肠道微生物群改变病毒性心肌炎小鼠的心脏代谢和免疫系统组成
Gut microbiota alters cardiac metabolism and immune system composition in viral myocarditis mice.
摘要(中文译文)
肠道菌群对宿主生理调节的影响近年来受到广泛关注，尤其是在代谢和免疫系统方面。然而，肠道菌群在病毒性心肌炎（VMC）中的作用仍有待充分阐明。采用CVB3注射Balb/c小鼠诱导VMC。VMC小鼠接受粪菌移植（FMT）或抗生素（ABX）治疗，以评估这些干预措施的治疗效果。采用超声心动图、HE染色和Masson染色评估心脏功能和病理变化。通过16S rDNA测序探究肠道微生物组成的变化。采用基于UPLC-MS/MS的代谢组学检测心脏代谢谱的紊乱。应用流式细胞术分析免疫细胞亚群的动态变化，包括M1、M2、Th1、Th2、Th17和Treg细胞。采用RT-PCR定量检测心脏中细胞因子的表达水平。FMT减轻了心脏炎症和纤维化，增强了心脏功能，重塑了VMC中肠道菌群的结构，并增加了细菌多样性，其中p-Proteobacteria富集，g-Pseudomonas、g-Streptococcus和g-Ralstonia减少。同时，FMT诱导了VMC中心脏代谢物的改变，类固醇激素生物合成通路富集。脱氧皮质酮、皮质酮、21-脱氧皮质醇和皮质醇酮与p_Spirochaetota和p_Kapabacteria呈显著负相关。此外，FMT降低了M1巨噬细胞、Th1和Th17细胞的比例，以及细胞因子TNF-a、IL-6和IL-1β的水平，并增加了M2巨噬细胞和Treg细胞。关于抗生素在VMC中的作用，我们的研究结果表明抗生素改变了肠道菌群、心肌代谢和免疫反应。与抗生素相比，FMT在减轻心脏炎症和纤维化方面效果更佳。在VMC小鼠中，肠道菌群介导了心脏代谢物和宿主免疫反应的紊乱，可能在心脏炎症和纤维化的发生发展中发挥重要作用。此外，FMT可能代表一种有前景的VMC治疗方法。
Abstract
The effects of the gut microbiota on the regulation of host physiology have recently garnered considerable attention, particularly in metabolism and the immune system. However, the role of the gut microbiota in viral myocarditis (VMC) remains to be fully elucidated. Balb/c mice were injected with CVB3 to induce VMC. VMC mice were treated with fecal microbiota transplantation (FMT) or antibiotics (ABX) to evaluate the therapeutic effects of these interventions. Echocardiography, HE, and Masson's staining of the heart were used to assess cardiac function and pathological changes. 16S rDNA sequencing was conducted to explore alterations in gut microbial composition. UPLC-MS/MS-based metabolomics was used to detect disturbances of cardiac metabolic profiles. Flow cytometry was applied to analyze the dynamics of immune cell subsets, including M1, M2, Th1, Th2, Th17, and Treg cells. RT-PCR was performed to quantify cytokine expression levels in the heart. FMT reduced cardiac inflammation and fibrosis, enhanced heart function, remodeled the structure of gut microbiota in VMC, and increased bacterial diversity, with an enrichment of p-Proteobacteria, the reduction of g-Pseudomonas, g-Streptococcus, and g-Ralstonia. Meanwhile, FMT induced alterations in cardiac metabolites in VMC, with enrichment of the steroid hormone biosynthesis pathway. A significant negative correlation was found between desoxycortone, corticosterone, 21-deoxycortisol, and cortodoxone with p_Spirochaetota and p_Kapabacteria. Furthermore, FMT reduced the proportions of M1 macrophages, Th1, and Th17 cells, as well as the cytokines TNF-a, IL-6, and IL-1β, and increased M2 macrophages and Treg cells. Regarding the role of antibiotics in VMC, our findings indicated that antibiotics altered the gut microbiota, myocardial metabolism, and immune response. Compared with antibiotics, FMT exerted a better effect on the alleviation of cardiac inflammation and fibrosis. In VMC mice, the gut microbiota, which mediates disturbances in cardiac metabolites and the host immune response, may contribute significantly to the development of cardiac inflammation and fibrosis. Furthermore, FMT may represent a promising therapeutic approach for VMC.
如何引用
AMA
Lili Chen, Weifeng Wu, Qing Kong. Gut microbiota alters cardiac metabolism and immune system composition in viral myocarditis mice.. Frontiers in microbiology. 2026; doi:10.3389/fmicb.2026.1828423.
APA
Lili Chen, Weifeng Wu, Qing Kong (2026). Gut microbiota alters cardiac metabolism and immune system composition in viral myocarditis mice.. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2026.1828423
GB/T 7714
Lili Chen, Weifeng Wu, Qing Kong. Gut microbiota alters cardiac metabolism and immune system composition in viral myocarditis mice.[J]. Frontiers in microbiology, 2026 doi:10.3389/fmicb.2026.1828423.
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