具有组织匹配力学性能的可编程4D打印可持续多功能光敏树脂用于界面组织再生
Programmable 4D printing of sustainable multifunctional photoresins with tissue-matched mechanics for interfacial tissue regeneration.
摘要(中文译文)
关节软骨与软骨下骨的整合性再生仍是一项重大临床挑战，原因在于人工植入物难以模拟天然骨软骨组织的空间与组成复杂性。为克服这些局限，3D打印实现了个性化解决方案，超越了仅限于修复小型圆柱形局灶性缺损的传统柱状支架。在本研究中，我们报道了一种用于骨软骨组织工程应用的无溶剂4D打印橄榄油衍生脂肪酰胺基丙烯酸酯光敏树脂。通过使用丙烯酸作为共聚单体，对打印聚合物的理化性质进行了精细调控。在优化打印条件下，该树脂可打印成高分辨率物体，每层约50-100 μm。根据树脂组成的不同，打印聚合物的力学性能在拉伸条件下约为200-1400 kPa，应变约为210-420%；在压缩条件下约为26-98 MPa，应变约为80%。打印聚合物的力学性能与多种界面软组织相当，包括韧带、关节软骨和软胶原骨，表明其在界面组织工程应用中具有潜力。此外，打印聚合物表现出接近体温响应性的形状记忆（4D）特性，优化树脂组成的恢复率约为99%。此外，打印聚合物对革兰氏阴性菌和革兰氏阳性菌均表现出优异的杀菌抗菌活性，凸显了其多功能特性。更重要的是，打印聚合物对脂肪来源的人间充质干细胞表现出良好的细胞相容性（与对照相比，所有样本的细胞活力约≥90%），促进了无生长因子的成骨和成软骨分化，证实了其在骨软骨组织再生中的潜力。总体而言，这种新开发的生物基树脂为骨软骨组织再生的个性化植入物4D打印提供了一种低成本、可持续的替代方案。
Abstract
The integrative regeneration of articular cartilage and subchondral bone remains a major clinical challenge due to the difficulties of mimicking the spatial and compositional complexity of native osteochondral tissues in artificial implants. To overcome these limitations, 3D printing has enabled personalized solutions, going beyond traditional plug-shaped scaffolds that are limited to repairing small, cylindrical focal defects. In this study, we report solvent-free 4D printing of olive oil-derived fatty amide-based acrylate photoresin for osteochondral tissue engineering applications. The physicochemical properties of the printed polymers were fine-tuned using acrylic acid as a comonomer. With optimized printing conditions, the resin can be printed into high-resolution objects with each layer of ∼50-100 μm. Depending on the resin composition, the mechanical properties of the printed polymers vary between ∼200-1400 kPa with ∼210-420% strain under tensile setting, and ∼26-98 MPa at ∼80% strain under compressive setting. The mechanical properties of the printed polymers are comparable to those of various interfacial soft tissues, including ligaments, articular cartilage, and soft collagenous bone, indicating their potential for interfacial tissue engineering applications. Furthermore, the printed polymers showed near-body temperature-responsive shape memory (4D) properties with a recovery ratio of ∼99% for optimized resin composition. In addition, the printed polymers exhibited excellent bactericidal antimicrobial activity against both Gram-negative and Gram-positive bacteria, highlighting their multifunctional nature. More importantly, the printed polymers exhibited good cytocompatibility (cell viability ∼≥90% across all samples compared to control) toward adipose-derived human mesenchymal stem cells, facilitating growth factor-free osteogenic and chondrogenic differentiation, which confirms their potential for osteochondral tissue regeneration. Overall, the newly developed biobased resin offers a low-cost, sustainable alternative for 4D printing of personalized implants for osteochondral tissue regeneration.
如何引用
AMA
Krishanu Ghosal, Qi Wu, Ashis Ghosh, Ranabir Majumder, Mahitosh Mandal, Shady Farah. Programmable 4D printing of sustainable multifunctional photoresins with tissue-matched mechanics for interfacial tissue regeneration.. Bioactive materials. 2027; doi:10.1016/j.bioactmat.2026.06.017.
APA
Krishanu Ghosal, Qi Wu, Ashis Ghosh, Ranabir Majumder, Mahitosh Mandal, Shady Farah (2027). Programmable 4D printing of sustainable multifunctional photoresins with tissue-matched mechanics for interfacial tissue regeneration.. Bioactive materials. https://doi.org/10.1016/j.bioactmat.2026.06.017
GB/T 7714
Krishanu Ghosal, Qi Wu, Ashis Ghosh, Ranabir Majumder, Mahitosh Mandal, Shady Farah. Programmable 4D printing of sustainable multifunctional photoresins with tissue-matched mechanics for interfacial tissue regeneration.[J]. Bioactive materials, 2027 doi:10.1016/j.bioactmat.2026.06.017.
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