BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES生物化学与生物物理学报-生物膜
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES(英文缩写 BBA-BIOMEMBRANES),ISSN 0005-2736,eISSN 1879-2642,中文译名:生物化学与生物物理学报-生物膜 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.019 | Q2 |
| 2022 | 3.400 | Q2 |
| 2023 | 2.800 | Q2 |
| 2024 | 2.500 | Q3 |
| 2025 | 2.400 | Q3 |
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 最新收录文献
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1. Breakdown of classical paradigms in lipid membranes. Part II: The permeability paradox.
PMID:日期:2026-09-18Water soluble non electrolytes alter the density and the compressibility of the lipid membrane according to its ability to permeate. Glucose, a non-permeant, accumulates at the interphase binding to the PO2- groups promoting water extrusion and decreasing the specific volume and the compressibility without altering the vesicle size. Permeant as glycerol produces a lower change in agreement with its partial permeation. This behavior suggests a reduction in interfacial free volume and a restriction of area fluctuations while the membrane remains in the liquid-crystalline phase correlated with the shift of the distribution of water populations in PC membranes towards connected H bond populations. The polarization of the interphase increases from water to glucose congruent with an increase with the number of H-bonding groups reflected in changes in the water bands. Isotonic glycerol uptake presents a near-ideal Fickian diffusion mechanism, when vesicle swells due to displacement of glucose at the interface and expansion of the hydrated carbonyl population. In contrast, hypertonic permeation shows a non-Fickian transport regime (n = 1.16) due to the initial osmotic shrinkage. In this condition, the activation energy of permeation is lower than the isotonic one correlated to a ten-fold increase in unrelaxed water microstates, related to the exposure of hydrophobic regions and the creation of packing defects along the C=O plane. The results validate a model of membrane interphase of hydrated headgroups dissolved in loose, labile water providing a powerful framework for the intelligent design of smart liposomal controlled delivery systems triggered by solute-induced release.
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2. Modes of interaction between tapinarof and model lipid membranes mimicking stratum corneum lipid composition and related transdermal transport in human skin.
2. 苯维莫德与模拟角质层脂质组成的模型脂质膜之间的相互作用模式及在人皮肤中相关的透皮转运PMID:日期:2026-09-10Tapinarof (3,5-dihydroxy-4-isopropylstilbene, TPN), structurally related to resveratrol, is a nonsteroidal agent that belongs to the class of naturally derived aryl hydrocarbon receptor (AhR) agonists. TPN is an active ingredient of a topical medication (1% cream, VTAMA®). The log P of 2.79 ± 0.08 confirms the relative lipophilic character of TPN. The confirmed hydrophobicity of TPN raises the question of how this drug interacts with model lipidic structures and cell membranes, providing information about its transport mechanisms in the skin. Based on a liposomal model (40 % (w/w) ceramide, 25 % cholesterol, 25 % lignoceric acid, and 10 % cholesterol sulphate), it was shown that TPN causes a significant increase in fluidity and possibly a disruption of the membrane. The investigated 50-100 nm unilamellar liposomes were strictly negatively charged (zeta potential approx. -85 mV) and mimic skin lipid composition of the stratum corneum. The disruption mechanism is discussed in terms of the intermolecular interactions between TPN molecules (aggregates) and the various lipids constituting the liposomal model. Molecular dynamic simulations revealed rapid insertion of TPN into the lipid bilayer at low concentrations, whereas higher concentrations promoted TPN self-association and membrane perturbation, characterized by decreased lipid order and membrane thickness together with increased area per lipid. Finally, we confirmed a dual behaviour of TPN ex vivo: it can accumulate in the human skin, but it is also sufficiently mobile to partially cross this barrier.
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3. Self-assembled cationic lipopeptides modulate membrane interactions, cellular uptake, and mitochondrial dysfunction in HeLa cells.
PMID:日期:2026-09-07Self-assembled cationic lipopeptides are membrane-active amphiphilic systems whose biological responses depend on molecular composition and supramolecular organization. Here, we investigated the structure-dependent cytotoxicity, hemocompatibility, cellular association/internalization, and cell-death mechanisms of four cationic lipopeptides with distinct peptide sequences and lipidation patterns: arginine-containing PRWG derivatives and guanidinocarbonylpyrrole (GCP)-containing PK(GCP)WG derivatives. Cytotoxicity assays showed concentration-dependent reductions in cell viability, with cell-line-dependent responses between HeLa and HEK293 cells. The double-lipidated GCP-containing lipopeptide showed the most favorable differential viability trend between these cell lines under the tested conditions, whereas PRWG-based lipopeptides induced more pronounced apoptosis-associated responses in HeLa cells. Hemolysis assays showed low hemolytic activity after 3 h, supporting preliminary short-term hemocompatibility; however, 24 h exposure revealed concentration- and structure-dependent hemolysis, particularly for PRWG-CH. Cellular association/internalization studies using coumarin-6 as a physically incorporated hydrophobic fluorescent probe revealed higher cell-associated fluorescence in HeLa cells than in HEK293 cells, suggesting that membrane interactions contribute to the biological response. Because coumarin-6 was physically incorporated rather than covalently attached, these data should be interpreted as cell-associated fluorescence from coumarin-6-loaded assemblies rather than direct quantitative evidence of intact lipopeptide uptake. Annexin V/7-AAD staining and JC-1 analysis indicated that PRWG-based lipopeptides predominantly induced apoptosis-associated cell death and mitochondrial membrane depolarization in HeLa cells, particularly the double-lipidated PRWG derivative. These findings show that peptide sequence, lipidation pattern, apparent surface charge, supramolecular morphology, and membrane-associated fluorescence collectively modulate the biological activity of cationic lipopeptides, highlighting their relevance as supramolecular platforms for investigating tunable lipopeptide-membrane interactions and cell-death responses.
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4. Sequence-dependent transferability of the LRLLR membrane translocation motif: A computational study of smacN and NR2B9c peptides.
4. LRLLR膜转位基序依赖序列的转位能力：smacN和NR2B9c肽的计算研究PMID:日期:2026-09-05The LRLLR cell-penetrating motif can be transferred to confer membrane translocation activity, but only to compatible recipient peptides. Using umbrella sampling molecular dynamics simulations, we show that C-terminal LRLLR addition to the pro-apoptotic smacN peptide eliminates its translocation barrier, transforming a + 65 kJ/mol barrier into a - 50 kJ/mol energy well. In contrast, N-terminal LRLLR addition to the neuroprotective NR2B9c peptide increases the barrier from +85 to +100 kJ/mol. The LRLLR pentapeptide, identified through systematic screening as essential for spontaneous translocation, represents a minimal penetrating element whose transferability was unknown. We appended this motif to both peptides and calculated potential of mean force profiles across a POPC/POPG bilayer. Analysis of hydrogen bonding patterns, secondary structure, and conformational dynamics reveals the structural basis for these divergent outcomes. Successful transfer to smacN reflects favorable charge complementarity: the hydrophobic, neutral AVPI tetrapeptide provides an ideal platform for the charged, amphipathic LRLLR motif, enabling simultaneous interaction with both membrane leaflets. Transfer failure with NR2B9c stems from its positive charge and conformational rigidity imposed by intramolecular hydrogen bonding, preventing optimal membrane insertion. These findings establish that CPP motif transfer requires compatibility in charge distribution, hydrophobicity, and conformational flexibility, and demonstrate the value of computational screening to identify compatible motif-cargo pairings prior to experimental investment.
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5. Mass photometry and its expanding role in membrane protein research: The Torpedo marmorata nicotinic acetylcholine receptor as case study.
PMID:日期:2026-09-03Integral membrane proteins (IMPs) are challenging, yet highly relevant targets for structural biology. Selection of the appropriate membrane mimic environment necessitates extensive optimization using a combination of biochemical and biophysical techniques. We report here on the expanding role of mass photometry (MP) as an in-solution technique to enable single-particle analysis of IMPs. To showcase MP versatility, we present a selection of examples of MP applications for IMP analysis, using nicotinic acetylcholine receptor (nAChR) extracted from Torpedo marmorata as case study, together with additional literature examples. We further illustrate how MP supports the screening of purification conditions, and the quality control of samples prior to cryo-electron microscopy grid preparation or native mass spectrometry. The benefits of MP for IMP characterization are illustrated through its ability to quantify single-molecule populations, study membrane biomolecular assemblies, and characterize membrane-associated phenomena from very low amounts of starting material. MP therefore stands out as a unique biophysical tool that combines rapid analysis with broad adaptability to most solubilizing environments, enabling simultaneous relative quantification of all detected species in a highly user-friendly manner.
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6. Calix[4]arene C-1220 - Modulator of calcium homeostasis and contractile activity in smooth muscle cells.
PMID:日期:2026-09-01Regulation of Na,K-ATPase activity plays a critical role in maintaining ionic homeostasis and Ca-dependent contractility in smooth muscle, yet the contribution of selective sodium pump modulators to myometrial function remains insufficiently understood. In this study, we investigated the effects of calix[4]arene C-1220 (25,27-dipropoxycalix[4]arene-bis-N-tolylsulfonylaminomethylphosphonic acid) and its structural analogue C-99 on ATP-hydrolyzing activities, intracellular Ca dynamics, mitochondrial function, and contractile behavior of uterine smooth muscle. Enzymatic assays showed that C-1220 is a highly potent and selective inhibitor of Na,K-ATPase (IC₅₀ = 48 ± 2 nM) without influencing other ATPases in the plasma membrane. Confocal imaging demonstrated that C-1220 elevates cytosolic Ca in myocytes, whereas flow cytometry and Ca-accumulation assays indicated that mitochondrial membrane polarization and matrix Ca levels remain unaffected. Tensometric measurements revealed that both C-1220 and C-99 enhance spontaneous contractions and force-, velocity-, and impulse-related mechanokinetic parameters; however, normalization of velocity parameters suggests that these changes do not reflect direct modulation of Ca-transporting systems. Comparative analysis supports the conclusion that inhibition of Na,K-ATPase and consequent disturbance of Na/Ca exchange underlie the contractile activation induced by both compounds. These findings provide new insights into membrane mechanisms governing electro- and pharmacomechanical coupling in uterine smooth muscle and highlight C-1220 as a useful molecular tool for probing Na,K-ATPase-dependent Ca regulation.
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8. Composition-tunable H-PC/LPC discoidal lipid assemblies: molecular packing, nanoscale organization, and permeation behavior.
PMID:日期:2026-08-26Discoidal membrane-like lipid assemblies provide a useful framework for linking lipid composition to nanoscale organization and interfacial transport, but practical solvent-avoiding routes from formulation-compatible phospholipids remain limited. Here, hydrogenated phosphatidylcholine (H-PC)/lysophosphatidylcholine (LPC) dispersions were prepared in the presence of 1,3-butylene glycol (BG) by a simple heating-stirring procedure that avoids organic solvents used in thin-film hydration. Varying the H-PC/LPC ratio revealed a composition window in which transparent nanoscale dispersions with apparent hydrodynamic diameters of 10-20 nm were obtained. TEM and P NMR analyses most clearly supported discoidal assemblies with bicelle-like features at intermediate LPC fractions, whereas lower LPC fractions yielded lamellar/vesicular aggregates and higher LPC fractions showed smaller, more isotropic aggregate states. DSC showed a decrease in the main endothermic transition temperature with increasing LPC fraction, consistent with composition-dependent thermotropic phase behavior and altered packing compatibility between H-PC-rich ordered domains and LPC-rich components. In Franz diffusion experiments using caffeine as a hydrophilic probe, H-PC/LPC dispersions showed higher permeation across Strat-M® membranes than a BG-matched lipid-free control. Compared with LPC-only formulations, the mixed H-PC/LPC series showed a clearer composition-dependent permeation profile, indicating that permeation behavior is associated with the nanoscale state of the mixed lipid dispersion rather than LPC content alone. A representative discoidal-assembly composition gave a numerically higher cumulative permeated amount across excised human skin. These results identify a practical route to composition-tunable H-PC/LPC lipid assemblies with discoidal, bicelle-like features and show that molecular packing and aggregate organization are linked to permeation behavior at biological interfaces.
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9. Parasitophorous vacuole membranes of Toxoplasma gondii and Plasmodium falciparum lack the lipid asymmetry characteristic of host cell plasma membranes.
9. 弓形虫和恶性疟原虫的寄生泡膜缺乏宿主细胞质膜所具有的脂质不对称性特征PMID:日期:2026-08-14Apicomplexan parasites, including Toxoplasma gondii and Plasmodium falciparum, reside within a specialized compartment known as the parasitophorous vacuole (PV) during their intracellular life cycle. The PV membrane (PVM), which derives from the host plasma membrane upon invasion, serves as a selective barrier that permits nutrient acquisition while shielding the parasite from host defense mechanisms. Although the protein composition of the PVM has been studied extensively, its lipid organization remains poorly understood. Using the quick-freeze, freeze-fracture replica labeling (QF-FRL) method, we quantitatively analyzed the transbilayer distribution of phosphatidylserine (PtdSer), phosphatidylethanolamine (PtdEtn), and GM3 ganglioside in the PVM of T. gondii and P. falciparum. Unlike host cell plasma membranes, where these lipids exhibit strict asymmetry-PtdSer and PtdEtn confined to the cytoplasmic leaflet and GM3 to the exoplasmic leaflet-we found that all three lipids were symmetrically distributed across both leaflets of the PVM. This striking loss of lipid asymmetry suggests that the PVM undergoes profound remodeling during infection. The presence of PtdSer and PtdEtn in the luminal leaflet may facilitate the binding of perforin-like proteins (PLP1s) during egress. These findings reveal a unique feature of the PVM that redefines our understanding of host-parasite membrane biology.
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10. Topological mapping establishes signal peptide-independent cytoplasmic membrane insertion of bacterial MATE family efflux protein EmmdR.
PMID:日期:2026-08-10Enterobacter cloacae ATCC13047 encodes the EmmdR efflux protein, which belongs to the Multidrug And Toxic compound Extrusion (MATE) transporter family (Transporter Classification ID 2.A.66.1). The protein has a predicted existence in the Uniprot database owing to the lack of structure elucidation and experimental evidence of membrane localization. This study experimentally validated the predicted topology of EmmdR and ascertained the role of charged amino acids in membrane insertion. The experimental strategy included qualitative and quantitative assays of dual reporter PhoA-LacZ enzymes coupled with microscopic analysis to ascertain the localization of EmmdR. The topology of periplasmic loop 1 was confirmed by immunofluorescence analysis. The results of this study corroborated the predicted 12 transmembrane segment architecture of EmmdR. Colocalization of green fluorescent protein (GFP) with membrane staining dye FM4-64 confirmed cytoplasmic membrane localization and cytoplasmic orientation of C and N-terminus of EmmdR. Immunofluorescence microscopy of spheroplasts confirmed the periplasmic localization of loop 1. The membrane insertion of EmmdR concurred with the 'positive inside' rule, and deletion of critical positive amino acids in the cytoplasmic loop resulted in altered topology. Thus, this study is the first to report the experimental topology determination and membrane localization of EmmdR, which validates the predicted existence and supports the predicted structure of EmmdR.