MICROCHEMICAL JOURNAL微化学杂志
MICROCHEMICAL JOURNAL(英文缩写 MICROCHEM J),ISSN 0026-265X,eISSN 1095-9149,中文译名:微化学杂志 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 5.304 | Q1 |
| 2022 | 4.800 | Q1 |
| 2023 | 4.900 | Q1 |
| 2024 | 5.100 | Q1 |
| 2025 | 5.200 | Q1 |
MICROCHEMICAL JOURNAL 最新收录文献
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1. Development and Validation of an Analytical Method for Simultaneous Quantitation of Individual Xylene Isomers in Blood via Headspace Gas Chromatography-Mass Spectrometry.
1. 通过顶空气相色谱-质谱联用法同时定量血液中个体二甲苯异构体的分析方法的开发与验证PMID:期刊:日期:2025-03-21Xylenes are ubiquitous in the environment due to their widespread use in a variety of products and industries, including cleaning agents, paint thinners, printing, and plastic production, demonstrating the potential for exposure in humans. Because of limited data on xylenes, we are investigating the toxicity of xylenes, mixed in a specific isomeric ratio, in rodent models. The goal of this study was to develop and validate a method to quantitate individual xylene isomers (p-, m-, and o-xylene) in rodent blood in support of toxicity studies. The method used internal standard calibration with isotopically labeled analytes for an analysis by headspace gas chromatography-mass spectrometry following removal of moisture with anhydrous calcium chloride to allow separation of individual isomers. In male Sprague Dawley rat blood, the method was linear ( ≥ 0.99) over the range of 5 - 1500 ng/mL, accurate (mean relative error (RE) ≤ 15.3%), and precise (relative standard deviation (RSD) ≤ 10.8%). Low limits of detection were achieved, at 1.0, 2.3, and 1.0 ng/mL for p-, m-, and o-xylene, respectively. The method was evaluated in male and female Hsd:Sprague Dawley SD rat blood and male and female B6C3F1/N mouse blood, the strains used in toxicology studies, with RE ≤ ±13.5% and RSD ≤ 10.6%. All isomers were stable for at least 62 days in blood when stored frozen. The results presented here demonstrate this method can accurately and precisely quantitate p-, m-, and o-xylene in a single analytical run in rat and mice blood samples following exposure to individual xylene isomers. The method could be easily adapted to other biological matrices, including human matrices.
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2. Bioanalytical considerations for quantification of ondansetron in rat microdialysis study using LC-MS/MS.
PMID:期刊:日期:2025-02-18Ondansetron is an anti-emetic drug and has been recently identified as a therapeutic for neuropathic pain. Permeability of ondansetron into the nervous system may be impacted by the presence of P-glycoprotein (Pgp) at the blood brain barrier, since ondansetron is a substrate of Pgp, which means it may not easily cross into the brain. In order to determine a drug concentration within the brain, microdialysis can be used, which measures unbound analyte concentrations in tissues or body fluids. The purpose of this study was to develop and validate an LC-MS/MS method to measure ondansetron concentrations in serum and brain microdialysates for pharmacokinetic studies in rats. To the best of our knowledge, a fully validated method for the quantification of ondansetron in brain microdialysis studies has not been reported so far. Chromatographic separation was achieved at a flow rate of 0.6 mL/min using the Gemini C18 column (5 μm; 50 × 4.6 mm) and a mobile phase consisting of 10 mM ammonium formate with 0.1 % formic acid in water and 0.1 % formic acid in acetonitrile (55:45, v/v). Serum samples were prepared by protein precipitation; for microdialysate, no specific cleaning procedure was needed, they were only diluted with the internal standard solution. The presented method requires only a small volume of serum (2.5 μL) and microdialysate (15-20 μL), which allows for frequent sampling in animals. Moreover, it enables high throughput due to a short run time and straightforward sample preparation. Artificial cerebrospinal fluid (aCSF) was confirmed to be useful as a surrogate matrix for brain microdialysate. The lower limit of quantitation for ondansetron was 0.01 μg/mL for serum and 0.025 ng/mL for microdialysate. A significant ion suppression for ondansetron was observed in brain microdialysate and aCSF, but the IS-normalized matrix factor was close to 1.0. The developed LC-MS/MS method met the FDA and EMA validation criteria and was successfully applied to the in vivo pilot pharmacokinetic study. It was sensitive enough to capture the low ondansetron concentrations in brain microdialysate and universal enough to measure high ondansetron concentrations in rat serum.
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3. Development and application of a non-targeted analysis method using GC-MS and LC-MS for identifying chemical contaminants in drinking water via point-of-use filters.
PMID:期刊:日期:2024-12While many chemicals are regulated and routinely monitored in drinking water, they represent just a portion of all contaminants that may be present. Typical drinking water analyses involve sampling one liter or less of water, which could lead to trace level contaminants going undetected. In this study, a method was developed for using point-of-use activated carbon block drinking water filters as sampling devices. The filters were extracted to remove chemicals that were collected, and then analyzed by non-targeted analysis via liquid chromatography and gas chromatography high-resolution mass spectrometry. Extraction efficiencies were assessed by spiking and recovery experiments to better understand the chemical space coverage. To test the method's applicability to real-world samples, filters from a small-scale pilot study were collected from individuals in New York, NY and Atlanta, GA and analyzed. Twenty tentatively identified chemical candidates were confirmed by comparison to chemical standards. Principal components analysis was performed on the full set of filtered chemical features to explore how geographic and temporal differences in samples impact drinking water composition. Product use categories for confirmed chemicals were explored to determine potential sources of contaminants.
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4. {"_":"Machine learning assisted identification of antibiotic-resistant strains using a paper-based ratiometric sensor array.","i":["Staphylococcus aureus"]}
4. {"_":"基于纸基比率传感器阵列的机器学习辅助抗生素耐药菌株鉴定。","i":["金黄色葡萄球菌"]}PMID:期刊:日期:2024-08-12, a versatile human pathogen, significantly impacts global health causing a broad spectrum of medical conditions that range from minor skin infections to life-threatening diseases. The clinical importance of is underscored by its resistance to multiple antibiotics and formation of biofilms, providing protection against antimicrobials and immune responses. To date, the identification of antimicrobial-resistant (AMR) strains, such as methicillin-resistant (MRSA) and vancomycin-intermediate (VISA), requires time-consuming and expensive methodologies, including culture-based, molecular, and phenotypic techniques. Previously, we developed a paper-based ratiometric sensor array composed of fluorescent sensor dyes (3-hydroxyflavone derivatives) pre-adsorbed on paper microzone plates. Combined with machine learning algorithms such as neural networks, this sensor effectively discriminated 16 bacterial species and determined their Gram status. In this study, we evaluate its ability to distinguish antibiotic-resistant strains and their biofilms. Our results demonstrate that the sensor array, in conjunction with LDA and neural networks, successfully differentiated three common laboratory MRSA strains from three methicillin-susceptible (MSSA) strains with 82.5% accuracy. Furthermore, using support vector machines, this sensor was able to distinguish and categorically classify MRSA, MSSA, and VISA clinical isolates with 97.5% accuracy. Remarkably, beyond distinguishing planktonic cultures, this sensor array demonstrated a formidable capability to discriminate AMR biofilms, achieving over 80% accuracy. Combined, the results of this study highlight the paper-based sensor array's significant potential as a robust diagnostic tool to accurately, rapidly, and easily identify drug-resistant strains in clinically relevant settings.
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5. Screening of monoamine oxidase B inhibitors in Tibetan strawberry by ligand fishing based on enzyme functionalized cellulose filter paper.
5. 基于酶功能化纤维素滤纸的配体垂钓技术筛选西藏草莓中的单胺氧化酶B抑制剂PMID:期刊:日期:2024-05-28Tibetan strawberry () is a wild medicinal and edible plant in Tibet possessing various health benefits such as neuroprotection and anti-oxidation. However, there has been little study reported on its chemical constituents. To investigate the inhibitors of monoamine oxidase B (MAO-B) in Tibetan strawberry, we immobilized the enzyme onto cellulose filter paper for the first time to develop a new screening method. Two known glycosides (compounds and ) and one new iridoid glucoside (Compound ) were fished out by this method, which was found to effectively inhibit MAO-B with IC values of 16.95 ± 0.93, 24.69 ± 0.20, and 46.77 ± 0.78 μM, respectively. Molecular docking and kinetic analysis were performed to reveal the inhibition mechanism of these compounds. Furthermore, compound exhibited neuroprotective effects against 6-OHDA-induced injury on PC12 cells. The developed method exhibits the advantages of rapidness and effectiveness in screening of MAO-B inhibitors from complex herbal extracts.
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6. Development of a sensitive direct competitive chemiluminescent enzyme immunoassay for gentamicin based on the construction of a specific single-chain variable fragment-alkaline phosphatase fusion protein.
6. 基于特异性单链可变区片段-碱性磷酸酶融合蛋白构建的灵敏直接竞争化学发光酶免疫分析法检测庆大霉素PMID:期刊:日期:2023-11-30A sensitive chemiluminescent enzyme immunoassay (CLEIA) was established for the determination of gentamicin (GEN) residue levels in animal tissue. This assay is based on a fusion protein of single-chain variable fragment (scFv) and alkaline phosphatase (AP). Initially, V and V derived from anti-gentamicin monoclonal antibody were linked by a short peptide to construct a scFv. Subsequently, the constructed scFv sequence was accessed into the pLIP6/GN vector, and a soluble scFv-AP fusion protein was generated. The scFv-AP fusion protein was used to develop a direct competitive CLEIA (dcCLEIA) for the determination of gentamicin. In the dcCLEIA, the half inhibitory concentration (IC) and limit of detection (LOD) were 1.073 ng/mL and 0.380 ng/mL, respectively. The average recoveries of gentamicin spiked in animal tissue samples ranged from 78% to 96%. These results showed a strong correlation with ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The above results suggest that the anti-GEN scFv-AP fusion protein is suitable for detecting gentamicin residues in edible animal tissues.
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7. A Portable, Encapsulated Microbial Whole-Cell Biosensing System for the Detection of Bioavailable Copper (II) in Soil.
7. 一种用于检测土壤中生物可利用铜（II）的便携式封装微生物全细胞生物传感系统PMID:期刊:日期:2023-07-17A portable, field deployable whole-cell biosensor was developed that can withstand the complex matrices of soil and requires minimal to no sample preparation to monitor bioavailable concentrations of the essential micronutrient copper (II). Conventional measurement of micronutrients is often complex, laboratory-based, and not suitable for monitoring their bioavailable concentration. To address this need, we developed a fluorescence based microbial whole-cell biosensing (MWCB) system encoding for a Cu-responsive protein capable of generating a signal upon binding to Cu. The sensing-reporting protein was designed by performing circular permutation on the green fluorescent protein (GFP) followed by insertion of a Cu binding motif into the structure of GFP. The design included insertion of several binding motifs and creating plasmids that encoded the corresponding sensing proteins. The signal generated by the sensing-reporting protein is directly proportional to the concentration of Cu in the sample. Evaluation of the resulting biosensing systems carrying these plasmids was performed prior to selection of the optimal fluorescence emitting Cu-binding protein. The resulting optimized biosensing system was encapsulated in polyacrylate-alginate beads and embedded in soil for detection of the analyte. Once exposed to the soil, the beads were interrogated to measure the fluorescence signal emitted by the sensing-reporting protein using a portable imaging device. The biosensor was optimized for detection of Cu in terms of selectivity, sensitivity, matrix effects, detection limits, and reproducibility in both liquid and soil matrices. The limit of detection (LoD) of the optimized encapsulated biosensor was calculated as 0.27 mg/L and 1.26 mg/kg of Cu for Cu in solution and soil, respectively. Validation of the portable imaging tools as a potential biosensing device in the field was performed.
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8. Two-Dimensional (2D) materials in the detection of SARS-CoV-2.
PMID:期刊:日期:2023-06-14The SARS-CoV-2 pandemic has resulted in a devastating effect on human health in the last three years. While tremendous effort has been devoted to the development of effective treatment and vaccines against SARS-CoV-2 and controlling the spread of it, collective health challenges have been encountered along with the concurrent serious economic impacts. Since the beginning of the pandemic, various detection methods like PCR-based methods, isothermal nucleic acid amplification-based (INAA) methods, serological methods or antibody tests, and evaluation of X-ray chest results have been exploited to diagnose SARS-CoV-2. PCR-based detection methods in these are considered gold standards in the current stage despite their drawbacks, including being high-cost and time-consuming procedures. Furthermore, the results obtained from the PCR tests are susceptible to sample collection methods and time. When the sample is not collected properly, obtaining a false result may be likely. The use of specialized lab equipment and the need for trained people for the experiments pose additional challenges in PCR-based testing methods. Also, similar problems are observed in other molecular and serological methods. Therefore, biosensor technologies are becoming advantageous with their quick response, high specificity and precision, and low-cost characteristics for SARS-CoV-2 detection. In this paper, we critically review the advances in the development of sensors for the detection of SARS-CoV-2 using two-dimensional (2D) materials. Since 2D materials including graphene and graphene-related materials, transition metal carbides, carbonitrides, and nitrides (MXenes), and transition metal dichalcogenides (TMDs) play key roles in the development of novel and high-performance electrochemical (bio)sensors, this review pushes the sensor technologies against SARS-CoV-2 detection forward and highlights the current trends. First, the basics of SARS-CoV-2 detection are described. Then the structure and the physicochemical properties of the 2D materials are explained, which is followed by the development of SARS-CoV-2 sensors by exploiting the exceptional properties of the 2D materials. This critical review covers most of the published papers in detail from the beginning of the outbreak.
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9. An Integrated Approach to Improve the Assay Performance of Quantum Dot-Based Lateral Flow Immunoassays by Using Silver Deposition.
PMID:期刊:日期:2023-06-07Traditional quantum dot-based lateral flow immunoassay (QD-LFIA) is limited to signal loss in part by the blinking, photobleaching and oxidative quenching of QD probes. Inspired by the good application of silver deposition on QD surfaces in tissue imaging, and in the context of improving the assay performance without compromising the simplicity and practicality, we report that introducing the QD-silver combination to the LFIA system, has the advantages of accuracy improvement, signal enhancement and user friendliness promotion, but maintains the cost-effective property and commercial accessibility of QD-LFIA. The effect was shown by using CdSe/ZnS QD-LFIA coupled with anti-sodium pentachlorophenate antibody, which provided a 4-fold improvement in the signal, a 2.5-fold improvement in the detection limit and a zero false-negative rate for sodium pentachlorophenate analysis in chicken samples. The proposed LFIA integrates the possibilities of colorimetric and fluorometric detection with different detection limits (fluorometric at 10 ng/mL and colorimetric at 4 ng/mL) and with acceptable detection times (fluorometric at 12 min and colorimetric at 27 min). The current results indicate that this QD-silver combined LFIA is complementary to conventional fluorescence LFIA and could be an inexpensive, versatile, and sensitive alternative.
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10. Synergistic effect of gold nanoparticles anchored on conductive carbon black as an efficient electrochemical sensor for sensitive detection of anti-COVID-19 drug Favipiravir in absence and presence of co-administered drug Paracetamol.
PMID:期刊:日期:2023-03-31Favipiravir (FVP) is introduced as a promising newly developed antiviral drug against the coronavirus disease 2019 (COVID-19). Therefore, the accurate determination of FVP is of great significance for quality assessment and clinical diagnosis. Herein, a novel electrochemical sensing platform for FVP based on gold nanoparticles anchored conductive carbon black (Au@CCB) modified graphite nanopowder flakes paste electrode (GNFPE) was constructed. Morphological and nanostructure properties of Au@CCB have been investigated by TEM, HRTEM, and EDX methods. The morphology and electrochemical properties of Au@CCB/GNFPE were characterized by SEM, cyclic voltammetry (CV), and EIS. The Au@CCB nanostructured modified GNFPE exhibited strong electro-catalytic ability towards the oxidation of FVP. The performance of the fabricated Au@CCB/GNFPE was examined by monitoring FVP concentrations in the absence and presence of co-administered drug paracetamol (PCT) by AdS-SWV. It was demonstrated that the proposed sensor exhibited superior sensitivity, stability, and anti-interference capability for the detection of FVP. The simultaneous determination of a binary mixture containing FVP and the co-administered drug PCT using Au@CCB/GNFPE sensor is reported for the first time. Under optimized conditions, the developed sensor exhibited sensitive voltammetric responses to FVP and PCT with low detection limits of 7.5 nM and 4.3 nM, respectively. The sensing electrode was successfully used to determine FVP and PCT simultaneously in spiked human plasma and pharmaceutical preparations, and the findings were satisfactory. Finally, the fabricated sensor exhibited high sensitivity for simultaneous detection of FVP and PCT in the presence of ascorbic acid in a real sample.