点击化学修饰的心肌细胞增强心脏收缩力并预防缺血再灌注损伤心脏的重构
Click chemistry-modified cardiomyocytes enhance cardiac contractility and prevent remodeling in ischemia-reperfusion injured hearts.
摘要(中文译文)
利用人多能干细胞来源的心肌细胞（hPSC-CMs）进行心脏细胞治疗面临的挑战之一，是其在梗死组织中植入率和存活率较低。点击化学和脂质插入是两种简便的方法，可利用胶原杂交肽（CHP）修饰hPSC-CMs表面，提供结合基序以提高其在缺血-再灌注（I/R）损伤后纤维化心脏中的滞留和植入。在本研究中，我们开发了CHP修饰的心肌细胞（CHP-mCMs），使细胞附着于梗死组织中受损的胶原。我们在心肌梗死后7天将CHP-mCMs注射到梗死心脏的边缘区，并评估心脏功能6周。系列超声心动图显示，CHP-mCMs不仅改善了心脏功能，还减少了I/R损伤心脏的不良重构。这些发现表明，在大鼠I/R模型中，CHP-mCM移植与植入增强、不良重构减少及心脏功能改善相关。
Abstract
One of the challenges in cardiac cell therapy using cardiomyocytes derived from human pluripotent stem cells (hPSC-CMs) is their poor engraftment and survival rate in infarcted tissues. Click chemistry and lipid insertion are simple methods for modifying the surface of hPSC-CMs with a collagen hybridizing peptide (CHP), providing binding motifs to increase their retention and engraftment in fibrotic hearts after ischemia-reperfusion (I/R) injury. In this study, we developed CHP-modified CMs (CHP-mCMs) to attach cells to damaged collagen in infarcted tissues. We injected CHP-mCMs into the border zone of infarcted hearts 7 days post-MI and evaluated cardiac function for 6 weeks. Serial echocardiography revealed that CHP-mCMs not only improved cardiac function but also reduced adverse remodeling in I/R-injured hearts. These findings indicate that CHP-mCM transplantation was associated with enhanced engraftment, reduced adverse remodeling, and improved cardiac function in the rat I/R model.
如何引用
AMA
Donghyun Lee, Woo-Sup Sim, Jae-Hyun Park, Dong-Yeon Han, Soo-Jeong Kim, Jin Ryeol An, et al. Click chemistry-modified cardiomyocytes enhance cardiac contractility and prevent remodeling in ischemia-reperfusion injured hearts.. Bioactive materials. 2027; doi:10.1016/j.bioactmat.2026.08.016.
APA
Donghyun Lee, Woo-Sup Sim, Jae-Hyun Park, Dong-Yeon Han, Soo-Jeong Kim, Jin Ryeol An, et al (2027). Click chemistry-modified cardiomyocytes enhance cardiac contractility and prevent remodeling in ischemia-reperfusion injured hearts.. Bioactive materials. https://doi.org/10.1016/j.bioactmat.2026.08.016
GB/T 7714
Donghyun Lee, Woo-Sup Sim, Jae-Hyun Park, Dong-Yeon Han, Soo-Jeong Kim, Jin Ryeol An, et al. Click chemistry-modified cardiomyocytes enhance cardiac contractility and prevent remodeling in ischemia-reperfusion injured hearts.[J]. Bioactive materials, 2027 doi:10.1016/j.bioactmat.2026.08.016.
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