急性弓形虫感染小鼠结肠内容物的脂质组学分析
{"_":"Lipidomic Profiling of Colonic Contents in Mice with Acute Infection.","i":["Toxoplasma gondii"]}
摘要(中文译文)
感染虽构成重大健康威胁，但其对局部肠道脂质代谢的影响仍不明确。因此，本研究建立了BALB/cJ小鼠急性感染模型，并通过非靶向脂质组学分析了感染后10天采集的结肠内容物。两组的脂质谱差异显著，感染组与对照组之间呈现明显分离。我们鉴定出65种上调差异脂质和87种下调差异脂质，主要属于甘油磷脂和鞘脂类。通路富集分析显示，癌症中的胆碱代谢和甘油磷脂代谢通路被确定为感染后扰动最为显著的通路之一。相关性和网络分析进一步提示，LPC (20:4) 和 LPA (18:0) 等关键分子可能通过调节相关酶活性介导这些代谢通路异常。本研究系统刻画了小鼠急性感染期间肠道脂质代谢图谱，揭示了感染诱导的宿主肠道脂质代谢重编程。这些发现为理解感染致病机制及宿主-寄生虫相互作用提供了新的代谢视角。
Abstract
While infection poses a significant health threat, its impact on the localized intestinal lipid metabolism remains unclear. Thus, this study established an acute infection model in BALB/cJ mice and analyzed the colonic contents collected 10 days post-infection via untargeted lipidomics. The lipid profiles of the two groups diverged substantially, with a clear separation evident between infection and control conditions. We identified 65 upregulated and 87 downregulated differential lipids, primarily falling into the glycerophospholipids and sphingolipids categories. Pathway enrichment analysis revealed that the choline metabolism in cancer and glycerophospholipid metabolism pathways was pinpointed as being among the most perturbed following infection. Correlation and network analyses further suggested that key molecules, such as LPC (20:4) and LPA (18:0), may mediate these metabolic pathway abnormalities by regulating related enzymatic activities. This systematic characterization of the intestinal lipid metabolic landscape in mice during acute infection revealed the host intestinal lipid metabolic reprogramming induced by infection. The findings offer a novel metabolic perspective for understanding pathogenesis and host-parasite interactions.
如何引用
AMA
Cai-Qin Deng, Wen-Jie Cheng, Yuan-Lan Wang, Yi-Dan Wang, Feng-Cai Zou, Xing-Quan Zhu, et al. {"_":"Lipidomic Profiling of Colonic Contents in Mice with Acute Infection.","i":["Toxoplasma gondii"]}. Microorganisms. 2026; doi:10.3390/microorganisms14040824.
APA
Cai-Qin Deng, Wen-Jie Cheng, Yuan-Lan Wang, Yi-Dan Wang, Feng-Cai Zou, Xing-Quan Zhu, et al (2026). {"_":"Lipidomic Profiling of Colonic Contents in Mice with Acute Infection.","i":["Toxoplasma gondii"]}. Microorganisms. https://doi.org/10.3390/microorganisms14040824
GB/T 7714
Cai-Qin Deng, Wen-Jie Cheng, Yuan-Lan Wang, Yi-Dan Wang, Feng-Cai Zou, Xing-Quan Zhu, et al. {"_":"Lipidomic Profiling of Colonic Contents in Mice with Acute Infection.","i":["Toxoplasma gondii"]}[J]. Microorganisms, 2026 doi:10.3390/microorganisms14040824.
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