在晶体管上具有级联运算逻辑的DNA纳米桥，用于呼吸系统感染的综合评估
DNA Nanobridge with Cascade Operation Logic on a Transistor for Comprehensive Evaluation of Respiratory Infection.
摘要(中文译文)
急性呼吸道感染可能引发细胞因子风暴，对生命构成重大威胁。因此，现场病原体筛查和感染严重程度评估对于即时医疗干预极为必要。在此，我们报道了通过石墨烯场效应晶体管（g-FET）对呼吸道感染进行综合监测，该晶体管经DNA纳米桥修饰，纳米桥由两个DNA四面体通过桥链连接而成。靶标病毒核酸的识别触发桥链解离，释放探针以检测炎症细胞因子IL-6。这种级联逻辑操作能够同时检测呼吸道病原体并评估相关的炎症反应。该器件可检测低至3.9 × 10 M的病毒核酸，而临床相关水平2.4 × 10 M的IL-6存在使响应增强3.7倍。因此，对临床COVID-19口咽拭子的检测与PCR结果达到约97.2%的总体一致性，在实际应用中展现出巨大潜力。
Abstract
Acute respiratory infections may cause a cytokine storm that poses a great threat to life. Thus, on-site pathogen screening and infection severity assessment are highly desired for immediate medical intervention. Here, we report the comprehensive monitoring of respiratory infection by a graphene field-effect transistor (g-FET) modified with a DNA nanobridge that consists of two DNA tetrahedrons linked by a bridge strand. The recognition of target viral nucleic acid triggers bridge dissociation, releasing the probe to detect the inflammatory cytokine IL-6. Such a cascade logical operation enables simultaneous detection of respiratory pathogens and evaluation of the associated inflammatory response. The device detects viral nucleic acid as low as 3.9 × 10 M, while the presence of IL-6 at a clinically relevant level of 2.4 × 10 M enhances the response by 3.7-fold. Thus, testing of clinical COVID-19 oropharyngeal swabs achieves ∼97.2% overall agreement with PCR results, demonstrating immense potential in practical applications.
如何引用
AMA
Xiangyu Lv, Yanan Tang, Yang Yue, Mengzhu Wang, Chang Chen, Zihao Chen, et al. DNA Nanobridge with Cascade Operation Logic on a Transistor for Comprehensive Evaluation of Respiratory Infection.. ACS applied materials & interfaces. 2026; doi:10.1021/acsami.6c05125.
APA
Xiangyu Lv, Yanan Tang, Yang Yue, Mengzhu Wang, Chang Chen, Zihao Chen, et al (2026). DNA Nanobridge with Cascade Operation Logic on a Transistor for Comprehensive Evaluation of Respiratory Infection.. ACS applied materials & interfaces. https://doi.org/10.1021/acsami.6c05125
GB/T 7714
Xiangyu Lv, Yanan Tang, Yang Yue, Mengzhu Wang, Chang Chen, Zihao Chen, et al. DNA Nanobridge with Cascade Operation Logic on a Transistor for Comprehensive Evaluation of Respiratory Infection.[J]. ACS applied materials & interfaces, 2026 doi:10.1021/acsami.6c05125.
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