3D打印功能性工程肠模型
3D-printed functional engineered intestinal models.
摘要(中文译文)
肠道是维持人体生理稳态的重要器官，承担营养吸收、免疫监视和屏障完整性等关键功能。传统肠道模型，如二维单层培养和静态类器官，缺乏天然组织的复杂结构、多层细胞组织以及动态物理化学微环境，导致其对药物反应和疾病机制的预测能力较差。然而，三维生物打印技术的快速发展正在改变这一格局。构建能够忠实复现天然组织架构、细胞多样性和微环境信号的仿生肠道模型，已成为临床前研究的核心焦点。本文系统综述了三维打印仿生肠道模型的最新研究进展，深入探讨了生物打印技术、生物墨水配方、细胞来源、结构制造策略以及对功能性仿生的探索。我们还探讨了其与器官芯片系统的整合，以及在药物筛选、肠道发育、疾病研究和组织修复中日益广泛的应用。讨论的核心在于新技术飞跃——如新型生物墨水和创新打印方法——如何使复杂肠道结构的重建成为可能。尽管取得了显著进展，但在实现完全生理成熟度、可扩展性、标准化和成本效益方面仍存在障碍。最后，我们讨论了旨在提高模型保真度、实现多器官整合和增强其转化潜力的未来研究方向。
Abstract
The intestine is a vital organ, orchestrating nutrient absorption, immune surveillance, and barrier integrity functions essential for human physiological homeostasis. Traditional intestinal models, such as 2D monolayers and static organoids, lack the complex architecture, multi-layer cellular organization, and dynamic physicochemical microenvironment of native tissue, leading to poor predictive power for drug response and disease mechanisms. The rapid evolution of 3D bioprinting, however, is changing this landscape. It has become a central focus in preclinical research to construct biomimetic intestinal models that faithfully replicate native tissue architecture, cellular diversity, and microenvironmental cues. This review systematically summarizes the latest research progress in 3D-printed biomimetic intestinal models. We delve into bioprinting techniques, bioink formulation, cell sources, structural fabrication strategies, and the quest for functional biomimicry. We also explore their integration with organ-on-a-chip systems and their expanding applications in drug screening, intestinal development, diseases and tissue repair. Central to our discussion is how technological leaps, like novel bioinks and innovative printing methods, are enabling the reconstruction of complex gut architectures. Despite remarkable progress, hurdles remain in achieving full physiological maturity, scalability, standardization, and cost-effectiveness. Finally, we discuss future research directions aimed at enhancing model fidelity, enabling multi-organ integration, and strengthening their translational potential.
如何引用
AMA
Jin Xue, Qiancheng Jiao, Renjun Pei, Jie Huang, Xiaoyun Liu. 3D-printed functional engineered intestinal models.. Journal of materials chemistry. B. 2026; doi:10.1039/d6tb00574h.
APA
Jin Xue, Qiancheng Jiao, Renjun Pei, Jie Huang, Xiaoyun Liu (2026). 3D-printed functional engineered intestinal models.. Journal of materials chemistry. B. https://doi.org/10.1039/d6tb00574h
GB/T 7714
Jin Xue, Qiancheng Jiao, Renjun Pei, Jie Huang, Xiaoyun Liu. 3D-printed functional engineered intestinal models.[J]. Journal of materials chemistry. B, 2026 doi:10.1039/d6tb00574h.
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