{"_":"RNAi介导的绒毛膜过氧化物酶功能表征：作为可持续控制草地贪夜蛾的双重影响靶标","i":["草地贪夜蛾"]}
{"_":"RNAi-Mediated Functional Characterization of Chorion Peroxidase as a Dual-Impact Target for Sustainable Control of .","i":["Spodoptera frugiperda"]}
摘要(中文译文)
绒毛膜过氧化物酶在昆虫卵壳硬化过程中起着关键作用，然而其作为全球性毁灭性害虫（该害虫对常规杀虫剂迅速产生抗性）防治靶标的潜力尚未被探索。本研究对绒毛膜过氧化物酶（）基因进行了功能鉴定，并揭示了其在发育、繁殖及其与肠道微生物群间接关联中的作用。系统发育分析显示，该基因在各物种间具有高度的进化保守性。表达谱表明，该基因在卵巢和卵中高表达，提示其在早期胚胎发育过程中具有强烈的母体贡献。RNA干扰（RNAi）介导的该基因沉默使孵化率降低45%，雌虫产卵力下降42%，并扰乱了幼虫—蛹的转变及成虫羽化。该基因的敲低扰乱了肠道微生物群，降低了多样性，耗竭了 和 等有益菌属，同时富集了 。这些发现表明，该基因可作为可持续害虫防治的双功能靶标。
Abstract
Chorion peroxidase plays a crucial role in the hardening of insect eggshells, yet its potential as a target for pest control in , a globally destructive pest that rapidly develops resistance to conventional insecticides, remains unexplored. In this study, we functionally characterized the chorion peroxidase () gene and its roles in development, reproduction, and its indirect association with gut microbiota. Phylogenetic analysis revealed strong evolutionary conservation of across species. The expression profile showed that was highly expressed in ovaries and eggs, indicating a strong maternal contribution during early embryogenesis. RNA interference (RNAi)-mediated silencing of reduced hatchability by 45%, lowered female fecundity by 42%, and disrupted the larval-pupal transition and adult emergence. Knockdown of perturbed gut microbiota, reducing diversity and depleting beneficial genera such as and , while enriching . These findings identify as a dual-function target for sustainable pest management.
如何引用
AMA
Muhammad Adeel Ghafar, Christopher Rensing, Farman Ullah, Muhammad Asad, Mubashir Tariq, Ritu Raj Poudyal, et al. {"_":"RNAi-Mediated Functional Characterization of Chorion Peroxidase as a Dual-Impact Target for Sustainable Control of .","i":["Spodoptera frugiperda"]}. Journal of agricultural and food chemistry. 2026; doi:10.1021/acs.jafc.6c02764.
APA
Muhammad Adeel Ghafar, Christopher Rensing, Farman Ullah, Muhammad Asad, Mubashir Tariq, Ritu Raj Poudyal, et al (2026). {"_":"RNAi-Mediated Functional Characterization of Chorion Peroxidase as a Dual-Impact Target for Sustainable Control of .","i":["Spodoptera frugiperda"]}. Journal of agricultural and food chemistry. https://doi.org/10.1021/acs.jafc.6c02764
GB/T 7714
Muhammad Adeel Ghafar, Christopher Rensing, Farman Ullah, Muhammad Asad, Mubashir Tariq, Ritu Raj Poudyal, et al. {"_":"RNAi-Mediated Functional Characterization of Chorion Peroxidase as a Dual-Impact Target for Sustainable Control of .","i":["Spodoptera frugiperda"]}[J]. Journal of agricultural and food chemistry, 2026 doi:10.1021/acs.jafc.6c02764.
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