{"_":"真菌γ-蛇麻烯合酶的表征。","i":["埋粪盘菌"]}
{"_":"Characterization of a γ-Humulene Synthase from Fungus .","i":["Ascobolus immersus"]}
摘要(中文译文)
γ-蛇麻烯是一种广泛分布于植物中的11元大环倍半萜，因其多样的生物活性而备受重视。然而，迄今为止尚未鉴定出专用的γ-蛇麻烯合酶。本研究鉴定并功能表征了来自真菌的倍半萜合酶（AiSTS）。异源表达和酶学实验表明，AiSTS特异性催化（E,E）-法尼基焦磷酸（(E,E)-FPP）转化为γ-蛇麻烯。此外，AiSTS还表现出显著的底物宽泛性，可接受（E）-香叶基焦磷酸（(E)-GPP）和（E,E,E）-香叶基香叶基焦磷酸（(E,E,E)-GGPP）生成多种单萜和二萜产物。定点突变揭示了七个对酶折叠、催化效率和产物选择性至关重要的氨基酸残基。AiSTS的发现拓展了真菌萜类合酶的多样性，为酶工程和γ-蛇麻烯的可持续微生物生产奠定了坚实基础。
Abstract
γ-Humulene is an 11-membered macrocyclic sesquiterpene widely distributed in plants and valued for its diverse biological activities. However, no dedicated γ-humulene synthase has been identified to date. In this study, a sesquiterpene synthase (AiSTS) from the fungus was identified and functionally characterized. heterologous expression and enzymatic assays demonstrated that AiSTS specifically catalyzes the conversion of (,)- farnesyl pyrophosphate ((,)-FPP) into γ-humulene. Furthermore, AiSTS also exhibits notable substrate promiscuity, accepting ()geranyl pyrophosphate (()GPP) and (,,)-geranylgeranyl pyrophosphate ((,,)-GGPP) to generate diverse mono- and diterpene products. Site-directed mutagenesis revealed seven amino acid residues that are essential for enzyme folding, catalytic efficiency, and product selectivity. The discovery of AiSTS expands the repertoire of fungal terpene synthases and lays a solid foundation for enzyme engineering and sustainable microbial production of γ-humulene.
如何引用
AMA
Haichun Zeng, Jiatong Zeng, Yuxin Zhou, Beilin Meng, Li Rao. {"_":"Characterization of a γ-Humulene Synthase from Fungus .","i":["Ascobolus immersus"]}. Journal of agricultural and food chemistry. 2026; doi:10.1021/acs.jafc.6c03126.
APA
Haichun Zeng, Jiatong Zeng, Yuxin Zhou, Beilin Meng, Li Rao (2026). {"_":"Characterization of a γ-Humulene Synthase from Fungus .","i":["Ascobolus immersus"]}. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.6c03126
GB/T 7714
Haichun Zeng, Jiatong Zeng, Yuxin Zhou, Beilin Meng, Li Rao. {"_":"Characterization of a γ-Humulene Synthase from Fungus .","i":["Ascobolus immersus"]}[J]. Journal of agricultural and food chemistry, 2026 doi:10.1021/acs.jafc.6c03126.
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