猪肠道环路，探索氨基酸对回肠宿主微生物群串扰的影响
Porcine gut loops to explore the impact of amino acids on the host-microbiota crosstalk in the ileum.
摘要(中文译文)
氨基酸（AAs）对猪的生长、发育和健康至关重要，然而尽管关于黏膜屏障完整性的研究已十分广泛，氨基酸对肠道屏障和微生物群的直接影响仍知之甚少。虽然肠道细胞系和类器官提供了有价值的见解，但它们无法完全再现肠道黏膜的复杂性——在肠道黏膜中，多种细胞类型彼此之间以及与微生物群之间发生动态相互作用。为弥补这一不足，我们假设结扎肠环模型可作为中间体系，在减少实验动物需求的同时阐明氨基酸对肠道黏膜和微生物群的影响。本研究旨在首次检验该手术方法，评估氨基酸给药的短期影响，并为未来模型的优化奠定基础。我们在四头断奶仔猪中制备了十个回肠环，分别给予PBS、赖氨酸（3 g/L）、支链氨基酸混合物（3 g/L）或色氨酸（3 g/L），作用48小时。随后对肠环内容物代谢物、微生物群组成及黏膜基因表达的分析显示，基因表达、代谢和微生物谱主要受猪个体间差异驱动。尽管各处理组之间未检测到统计学显著差异，但识别出若干趋势。结扎肠环模型在短期评估氨基酸混合物对肠道黏膜和微生物群的影响方面显示出潜力，但尚需进一步优化以充分发挥其潜力。
Abstract
Amino acids (AAs) are essential for the growth, development, and health of pigs, yet their direct effects on the intestinal barrier and microbiota remain poorly understood , despite extensive studies on mucosal barrier integrity. While intestinal cell lines and organoids provide valuable insights, they cannot fully capture the complexity of the intestinal mucosa, where diverse cell types interact dynamically with each other and the microbiota. To address this gap, we hypothesized that the ligated intestinal loop model could serve as an intermediate system, clarifying the effects of AAs on the intestinal mucosa and microbiota while reducing the need for experimental animals. This study aimed to test this surgical approach for the first time, assessing the short-term impact of AA administration and laying the groundwork for future model optimization. We prepared ten ileal loops in four weaned pigs and administered either PBS, lysine (3 g/L), a mixture of branched-chain AAs (3 g/L), or tryptophan (3 g/L) for 48 h. Subsequent analyses of loop content metabolites, microbiota composition, and mucosal gene expression revealed that gene expression, metabolic, and microbial profiles were primarily driven by interindividual variability among pigs. Although no statistically significant differences were detected between treatment groups, several trends were identified. While the intestinal loop model shows potential for short-term assessments of AA mixtures on the intestinal mucosa and microbiota, further optimization is needed to fully realize its potential.
如何引用
AMA
Fanny Renois, Caroline Hervet, Céline Barc, Nathalie Kasal-Hoc, Elisabeth Jones, Charlie Roux, et al. Porcine gut loops to explore the impact of amino acids on the host-microbiota crosstalk in the ileum.. Frontiers in microbiology. 2026; doi:10.3389/fmicb.2026.1854850.
APA
Fanny Renois, Caroline Hervet, Céline Barc, Nathalie Kasal-Hoc, Elisabeth Jones, Charlie Roux, et al (2026). Porcine gut loops to explore the impact of amino acids on the host-microbiota crosstalk in the ileum.. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2026.1854850
GB/T 7714
Fanny Renois, Caroline Hervet, Céline Barc, Nathalie Kasal-Hoc, Elisabeth Jones, Charlie Roux, et al. Porcine gut loops to explore the impact of amino acids on the host-microbiota crosstalk in the ileum.[J]. Frontiers in microbiology, 2026 doi:10.3389/fmicb.2026.1854850.
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