与蛋鸡腹部脂肪和蛋黄之间脂质分配相关的候选代谢特征。
Abstract
Fat deposition in laying hens involves lipid synthesis, transport, storage, and allocation across multiple tissues, yet the metabolic links between abdominal fat (AF) and egg yolk (EY) lipid deposition remain unclear. Here, we integrated whole-genome resequencing data with untargeted metabolomic profiles from the liver, duodenum, ileum, cecum, and serum of 248 purebred Rhode Island Red hens at 100 weeks of age. We estimated metabolite-explained variance () for 22 fat deposition-related traits, evaluated metabolite heritability, and combined Spearman correlation analysis with bidirectional generalized summary-data-based Mendelian randomization (GSMR) to identify shared metabolites and pathways associated with AF and EY traits. The showed clear tissue specificity, with the liver, serum, and duodenum showing significant explanatory signals for 77.3% (17/22), 72.7% (16/22), and 68.2% (15/22) of fat-related traits. Liver-, AF-, and body weight-related traits showed stronger metabolomic explanatory signals, with significant proportions of 71.4-100.0%, 28.6-100.0%, and 100.0% across tissues, respectively, whereas EY-related traits showed more restricted and tissue-specific associations (0-33.3%). Correlation analysis identified liver-enriched AF-EY shared metabolites (e.g., NADPH, cholesteryl sulfate, N6,N6,N6-trimethyllysine), most of which showed opposite association patterns between AF- and EY-related traits. Bidirectional GSMR prioritized 20 candidate metabolites with opposite putative effects on AF and EY traits, including CDP-choline, phosphorylcholine, and allantoin. Pathway integration highlighted fructose/mannose metabolism, glycerophospholipid metabolism, ABC transporters, folate/one-carbon metabolism, amino acid metabolism, and energy metabolism as core components of the AF-EY shared network. These findings reveal tissue-specific and shared metabolic bases of fat deposition and identify candidate metabolic signatures associated with lipid partitioning between abdominal fat and egg yolk in laying hens.
如何引用
AMA
Wenxin Zhang, Ronglang Cai, Fangren Lan, Guiqin Wu, Guangqi Li, Yiyuan Yan, et al. Multi-Tissue Metabolomics Reveals Metabolic Signatures Associated with Lipid Partitioning Between Abdominal Fat and Egg Yolk in Laying Hens.. Animals : an open access journal from MDPI. 2026; doi:10.3390/ani16121812.
APA
Wenxin Zhang, Ronglang Cai, Fangren Lan, Guiqin Wu, Guangqi Li, Yiyuan Yan, et al (2026). Multi-Tissue Metabolomics Reveals Metabolic Signatures Associated with Lipid Partitioning Between Abdominal Fat and Egg Yolk in Laying Hens.. Animals : an open access journal from MDPI. https://doi.org/10.3390/ani16121812
GB/T 7714
Wenxin Zhang, Ronglang Cai, Fangren Lan, Guiqin Wu, Guangqi Li, Yiyuan Yan, et al. Multi-Tissue Metabolomics Reveals Metabolic Signatures Associated with Lipid Partitioning Between Abdominal Fat and Egg Yolk in Laying Hens.[J]. Animals : an open access journal from MDPI, 2026 doi:10.3390/ani16121812.
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