黑果枸杞中的异鼠李素通过调控氧化应激、线粒体功能和AKT信号通路发挥抗炎和抗癌活性
{"_":"Isorhamnetin from Murr. exhibits anti-inflammatory and anticancer activities via regulation of oxidative stress, mitochondrial function, and AKT signaling.","i":["Lycium ruthenicum","in vitro"]}
摘要(中文译文)
黑果枸杞（Lycium ruthenicum Murr.）因其免疫调节和抗癌潜力而受到关注，但其关键活性成分及 underlying 机制仍不清楚。本研究采用液相色谱-串联质谱（LC-MS/MS）对黑果枸杞的代谢物谱进行表征，并通过体外实验评估其抗炎和抗癌活性。共鉴定出149种代谢物，以有机酸、碳水化合物和氨基酸衍生物为主要成分。异鼠李素（isorhamnetin）被鉴定为代表性的黄酮类化合物。在脂多糖（LPS）刺激的Calu3细胞中，异鼠李素减少了促炎细胞因子（IL-6、TNF-α、IL-1β）的分泌及mRNA表达。在癌细胞中，异鼠李素诱导ROS蓄积、DNA损伤、线粒体功能障碍以及caspase-3依赖性凋亡，并伴随对AKT信号通路的调控。这些发现提示，异鼠李素可能有助于黑果枸杞所观察到的抗炎和抗癌作用，为其作为功能性食品成分的潜力提供了初步实验证据。然而，本研究受限于仅使用单一细胞系（Calu3）以及缺乏体内验证和药代动力学评价；尚需进一步研究以证实这些观察结果。
Abstract
Black goji berry ( Murr.) has been recognized for its immune-regulating and anti-cancer potentials, but its key active components and underlying mechanisms remain unclear. Here, we applied LC-MS/MS to characterize the metabolite profile of and performed assays to evaluate its anti-inflammatory and anti-cancer activities. A total of 149 metabolites were identified, with organic acids, carbohydrates, and amino acid derivatives as the dominant components. Isorhamnetin was identified as a representative flavonoid. In LPS-stimulated Calu3 cells, isorhamnetin reduced the secretion and mRNA expression of pro-inflammatory cytokines (IL-6, TNF-alpha, IL-1beta). In cancer cells, isorhamnetin induces ROS accumulation, DNA damage, mitochondrial dysfunction, and caspase-3-dependent apoptosis, with modulation of the AKT signaling pathway. These findings suggest that isorhamnetin may contribute to the anti-inflammatory and anti-cancer effects of observed , providing preliminary experimental evidence for its potential as a functional food ingredient. However, this study is limited by the use of a single cell line (Calu3) and the absence of validation and pharmacokinetic evaluation; further studies are needed to confirm these observations.
如何引用
AMA
Lei Jiang, Jideng Ma, Zhiyuan Li, Yuqi Li, Shihan Zhang, Huifeng Li. {"_":"Isorhamnetin from Murr. exhibits anti-inflammatory and anticancer activities via regulation of oxidative stress, mitochondrial function, and AKT signaling.","i":["Lycium ruthenicum","in vitro"]}. Frontiers in nutrition. 2026; doi:10.3389/fnut.2026.1750263.
APA
Lei Jiang, Jideng Ma, Zhiyuan Li, Yuqi Li, Shihan Zhang, Huifeng Li (2026). {"_":"Isorhamnetin from Murr. exhibits anti-inflammatory and anticancer activities via regulation of oxidative stress, mitochondrial function, and AKT signaling.","i":["Lycium ruthenicum","in vitro"]}. Frontiers in nutrition. https://doi.org/10.3389/fnut.2026.1750263
GB/T 7714
Lei Jiang, Jideng Ma, Zhiyuan Li, Yuqi Li, Shihan Zhang, Huifeng Li. {"_":"Isorhamnetin from Murr. exhibits anti-inflammatory and anticancer activities via regulation of oxidative stress, mitochondrial function, and AKT signaling.","i":["Lycium ruthenicum","in vitro"]}[J]. Frontiers in nutrition, 2026 doi:10.3389/fnut.2026.1750263.
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