基于自组装磁性探针的双模式现场可部署平台，用于同时监测基孔肯雅病毒和宿主免疫状态
Dual-Mode Field-Deployable Platform Based on Self-Assembled Magnetic Probes for Simultaneous Monitoring of Chikungunya Virus and Host Immune Status.
摘要(中文译文)
病原体载量与宿主免疫反应的实时双重监测有助于风险分层和精准治疗，但在技术上仍具有挑战性。针对基孔肯雅热疫情控制的需求，本研究开发了一种柔性、超灵敏的双模式侧向流免疫分析（LFA），能够在临床样本中同时检测基孔肯雅病毒（CHIKV）抗原和炎症细胞因子IL-6。通过静电自组装合成了增强型比色-荧光双信号磁性探针（MAu2QD3）。通过调节负载信号层（Au和CdSe/ZnS颗粒）的数量，该LFA平台实现了双信号输出强度的可控调节。同时，构建了基于人工智能的双模式定量模型，可自动整合比色和荧光读数，从而获得准确的靶标浓度估计。在裸眼模式下，该检测对CHIKV和IL-6的视觉检测限（LOD）均为50 pg·mL-1。在便携式仪器辅助下，MAu2QD3-LFA的比色灵敏度至少提高4倍，而荧光检测进一步将CHIKV的LOD降至0.28 pg·mL-1，IL-6的LOD降至0.16 pg·mL-1。使用91份临床样本进行验证，该系统实现了98.2%的灵敏度和100%的特异性，能够可靠地获取病原体和宿主免疫信息。这些结果凸显了其在传染病早期监测和精准诊断方面的强大潜力。
Abstract
Real-time dual monitoring of pathogen burden and host immune response enables risk stratification and precision therapy, yet remains technically challenging. Addressing the needs of chikungunya fever outbreak control, here, we developed a flexible, ultrasensitive dual-mode lateral flow immunoassay (LFA) capable of simultaneous detection of chikungunya virus (CHIKV) antigen and the inflammatory cytokine IL-6 across clinical samples. An enhanced colorimetric-fluorescent dual-signal magnetic probe (MAu2QD3) was synthesized via electrostatic self-assembly. By tuning the number of loaded signal layers (Au and CdSe/ZnS particles), the LFA platform achieved controllable adjustment of dual-signal output intensities. In parallel, an artificial intelligence-based dual-mode quantitative model was built to automatically integrate colorimetric and fluorescent readouts, yielding accurate target concentration estimates. In naked-eye mode, the assay exhibited visual limits of detection (LODs) of 50 pg·mL-1 for both CHIKV and IL-6. With portable instrument assistance, the colorimetric sensitivity of MAu2QD3-LFA increased by at least 4-fold, while fluorescent detection further reduced the LODs to 0.28 pg·mL-1 for CHIKV and 0.16 pg·mL-1 for IL-6. Validated using 91 clinical samples, the system achieved a sensitivity of 98.2% and a specificity of 100%, enabling reliable acquisition of both pathogen and host immune information. These results highlight strong potential for early surveillance and precise diagnosis of infectious diseases.
如何引用
AMA
Junyan Ai, Jiaxuan Li, Qian Wang, Tao Zhang, Meirou Lu, Jiahuan Zhang, et al. Dual-Mode Field-Deployable Platform Based on Self-Assembled Magnetic Probes for Simultaneous Monitoring of Chikungunya Virus and Host Immune Status.. ACS applied materials & interfaces. 2026; doi:10.1021/acsami.6c10007.
APA
Junyan Ai, Jiaxuan Li, Qian Wang, Tao Zhang, Meirou Lu, Jiahuan Zhang, et al (2026). Dual-Mode Field-Deployable Platform Based on Self-Assembled Magnetic Probes for Simultaneous Monitoring of Chikungunya Virus and Host Immune Status.. ACS applied materials & interfaces. https://doi.org/10.1021/acsami.6c10007
GB/T 7714
Junyan Ai, Jiaxuan Li, Qian Wang, Tao Zhang, Meirou Lu, Jiahuan Zhang, et al. Dual-Mode Field-Deployable Platform Based on Self-Assembled Magnetic Probes for Simultaneous Monitoring of Chikungunya Virus and Host Immune Status.[J]. ACS applied materials & interfaces, 2026 doi:10.1021/acsami.6c10007.
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