通过底物特异性富集形成的瘤胃衍生联盟显示出专门的木质纤维素利用、多样化的氢代谢和冷冻稳定性
Rumen-Derived Consortia Shaped by Substrate-Specific Enrichment Show Specialized Lignocellulose Utilization, Diversified Hydrogen Metabolism, and Cryopreservation Stability.
摘要(中文译文)
反刍动物瘤胃微生物组对木质纤维素生物质的高效利用对于提高反刍动物饲料效率至关重要，然而稳定且功能特化的微生物群落（microbial consortia）的开发仍然有限。本研究旨在采用生态学引导的富集方法组装底物适应性瘤胃微生物群落。从装有瘤胃瘘管的安格斯×海福特杂交母牛采集瘤胃液，在四种具有不同细胞壁特征的底物上连续富集10代：苜蓿、大麦秸秆、羧甲基纤维素（CMC）和木聚糖。分析了发酵参数（包括产气和挥发性脂肪酸（VFAs））以及细菌群落动态，并对选定的群落（苜蓿和木聚糖）在冷冻保存一个月后的稳定性进行了评估。在整个富集过程中，总VFA浓度下降（例如，木聚糖：109.8 mM（G0）降至56.37 mM（G10）），同时产气减少和α多样性降低，表明存在底物驱动的选择。出现了不同的功能特征，包括苜蓿群落中丙酸增加、大麦秸秆中乙酸较高、CMC中乳酸-丙酸交叉喂养，以及木聚糖富集中与 和 相关的己酸产生（G10时为6.3 mM）。冷冻保存的群落在复苏后保留了核心群落结构和发酵特征。这些结果表明，底物驱动的富集能够产生稳定、功能特化的瘤胃群落，并为开发具有改善瘤胃发酵效率应用潜力的生态兼容微生物群落提供了框架。
Abstract
Efficient utilization of lignocellulosic biomass by the rumen microbiome is critical for improving feed efficiency in ruminants, yet the development of stable, functionally specialized microbial consortia remains limited. This study aimed to assemble substrate-adapted rumen microbial consortia using an ecology-guided enrichment approach. Rumen fluid collected from cannulated Angus × Hereford heifers was sequentially enriched over 10 generations on four substrates with distinct cell wall characteristics: alfalfa, barley straw, carboxymethyl cellulose (CMC), and xylan. Fermentation parameters, including gas production and volatile fatty acids (VFAs), and bacterial community dynamics were analyzed, and selected consortia (alfalfa and xylan) were evaluated for stability following one month of cryopreservation. Across enrichments, total VFA concentrations declined (e.g., xylan: 109.8 mM (G0) to 56.37 mM (G10)), accompanied by reduced gas production and decreased alpha diversity, indicating substrate-driven selection. Distinct functional profiles emerged, including increased propionate in alfalfa consortia, higher acetate in barley straw, lactate-propionate cross-feeding with CMC, and caproate production (6.3 mM at G10) in xylan enrichments associated with and . Cryopreserved consortia retained core community structure and fermentation characteristics upon revival. These results demonstrate that substrate-driven enrichment can generate stable, functionally specialized rumen consortia and provide a framework for developing ecologically compatible microbial communities with potential applications in improving rumen fermentation efficiency.
如何引用
AMA
Ajay Badhan, Chunli Li, Le Luo Guan, Tim A McAllister. Rumen-Derived Consortia Shaped by Substrate-Specific Enrichment Show Specialized Lignocellulose Utilization, Diversified Hydrogen Metabolism, and Cryopreservation Stability.. Microorganisms. 2026; doi:10.3390/microorganisms14051149.
APA
Ajay Badhan, Chunli Li, Le Luo Guan, Tim A McAllister (2026). Rumen-Derived Consortia Shaped by Substrate-Specific Enrichment Show Specialized Lignocellulose Utilization, Diversified Hydrogen Metabolism, and Cryopreservation Stability.. Microorganisms. https://doi.org/10.3390/microorganisms14051149
GB/T 7714
Ajay Badhan, Chunli Li, Le Luo Guan, Tim A McAllister. Rumen-Derived Consortia Shaped by Substrate-Specific Enrichment Show Specialized Lignocellulose Utilization, Diversified Hydrogen Metabolism, and Cryopreservation Stability.[J]. Microorganisms, 2026 doi:10.3390/microorganisms14051149.
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