青藏高原水环境中碳青霉烯耐药铜绿假单胞菌的基因组与表型研究
{"_":"Genomic and Phenotypic Insights into Carbapenem-Resistant in the Aquatic Environments of the Tibetan Plateau.","i":["Pseudomonas aeruginosa"]}
摘要(中文译文)
耐碳青霉烯类菌株正日益成为全球健康威胁。然而，尽管水生环境是关键的耐药性储存库，但来自高海拔生态系统的数据仍然匮乏。对从青藏高原水生环境中采集的8株耐碳青霉烯类分离株进行全基因组测序，鉴定出3种序列型（STs），其中ST1420占主导地位（62.5%，5/8）。系统发育分析显示，这些分离株与来自远处临床环境的分离株紧密聚类，提示可能存在跨生境传播。所有研究的菌株均为多重耐药，对亚胺培南、头孢曲松和甲氧苄啶-磺胺甲噁唑的耐药率均为100%。其中包括PA6菌株，该菌株表现出多重抗生素耐药性。8株菌株均携带内在碳青霉烯酶基因。菌株PA2、PA4和PA6的多样化毒力基因谱与其在体外和体内观察到的高致病性相一致。然而，毒力基因型有时与表型并不相关，揭示了仅依赖静态遗传信息的局限性。本研究强调青藏高原水生环境是耐碳青霉烯类菌株的储存库，具有显著的遗传多样性和不同的致病潜力，凸显了其公共卫生相关性。
Abstract
Carbapenem-resistant is increasingly becoming a global health threat. However, although aquatic environments are key resistance reservoirs, data obtained from high-altitude ecosystems are scarce. Whole-genome sequencing of eight carbapenem-resistant isolates collected from aquatic environments in the Tibetan Plateau identified three sequence types (STs), with ST1420 predominating (62.5%, 5/8). Phylogenetic analysis revealed a close clustering of isolates with those from distant clinical settings, suggesting potential cross-habitat transmission. All studied strains were multidrug-resistant, exhibiting 100% resistance to imipenem, ceftriaxone, and trimethoprim-sulfamethoxazole. This included the PA6 strain, which showed multiple-antibiotic resistance. Eight strains harbored the intrinsic carbapenemase gene . The diverse virulence-gene profiles of strains PA2, PA4, and PA6 aligned with their high pathogenicity observed both in vitro and in vivo. However, virulence genotypes sometimes did not correlate with phenotypes, revealing the limitations of relying on static genetic information alone. This study highlights the aquatic environments of the Tibetan Plateau as reservoirs of carbapenem-resistant with substantial genetic diversity and divergent pathogenic potential, underscoring their public-health relevance.
如何引用
AMA
Dingxiang Lu, Lin Liu, Zhongwei Yang, Tianjiao Chen, Dong Yang, Danyang Shi, et al. {"_":"Genomic and Phenotypic Insights into Carbapenem-Resistant in the Aquatic Environments of the Tibetan Plateau.","i":["Pseudomonas aeruginosa"]}. Microorganisms. 2026; doi:10.3390/microorganisms14051094.
APA
Dingxiang Lu, Lin Liu, Zhongwei Yang, Tianjiao Chen, Dong Yang, Danyang Shi, et al (2026). {"_":"Genomic and Phenotypic Insights into Carbapenem-Resistant in the Aquatic Environments of the Tibetan Plateau.","i":["Pseudomonas aeruginosa"]}. Microorganisms. https://doi.org/10.3390/microorganisms14051094
GB/T 7714
Dingxiang Lu, Lin Liu, Zhongwei Yang, Tianjiao Chen, Dong Yang, Danyang Shi, et al. {"_":"Genomic and Phenotypic Insights into Carbapenem-Resistant in the Aquatic Environments of the Tibetan Plateau.","i":["Pseudomonas aeruginosa"]}[J]. Microorganisms, 2026 doi:10.3390/microorganisms14051094.
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