健康人头皮来源的葡萄球菌KMH304发酵滤液在人毛囊真皮乳头和角质形成细胞中的抗脱发活性
{"_":"Anti-Hair Loss Activity of Healthy Human Scalp-Derived KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells.","i":["Staphylococcus capitis"]}
摘要(中文译文)
头皮微生物被认为是脱发的促进因素，其通过影响头皮稳态和毛发生长发挥作用。然而，源自健康头皮微生物培养物的体外抗脱发活性尚不明确。本研究从20名头皮和毛发健康的韩国受试者中分离出常驻微生物，并利用其生产发酵滤液（SCFF）。在250-1000 μg/mL浓度下，通过增殖实验、qPCR、免疫细胞化学和SA-β-gal染色，在人毛囊真皮乳头细胞（HFDPCs）和人成年低钙高温（HaCaT）角质形成细胞中评估了SCFF的抗脱发活性。SCFF在48小时后以浓度依赖性方式增加细胞密度。在HFDPCs中，SCFF调控生长因子（KGF、IGF-1和HGF）和雄激素相关因子（AR和TGF-β2），调节毛发生长的关键mRNA。SCFF通过促进sirtuins 1和7及胶原蛋白13型，同时抑制p21和X-Gal染色，减轻头皮和毛发衰老。在HaCaT细胞中，SCFF通过显著增加丝聚蛋白和内披蛋白水平，表现出头皮屏障强化作用。它抑制反应性氧化应激，并表现出DPPH和ABTS自由基清除活性。这些结果表明，SCFF可能通过促进头皮和毛发抗衰老、屏障强化、增强抗氧化活性以及支持毛发生长，来调节与脱发相关的关键通路。
Abstract
Scalp microbes are recognized as contributors to hair loss by influencing scalp homeostasis and hair growth. However, the in vitro anti-hair loss activity of microbial culture media derived from healthy scalps remains unclear. In this study, resident microbes from 20 Korean participants with healthy scalps and hair were isolated, and was used to produce ferment filtrate (SCFF). SCFF anti-hair loss activity was evaluated in human follicle dermal papilla cells (HFDPCs) and human adult low-Calcium High-Temperature (HaCaT) keratinocytes via proliferation assays, qPCR, immunocytochemistry, and SA-β-gal staining at 250-1000 μg/mL. SCFF increased cell density after 48 h in a concentration-dependent manner. In HFDPCs, SCFF controlled growth (KGF, IGF-1, and HGF) and androgen (AR and TGF-β2) factors, regulating key mRNAs for hair growth. SCFF mitigated scalp and hair aging by promoting sirtuins 1 and 7 and collagen type 13, while suppressing p21 and X-Gal staining. In HaCaT cells, SCFF exhibited a scalp barrier-strengthening effect by significantly increasing filaggrin and involucrin levels. It suppressed reactive oxidative stress and exhibited DPPH and ABTS radical scavenging activity. These results suggest that SCFF may modulate key pathways associated with hair loss by promoting scalp and hair anti-aging, barrier strengthening, enhancing antioxidant activity, and supporting hair growth.
如何引用
AMA
Hye-Young Yoo, Tae Geun Gil, Na-Rin Kim, Hye-Won Lee, Seoyoung Choi, Sung-Jun Choi, et al. {"_":"Anti-Hair Loss Activity of Healthy Human Scalp-Derived KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells.","i":["Staphylococcus capitis"]}. Microorganisms. 2026; doi:10.3390/microorganisms14040929.
APA
Hye-Young Yoo, Tae Geun Gil, Na-Rin Kim, Hye-Won Lee, Seoyoung Choi, Sung-Jun Choi, et al (2026). {"_":"Anti-Hair Loss Activity of Healthy Human Scalp-Derived KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells.","i":["Staphylococcus capitis"]}. Microorganisms. https://doi.org/10.3390/microorganisms14040929
GB/T 7714
Hye-Young Yoo, Tae Geun Gil, Na-Rin Kim, Hye-Won Lee, Seoyoung Choi, Sung-Jun Choi, et al. {"_":"Anti-Hair Loss Activity of Healthy Human Scalp-Derived KMH304 Ferment Filtrate in Human Hair-Follicle Dermal Papilla and Keratinocyte Cells.","i":["Staphylococcus capitis"]}[J]. Microorganisms, 2026 doi:10.3390/microorganisms14040929.
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