IL-4介导的单核细胞分化调节CD163表达和PRRSV感染
IL-4-mediated monocyte differentiation modulates CD163 expression and PRRSV infection.
摘要(中文译文)
宿主免疫状态对病毒感染易感性具有关键影响；然而，细胞因子驱动的固有免疫分化对病毒容许性的影响仍知之甚少。本研究探讨了IL-4介导的单核细胞分化是否调节猪繁殖与呼吸综合征病毒（PRRSV）感染。猪单核细胞在含或不含IL-4的条件下经GM-CSF诱导分化，生成单核细胞衍生细胞（MDCs）。值得注意的是，与含IL-4分化生成的MDCs相比，不含IL-4生成的MDCs中PRRSV复制显著升高，且与CD163表达增加密切相关。一致地，CD163表达以IL-4依赖的方式下调。PRRSV感染后，不含IL-4分化的MDCs表现出TNF-产生减少，但IL-6和IL-10水平升高。此外，与含IL-4分化的MDCs相比，不含IL-4生成的MDCs中CD80和SLA-1表达增加，并显著抑制自体CD8 T细胞的增殖。分析进一步揭示，PRRSV感染猪肺泡MDCs中血浆IL-4水平与CD163表达呈负相关，且与CD8 T细胞比例增加相关。综上所述，这些发现表明IL-4介导的固有免疫编程调节细胞对PRRSV感染的容许性，并可能有助于保护性宿主免疫应答。
Abstract
Host immune status critically influences susceptibility to viral infections; however, the impact of cytokine-driven innate immune differentiation on viral permissiveness remains poorly understood. Here, we investigated whether IL-4-mediated monocyte differentiation modulates porcine reproductive and respiratory syndrome virus (PRRSV) infection. Porcine monocytes were differentiated in the presence of GM-CSF with or without IL-4 to generate monocyte-derived cells (MDCs). Notably, PRRSV replication was significantly higher in MDCs generated without IL-4 compared to those differentiated in the presence of IL-4, which was strongly associated with increased CD163 expression. Consistently, CD163 expression was downregulated in an IL-4-dependent manner. Following PRRSV infection, MDCs differentiated without IL-4 exhibited reduced TNF- production but elevated IL-6 and IL-10 levels. In addition, MDCs generated without IL-4 showed increased expression of CD80 and SLA-1 and significantly impaired the proliferation of autologous CD8 T cells compared to MDCs differentiated with IL-4. analysis further revealed a negative correlation between plasma IL-4 levels and CD163 expression in alveolar MDCs of PRRSV-infected pigs, which was associated with increased proportions of CD8 T cells. Collectively, these findings suggest that IL-4-mediated innate immune programming regulates cellular permissiveness to PRRSV infection and may contribute to protective host immune responses.
如何引用
AMA
Narae Kim, Young-Jun Ju, Yoon-Chul Kye, Cheol Gyun Kim, Young Jin Pyung, Da-Jeong Park, et al. IL-4-mediated monocyte differentiation modulates CD163 expression and PRRSV infection.. Frontiers in microbiology. 2026; doi:10.3389/fmicb.2026.1817131.
APA
Narae Kim, Young-Jun Ju, Yoon-Chul Kye, Cheol Gyun Kim, Young Jin Pyung, Da-Jeong Park, et al (2026). IL-4-mediated monocyte differentiation modulates CD163 expression and PRRSV infection.. Frontiers in microbiology. https://doi.org/10.3389/fmicb.2026.1817131
GB/T 7714
Narae Kim, Young-Jun Ju, Yoon-Chul Kye, Cheol Gyun Kim, Young Jin Pyung, Da-Jeong Park, et al. IL-4-mediated monocyte differentiation modulates CD163 expression and PRRSV infection.[J]. Frontiers in microbiology, 2026 doi:10.3389/fmicb.2026.1817131.
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