VIROLOGY病毒学
VIROLOGY(英文缩写 VIROLOGY),ISSN 0042-6822,eISSN 1089-862X,中文译名:病毒学 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-09-27 至 2026-09-27，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 3.513 | Q3 |
| 2022 | 3.700 | Q3 |
| 2023 | 2.800 | Q3 |
| 2024 | 2.400 | Q3 |
| 2025 | 2.600 | Q3 |
VIROLOGY 最新收录文献
-
1. Immunopathogenesis of TB-HIV Co-Infection: Mechanisms of Dual Immune Suppression and Viral-Bacterial Synergy.
PMID:日期:2026-12-01Human immunodeficiency virus (HIV) and Mycobacterium tuberculosis (M. tuberculosis) co-infection represents one of the most devastating syndemics in global health, accounting for substantial morbidity and mortality worldwide. The interaction between these two pathogens is not merely additive but highly synergistic, driven by profound immune dysregulation. HIV-mediated depletion of CD4 T cells facilitates tuberculosis (TB) progression, whereas TB-induced immune activation enhances HIV replication and accelerates disease progression. HIV preferentially infects CD4 T cells and impairs the function of macrophages and dendritic cells, resulting in defective granuloma formation, impaired antigen presentation, and chronic systemic inflammation. Conversely, active TB promotes sustained production of cytokines and chemokines, creating a permissive environment for increased HIV transcription, viral dissemination, and maintenance of viral reservoirs. This review synthesizes current knowledge on the immunopathogenesis of TB-HIV co-infection, with particular emphasis on the mechanisms underlying dual immune suppression, including granuloma destabilization, macrophage dysfunction, impaired antigen presentation, immune exhaustion, cytokine imbalance, and disruption of mucosal and tissue immune homeostasis. We further examine how the bidirectional interplay between HIV and M. tuberculosis reshapes both innate and adaptive immune responses, contributing to atypical clinical manifestations, diagnostic challenges, immune reconstitution inflammatory syndrome (IRIS), and poor treatment outcomes. Finally, we discuss emerging host-directed and immunomodulatory therapeutic strategies, together with the growing role of systems biology and computational approaches in identifying immune correlates and predictive biomarkers. A deeper understanding of these interconnected mechanisms will be essential for developing integrated, personalized therapeutic strategies to improve clinical outcomes in individuals with TB-HIV co-infection.
-
2. A conserved distal-tail helical extension defines a tailspike attachment architecture in Gram-negative siphophages.
PMID:日期:2026-12-01Rapid growth of bacteriophage genome collections has outpaced functional annotation of tail-tip proteins, limiting comparative analysis of host-recognition structures. Starting from a shared distal-tail gene organization in the Salmonella phages 9NA and Jersey, I developed a morphogenetic bioinformatic framework integrating gene synteny, sequence comparison, profile hidden Markov model (HMM) screening, structural evidence, structure-aware searching, and AlphaFold modeling. Comparison with the experimentally characterized lambda and Sf11 tail assemblies identified a predominantly alpha-helical C-terminal extension of the distal-tail (DT) protein associated with tailspike attachment, termed the distal-tail helical extension (DT-helix). Screening 541,986 proteins from 5167 complete NCBI RefSeq tailed-phage genomes, followed by evidence-based evaluation of sequence, genomic context, and structural architecture, identified 165 curated DT-helical-extension-associated phages. Their DT proteins segregated into six sequence groups. In the four principal multi-member groups, cognate tailspikes showed group-specific conservation in proximal N-terminal regions but substantially greater downstream diversity, consistent with sequence constraint at the DT-tailspike attachment boundary. A complementary ProstT5/Foldseek search supported the established groups but revealed no convincing additional highly divergent family. Together with the experimentally characterized Sf11 attachment interface, these findings define a recurrent morphogenetic architecture linking conserved distal-tail scaffolds to more variable receptor-binding proteins across siphophages infecting Gram-negative bacteria. Although universal exchangeability is not established, the identified scaffold-receptor-binding boundaries provide a framework for molecular characterization and rational phage engineering. Accession-level information for the 165 curated phages is available through PhageTailDB.
-
3. SUMO1 modification stabilizes polymerase protein VP1 of Blotched Snakehead Virus.
PMID:日期:2026-12-01SUMOylation, the covalent attachment of SUMO molecules to target proteins, is a post-translational modification (PTM) that plays essential roles in the replication of many viruses. In this study, we demonstrate that SUMO1 effectively conjugates to VP1, the RNA-dependent RNA polymerase (RdRp) of Blotched Snakehead Virus (BSNV). SUMO1 modification of BSNV VP1 requires the active sites of both SUMO1 and Ubc9. Site-directed mutagenesis revealed that the SIM motif (IYIY) of VP1 is essential for its SUMOylation. Notably, the SIM mutant VP1 exhibited significantly reduced protein stability compared to wild-type (WT) VP1, and this degradation was markedly suppressed by the proteasome inhibitor MG132. Mechanistically, the SIM mutant VP1 showed increased levels of both total and K48-linked ubiquitination, explaining its instability. Our findings reveal that SUMOylation of BSNV VP1 stabilizes the polymerase by antagonizing ubiquitin-proteasome-mediated degradation, providing novel insights into the regulation of birnavirus replication.
-
4. Site-specific microRNA responses in lungs of pigs depend on the host-adaptation of H1N1 influenza A virus.
PMID:日期:2026-12-01Cross-species transmission of influenza A virus (IAV) represents a major risk for emergence of new pandemics, however, the host molecular responses invoked by cross-species IAV infection remain incompletely defined. MicroRNAs (miRNAs) are important post-transcriptional regulators of innate immunity, but their role during infection with IAV strains of the same subtype but of different host origin has not been investigated. Here, we investigated pulmonary miRNA responses in upper and lower lung sites from pigs inoculated with either a swine-adapted (swH1N1) or a human-adapted (huH1N1) IAV originating from the 2009H1N1 pandemic strain. High-throughput microfluidic RT-qPCR profiling of 115 miRNAs and selected antiviral genes revealed strain-dependent differences, with 33 miRNAs being differentially expressed in pig lungs (upper and lower lung) after human-adapted IAV infection, while only eight miRNAs were altered after swine-adapted IAV infection. Expression of several miRNAs correlated inversely with predicted target pattern recognition receptor (PRR) and interferon-stimulated gene (ISG) transcripts after huH1N1 infection, including miR-19a/b, miR-21-5p, and miR-93-3p, suggesting miRNA-mediated fine-tuning of antiviral signaling. Notably, extensive downregulation of miRNAs in the lower lungs after huH1N1 infection coincided with robust ISG induction, despite huH1N1 viral lung load being comparable to swH1N1 viral load. The swine-adapted IAV, in contrast, triggered stronger RIG-I-like receptor and cGAS transcription without substantial miRNA changes. Together, these findings demonstrate that pulmonary miRNA responses to IAV are both strain- and lung site-specific and may contribute to shaping the outcome of infection depending on the degree of viral host adaptation.
-
5. Metatranscriptomic analysis of viral sequences associated with Culex nigripalpus at an Alabama aquaculture site.
PMID:日期:2026-12-01Mosquitoes associated with aquaculture habitats can harbor diverse viruses, yet the viromes of many locally abundant species remain poorly characterized. At an aquaculture-associated site in Auburn, Alabama, we surveyed mosquito populations and found Culex nigripalpus to be the dominant species collected. To characterize viruses associated with this mosquito, we performed RNA-seq on pooled female Cx. nigripalpus and compared complementary bioinformatic workflows for viral detection and genome recovery. One workflow removed host-associated reads by mapping to the closest available mosquito reference genome prior to assembly, whereas a second workflow used fully de novo assembly and viral database annotation. Additional protein-level filtering, cross-workflow comparison, and comparison of Trinity and rnaSPAdes assemblies were used to prioritize well-supported viral candidates. Across the original analyses, 16 submitted accessions corresponding to 12 collapsed virus/name groups were recovered, including Merida virus, Hubei mosquito virus 5, Zhejiang mosquito virus, Hubei virga-like virus 3, Rinkaby virus, Elemess virus, Qingnian mosquito virus, Serbia narna-like virus 2, XiangYun narna-levi-like virus 8, Ecclesville picorna-like virus, and baculovirus-like fragments. Several candidates were supported across multiple workflows, while others were recovered only under specific analytical conditions, indicating that candidate recovery was influenced by assembly and filtering choices. Selected viral contigs were independently supported by RT-PCR amplification. Overall, these results provide a first characterization of viral sequences associated with Cx. nigripalpus from an Alabama aquaculture-associated site and show that comparison across assembly and filtering strategies helped prioritize the most consistently supported viral candidates.
-
6. AI-enabled viral genomics: from virus discovery to host prediction and emerging variant forecasting.
PMID:日期:2026-12-01The rapid expansion of metagenomic sequencing has generated vast repositories of viral sequence data that far outpace our capacity to interpret them using conventional approaches. Highly divergent sequences, sparse functional annotation, and taxonomically uneven sampling present fundamental challenges for reference-dependent methods, which lose sensitivity precisely for novel and understudied viruses with high public health relevance. Artificial intelligence (AI) provides a new avenue to address these challenges by enabling predictive inference from viral genomes and proteins while reducing dependence on sequence similarity. In this Review, we discuss representative advances in AI for virus discovery, taxonomic classification and functional annotation, prediction of host range and zoonotic potential, and efforts toward forecasting emerging variants. These advances are transforming viral genomics from a largely descriptive discipline into one with increasing predictive capability. We also critically assess the major challenges that constrain current approaches, including the availability of high-quality and representative datasets, rigorous model evaluation, biological interpretability and responsible governance for increasingly capable AI models.
-
7. The immunoproteasome is protective during acute murine hepatitis virus respiratory infection but dispensable for adaptive immunity.
PMID:日期:2026-12-01The immunoproteasome (IP) is a component of cellular proteasome machinery that is predominantly inducible by IFN-γ. IP activity contributes to multiple aspects of immune function, inflammation, and protein homeostasis. IP subunit expression increases during respiratory infection with murine hepatitis virus strain 1 (MHV-1), a mouse coronavirus, and pharmacologic inhibition increased virus-induced inflammation and mortality. In this study, we used mice that are completely deficient in IP activity to evaluate effects of IP deficiency, instead of IP inhibition, on MHV-1 pathogenesis. IP deficiency had no effect on viral replication in lungs or livers in mice infected with a low dose, but viral replication was greater in livers of mice infected with a high dose. IP deficiency altered expression of IFN-γ and other cytokines in an organ- and virus dose-dependent manner. MHV-1 infection increased expression of genes associated with endoplasmic reticulum stress response, but there were minimal differences between IP-deficient and wild type mice. IP deficiency tended to decrease the amount of total virus-specific IgG. However, neutralizing antibody and protective immunity to repeat MHV-1 infection was intact in IP-deficient mice. Our results indicate that the IP is a protective host factor during acute MHV-1 infection, confirming our previous findings with IP inhibition. However, the results suggest that the IP is dispensable for the development of adaptive immune responses and protective immunity to MHV-1.
-
8. Fluid shear stress delays viral gene expression in a human vascular model.
PMID:日期:2026-12-01Viral dissemination through blood vessels is a part of systemic infection and causes endothelial cells to be exposed to viruses. Inside human vasculature, hemodynamic forces exert fluid shear stress (FSS) against blood vessel walls; however, its impact on viral infection remains understudied. To explore the impact of FSS on viral infections, we characterized Vesicular Stomatitis Virus (VSV) and replication-competent, recombinant VSV (rVSV) infections under static and FSS conditions. Infections in either VeroE6 or primary endothelial cells were performed within a microphysiological system (MPS), capable of variable flow rates. We found that when the MPS was seeded with VeroE6 cells both WT VSV and rVSVs were capable of robust infection under static and FSS conditions. In contrast, FSS delayed WT VSV infection and viral spread in primary endothelial cells. Infection with rVSVs increased delays in infection in both cell types, and rVSVs containing an additional gene insertion demonstrated the most attenuation under FSS. These results suggest that FSS can have an important impact on virus infection that is independent of the entry protein used and dependent on genetic modification.
-
10. Getah virus, a re-emerging arbovirus: epidemiology, pathogenesis, diagnostics and biosecurity.
PMID:日期:2026-12-01The re-emergence of neglected vector-borne viruses has attracted attention globally in recent years. Getah virus (GETV), a mosquito-borne alphavirus first discovered in 1955 in Malaysia, caused sporadic mild clinical disease in horses in Asia. A highly pathogenic strain of GETV emerged in 2014 in Japan and spread to China causing multiple outbreaks in horses, pigs, and blue foxes with an expanding geographical distribution. Notably, there appears to be continued active transmission of GETV between China and Japan. While infections in horses are often mild and self-limiting, infected blue foxes, pregnant sows and piglets can present with reproductive and neurological signs. Mortality in piglets can reach up to 100% whereas adult pigs suffer from high morbidity without mortality. A case fatality rate of 24% was reported in blue foxes. This review provides an update on current understanding of the epidemiology and pathogenesis of GETV infection and addresses the diagnostic and biosecurity considerations for disease investigation, diagnosis, and management.