{"_":"益生菌对重金属的解毒作用：在模拟肠道和模型中的功能评估。","i":["离体"]}
{"_":"Probiotic detoxification of heavy metals: functional assessment in simulated intestinal and models.","i":["ex vivo"]}
摘要(中文译文)
生物修复是一种新兴的可持续策略，依赖微生物过程对环境污染物的解毒作用。在这些污染物中，重金属（HMs）是普遍存在、不可降解的有毒元素，对人类健康构成严重风险。本研究以单一菌株产品策略为出发点，分别评估了三株益生乳酸杆菌——LP14、LCR04和LA12——在胃肠道（GI）中对镉、铬、汞和铅的解毒能力，以及其减轻重金属诱导的肠道损伤的能力。在初步筛选出具有解毒潜力的菌株后，我们采用动态人体肠道微生物生态系统模拟器（SHIME®）模型，在连续的胃、小肠和结肠条件下评估了每株菌的存活、生长和重金属解毒能力。重金属螯合能力呈现出菌株特异性和金属特异性的差异：LP14和LCR04表现出较强的持久定植能力，并显著降低了重金属的生物利用度，而LA12在胃肠道模拟条件下显示出极低的解毒能力。在机制层面，只有在结肠阶段能够增殖的菌株才实现了实质性的重金属去除，凸显了代谢活性的重要性。在肠道系统（GEVS）中，单独暴露于重金属会破坏上皮屏障完整性并引发促炎反应。将重金属与各益生菌菌株预孵育后，这些效应显著减轻，肠道通透性和细胞因子谱得以恢复。总体而言，这些结果展示了一种新型的基于益生菌的肠道生物修复策略，并凸显了靶向单一菌株益生菌干预的转化潜力，可减少重金属暴露以保护肠道健康。
Abstract
Bioremediation is an emerging, sustainable strategy that relies on microbial processes to detoxify environmental pollutants. Among these pollutants, heavy metals (HMs) are pervasive, non-degradable toxic elements that pose serious risks to human health. In this study, we individually evaluated three probiotic lactobacilli strains- LP14, LCR04, and LA12-for their capacity to detoxify cadmium, chromium, mercury, and lead in the gastrointestinal (GI) tract, as well as their capacity to mitigate heavy metal-induced intestinal damage, with a single-strain product strategy in mind. After initial selection of the strains for their detoxifying potential, we employed a dynamic Simulator of the Human Intestinal Microbial Ecosystem (SHIME®) model and each strain's survival, growth, and heavy metal detoxification capacity was assessed under sequential gastric, small-intestinal, and colonic conditions. Strain- and metal-specific differences in HMs sequestration emerged: LP14 and LCR04 showed robust persistence and significantly reduced HMs bioavailability, whereas LA12 displayed minimal detoxification under GI simulation. Mechanistically, only the strains that proliferated in the colonic phase achieved substantial HM removal, underscoring the importance of metabolic activity . In a gut system (GEVS), heavy metal exposure alone compromised epithelial barrier integrity and triggered pro-inflammatory responses. Pre-incubation of the HMs with each of the probiotic strains markedly alleviated these effects, restoring intestinal permeability and cytokine profiles. Overall, the results demonstrate a novel probiotic-based intestinal bioremediation strategy and highlight the translational potential of targeted single-strain probiotic interventions, reducing heavy metal exposure to protect gut health.
如何引用
AMA
Marco Pane, Annachiara De Prisco, Angela Amoruso, Giovanni Deusebio, Massimo Marzorati, Marco Corazzari, et al. {"_":"Probiotic detoxification of heavy metals: functional assessment in simulated intestinal and models.","i":["ex vivo"]}. Frontiers in microbiology. 2026; doi:10.3389/fmicb.2026.1821114.
APA
Marco Pane, Annachiara De Prisco, Angela Amoruso, Giovanni Deusebio, Massimo Marzorati, Marco Corazzari, et al (2026). {"_":"Probiotic detoxification of heavy metals: functional assessment in simulated intestinal and models.","i":["ex vivo"]}. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2026.1821114
GB/T 7714
Marco Pane, Annachiara De Prisco, Angela Amoruso, Giovanni Deusebio, Massimo Marzorati, Marco Corazzari, et al. {"_":"Probiotic detoxification of heavy metals: functional assessment in simulated intestinal and models.","i":["ex vivo"]}[J]. Frontiers in microbiology, 2026 doi:10.3389/fmicb.2026.1821114.
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