MicrobiologyOpen微生物学开放
MicrobiologyOpen(英文缩写 MICROBIOLOGYOPEN),ISSN 2045-8827,eISSN 2045-8827,中文译名:微生物学开放 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.904 | Q3 |
| 2022 | 3.400 | Q2 |
| 2023 | 3.900 | Q2 |
| 2024 | 4.600 | Q1 |
| 2025 | 3.600 | Q2 |
MicrobiologyOpen 最新收录文献
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1. Evaluation of Probiotic Properties and Antimicrobial Substances Produced by Five Lactic Acid Bacteria Against Foodborne and Spoilage Pathogens.
PMID:日期:2026-10-01The rise of multidrug-resistant (MDR) pathogens presents a major challenge to food safety, particularly in preservation approaches. Lactic acid bacteria (LAB) gain probiotic recognition by effectively controlling MDR pathogens using antimicrobial substances (AMSs) and bacteriocin-like inhibitory substances (BLISs). The study evaluated the probiotic properties and AMS production potential of five LAB strains: Lactiplantibacillus plantarum NBRC 3070 (LP), Lactobacillus acidophilus ATCC 4356 (LA), Lacticaseibacillus casei ATCC 393 (LC), Lacticaseibacillus rhamnosus GG ATCC 53103 (LGG), and Bifidobacterium animalis subsp. lactis ATCC 27673 (BAL). AMS was extracted from neutralized and concentrated cell-free supernatant and tested for antimicrobial activities against common foodborne pathogens using minimum inhibitory concentrations (MICs). All LAB strains showed antagonistic activity, with LP demonstrating the most substantial antagonism. Four strains are resistant to multiple antibiotics but remain susceptible to azithromycin and tetracycline, while LP is resistant to all tested antibiotics. LP and LC demonstrated the highest probiotic potential based on cell surface hydrophobicity and aggregation abilities. Optimal AMS activity (85.36%-91.29%) occurred between 24 and 36 h at 37°C or 28-48 h at 30°C. LGG exhibited the highest AMS activity with the lowest MIC against Salmonella enterica serovar Typhimurium. Enzyme sensitivity confirmed the peptide nature of BLIS produced by BLIS from LP, LA, LC, and LGG. In conclusion, LAB strains demonstrated significant probiotic traits and the ability to produce AMS, especially BLIS, from four strains, with LP and LC showing the most potential. These findings support their application as natural biopreservation against MDR pathogens; however, further validation in the food system is required.
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2. Carotenoid-Rich Pigment Extract From Micrococcus terreus SK34 Induces Apoptosis in SH-SY5Y Neuroblastoma Cells: Integrated Metabolomic, Molecular Docking, and Experimental Validation.
PMID:日期:2026-10-01Bacteria-derived carotenoids have attracted increasing attention as natural bioactive compounds with potential pharmaceutical applications, particularly in cancer therapy. However, the chemical composition and anticancer mechanisms of carotenoids from Micrococcus terreus remain largely unexplored. This study aimed to characterize the carotenoid extract from the locally isolated M. terreus SK34 strain (GenBank: PX765938) and to investigate its anticancer activity and underlying mechanisms using integrated in vitro and in silico approaches. Under nonoptimized culture conditions, strain SK34 produced 128 mg/L of carotenoid-rich pigment extract. LC-Q-TOF-MS analysis identified eight carotenoids, including phytoene, β-carotene, astaxanthin, zeaxanthin, β-cryptoxanthin, α-carotene, lycopene, and sarcinaxanthin. The extract exerted selective cytotoxicity against different cancer cell lines (IC = 61.91-243.76 μg/mL), particularly the neuroblastoma (SH-SY5Y) cancer cell line, while showing minimal cytotoxicity toward normal human dermal fibroblasts. Gene expression analysis demonstrated the significant upregulation of proapoptotic genes (p53, BAX, CASP3, CASP8, and CASP9) in the SH-SY5Y cell line, together with the downregulation of antiapoptotic genes (BCL-2 and AKT1), whereas autophagy-related genes were not significantly affected. ELISA and flow cytometry analyses further confirmed apoptosis in the SH-SY5Y cell line. Molecular docking analyses revealed that β-carotene, astaxanthin, and zeaxanthin exhibited strong binding affinities toward apoptosis-related proteins, supporting the experimental findings. This study provides the first comprehensive characterization of carotenoids from M. terreus SK34 and highlights their potential as selective apoptosis-inducing agents for future anticancer therapy.
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3. An Integrated System for Geobacillus Protoplast Formation, Transfection, Recombinant Genomes Rebooting, Efficient Propagation, and Genomic DNA Isolation of the Thermophilic Bacteriophage TP-84.
PMID:日期:2026-10-01Protoplast transformation has been an effective method for introducing plasmid DNA into Bacillus species; however, its application to deliver complete bacteriophage genomes has not been demonstrated previously. Here, we describe an optimized and reproducible method for forming Geobacillus stearothermophilus protoplasts and efficiently transfecting them with purified recombinant or wild-type genomic DNA from the thermophilic bacteriophage TP-84. Systematic optimization of key parameters, including bacterial cell density, lysozyme concentration, polyethylene glycol type and concentration, and DNA input, enabled robust phage genomic DNA uptake and subsequent production of infectious phage particles. In addition, we optimized and scaled up a single-layer solid-state bacteriophage propagation procedure for integration into the TP-84 genome-engineering workflow, enabling the recovery of high-titer lysates and sufficient genomic DNA for sequencing and subsequent genome manipulation from a single Petri dish. In our experimental system, solid-state propagation also provided greater stability of recombinant phage variants than propagation in liquid bacterial cultures. Together, these methods establish an integrated protoplast-based system for phage genomic DNA delivery, rebooting, propagation, and recovery, enabling controlled manipulation of bacteriophage genomes and facilitating the rapid generation and analysis of recombinant variants.
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4. Single-Step Production of Mannitol From Yacon Fructo-Oligosaccharides Using Fructophilic Lactic Acid Bacteria.
PMID:日期:2026-10-01Mannitol is a naturally occurring sugar alcohol with several applications in the food and pharmaceutical industries, with special interest as a low-calorie sweetener. While mannitol can be synthesized chemically, synthesis by bacterial fermentation is an attractive means of production due to its low cost, mild (near-ambient) conditions for synthesis and high yield. Bacterial production also allows the possibility of using low-cost, second-generation feedstocks to produce mannitol in a sustainable manner. In this work, we describe the feasibility of producing mannitol from yacon (Smallanthus sonchifolius) tuber using Leuconostoc mesenteroides and Fructobacillus fructophilus previously isolated from floral sources. The tuber contains over 50% fructooligosaccharides (FOS) which was found to be bioavailable for mannitol production without prior hydrolysis or saccharifying pretreatments. Initial screening of several fructose-tolerant lactic acid bacteria (LAB) led to the selection of the most robust mannitol-producing strains for optimizing culture conditions and scale-up. We ultimately demonstrated the successful production of up to 7 g/L mannitol using L. mesenteroides with 2% yacon root powder in 200 mL bioreactors, achieving a yield greater than 0.6 g mannitol/g FOS with no significant decrease in titers as the scale of culture is increased. Our study demonstrates that yacon powder is a suitable and cheap substrate for the sustainable and economical production of an important sugar alcohol by biotransformation. Finally, we discuss future approaches to increase the scale and purity of production.
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5. Exploring Cervical Cancer-Associated Vaginal and Cervical Microbiota via 16S rRNA Sequencing.
PMID:日期:2026-10-01Although the etiology of cervical cancer has been partially established, its pathogenesis remains a key research focus. Specific studies on the genital tract microbiota of cervical cancer patients in Southern China are still scarce. To address this research gap, the present study collected genital tract microbial samples from 16 cervical cancer patients and 16 healthy women. Analysis of cervical cancer-specific microbiota was conducted via 16S rRNA gene sequencing. The study included 32 women (16 cervical cancer patients and 16 healthy controls) aged 45-65 at enrollment. Total genome DNA from samples was extracted using the hexadecyltrimethylammonium bromide (CTAB) CTAB method. The vaginal and cervical microbiota composition was determined by sequencing barcoded 16S rDNA gene fragments (V3-V4), and a comparative bioinformatics analysis of the microbiome was performed. The study revealed a homogeneous microbial composition dominated by Lactobacillus in healthy women, whereas cervical cancer patients exhibited increased diversity with reduced Lactobacillus and enriched Prevotella. No significant differences were observed between sampling sites within each group. Functional predictions linked the cancer-associated microbiota to metabolic pathways and the AEROBACTIN-SYN-PWY pathway. In conclusion, our findings suggest that these specific key microbial taxa and their related metabolic pathways contribute to the pathogenesis of cervical cancer and may serve as promising targets for clinical treatment and intervention.
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6. Improving Agar Plate Image Quality Using a Low-Cost 3D-Printed Lightbox: A Proof-of-Concept Evaluation for Machine Learning-Assisted Analysis.
PMID:日期:2026-10-01Agar plate photography is widely used to document microbial growth, but images captured under routine laboratory lighting are often affected by glare, reflections, shadows, and uneven illumination, which may compromise both visual interpretation and computational analysis. This proof-of-concept study developed and evaluated a low-cost, three-dimensional (3D)-printed lightbox for standardized smartphone-based agar plate imaging. Image characteristics were assessed across five uncultured agar media under traditional and lightbox conditions using Fiji/ImageJ. Machine learning-assisted analysis was then performed on cultured horse blood agar plates using QuPath. Separate Random Trees pixel classifiers were developed for traditional and lightbox images to distinguish colonies from background agar and noncolony artifacts. A semiautomated QuPath-assisted workflow was also compared with manual colony counts across 14 growth-positive horse blood agar plates. Lightbox imaging reduced reflective artifact, improved illumination consistency, and enhanced visualization of colony margins and hemolysis-associated changes. Traditional-lighting images produced more artifact-related classifier detections, including glare, reflections, and uncolonized agar being detected as colony-like regions. In the exploratory colony-count comparison, semiautomated QuPath-assisted counts showed close agreement with manual reference counts (Pearson r = 0.999; mean absolute error = 2.4 colonies per plate). These findings demonstrate that a low-cost, 3D-printed lightbox can improve the quality and standardization of smartphone-based agar plate images and support more interpretable machine learning-assisted analysis. Further validation using larger independent data sets, additional organisms, media types, and imaging devices is required.
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7. Interrogating the Escherichia coli Epitranscriptome Via CRISPR Interference and Nanopore Native RNA Sequencing.
PMID:日期:2026-10-01Epitranscriptomics has recently gained significant momentum due to technological advances and translational applications; however, studies on bacterial RNA modifications remain limited. Bacterial RNA is notoriously prone to degradation, and methodologies to investigate the epitranscriptome are challenging. Prior research has shown RNA modifications modulate antimicrobial resistance, virulence and pathogenicity. This research employed CRISPR interference to knock down five known Escherichia coli rRNA modification genes (rlmF, rlmJ, rluD, rsmF and rsmG) in three E. coli strains. These isolates were investigated for growth delays, changes in the proteome and the influence on mRNA modifications via native RNA sequencing. CRISPRi adequately silenced the majority of RNA modification genes in E. coli (> 80% reduction). Significant growth delays were associated with rlmF, rluD and rsmF repression. Unique protein pathways corresponding with RNA modification loss were found for rlmJ (TreB, XylF), rluD (CysH, HycB, PutP, TrpB), rsmF (EvgA) and rsmG (OppC). Known rRNA modification sites for rluD (Ψ) and rsmG (m7G) were detected from analysis of nanopore electrical signal; however, only a weak signal was apparent for m6A (rlmF, rlmJ) and m5C (rsmF) modifications. The inhibition of rRNA modifications resulted in mRNA modification changes, including for genes ompC, cspC, dbhA, dbhB and secY. Our work provides an approach for unravelling the epitranscriptome of E. coli to gain insight into its functional role.
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8. Proteomic Analysis of Three Independent Series of Sequential Cystic Fibrosis Strains in an International Pseudomonas aeruginosa Reference Panel Indicates Positive Selection in Late Infection Strains.
PMID:日期:2026-10-01Pseudomonas aeruginosa is a highly diverse, adaptable Gram-negative bacterium that thrives in many environments and is a frequent cause of chronic opportunistic infections in people with cystic fibrosis (CF). P. aeruginosa adapts over time of colonization to facilitate chronic infection, including loss of virulence factors; however, proteomic analyses of the adaptation to chronic infection have been limited. We previously assembled and characterized an international panel of P. aeruginosa strains from diverse clinical and geographical sources, including three sets of sequential CF isolates, enabling identification of conserved adaptations linked to chronic CF lung colonization. We compared the proteomes of eight strains (three early and five late infection) to assess whether common proteomic changes emerged during colonization across the sequential isolates. We identified 11 proteins showing increased abundance in late isolates in all three series, many of which are associated with virulence, regulation of virulence, or response to hypoxia. These include CF inhibitory factor repressor (CifR); WspR; 2 two-component response regulators (PA2572 and PA3702), and transcriptional regulator (PA2551). Moreover, we identified three proteins (PA2572, PA3819, and PA5028) that showed increased abundance in all five late isolates. The probability of this being random is 5.06 × 10 and therefore, strong evidence of positive selection. The increased abundance of PA2573 and PA3819 appears to improve the fitness of P. aeruginosa in response to antibiotics and oxidative stress. All three proteins share a tyrosine phosphorylation motif, suggesting a common regulatory mechanism. Overall, despite substantial diversity across P. aeruginosa, common adaptations occur in the CF lung.
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9. A Post-NGP Mitsuokella jalaludinii as a Therapeutic Candidate for Gout.
PMID:日期:2026-10-01Gout is a common inflammatory disease closely associated with hyperuricemia, and its global prevalence is increasing, highlighting the need for safer and more effective therapeutic strategies. Recently, microbiome-based therapeutics have emerged as promising alternatives, and the development of functionally validated NGP and their advanced forms, post-NGP strains, is gaining increasing attention. In this study, microbiome-derived bacterial strains were screened based on their in vitro uric acid (UA)-reducing capacity and hypoxanthine utilization. Among the tested strains, Mitsuokella jalaludinii exhibited the most pronounced uric acid reduction and growth enhancement in response to purine substrates compared to conventional lactic acid bacteria. This strain reduced uric acid levels by approximately 30% after 48 h (p < 0.001) and showed significantly increased growth in purine-enriched minimal medium (p < 0.01). WGS further confirmed its strain-level novelty, leading to its designation as PMC73, and revealed the presence of genetic features associated with purine metabolism and UA regulation. In a MSU-induced RAW 264.7 macrophage model, PMC73 significantly reduced UA levels (p < 0.001) and modulated immune responses by increasing anti-inflammatory cytokines while decreasing pro-inflammatory cytokines. These effects were associated with suppression of the NLRP3-caspase-1, along with downregulation of URAT1 and XOD, indicating coordinated regulation of uric acid production, reabsorption, and inflammatory pathways. Based on safety evaluation, including d-lactate production, cytotoxicity assays and oral toxicity assessment in a mouse model, demonstrated that PMC73 exhibited no pathogenic or toxic properties. Therefore, PMC73 represents a promising microbiome-based candidate with relevance to gout-associated hyperuricemia, warranting further in vivo and clinical investigations.
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10. Insights Into the Antibiofilm Activity of Carbon Quantum Dots Against a Panel of Different Microorganisms: A Review.
10. 碳量子点对不同微生物抗菌膜活性的见解：综述PMID:日期:2026-10-01Biofilm-associated infections account for a majority of chronic bacterial diseases. These infections also present a therapeutic challenge due to their intrinsic tolerance to conventional antibiotics. Carbon dots (CDs) have emerged as a versatile nanoplatform with the potential to combat these resilient structures. This review critically evaluates the burgeoning field of CD-based antibiofilm agents. We move beyond a catalog of studies to analyze how the physicochemical properties of CDs dictate their mechanisms of action against a panel of microorganisms. The antibiofilm activity of CDs is multi-sided, stemming from their ability to (1) electrostatically interact with and disrupt the biofilm matrix and bacterial cell envelopes, (2) penetrate deep into biofilms due to their ultrasmall size, (3) generate ROS that degrade the extracellular polymeric substance (EPS) and kill embedded cells, and (4) downregulate key genes involved in quorum sensing and biofilm formation. An analysis of the literature reveals an efficacy bias towards Gram-positive bacteria, highlighting the barrier posed by the outer membrane of Gram-negative pathogens. We dissect how synthetic strategies and surface passivation techniques (e.g., with cationic polymers, lysozyme, or targeting ligands) are being deployed to overcome these challenges. While CDs represent a frontier in antibiofilm therapy, the field faces hurdles, including a lack of standardized testing protocols, limited in vivo validation, and unresolved questions about toxicity. This review identifies these critical gaps and proposes future research directions focused on the rational design of next-generation CDs with enhanced specificity, potency, and translational potential for treating recalcitrant biofilm infections.