原位形成深共晶溶剂，用于食品和药品样品中Fast Green和红霉素的无分散微萃取
In situ formation of deep eutectic solvent for disperser-free microextraction of Fast Green and Erythrosine in food and pharmaceutical samples.
摘要(中文译文)
提出了一种快速、环境友好的原位深共熔溶剂（DES）基液-液微萃取方法，用于食品和药物样品中固绿（FG）和赤藓红（ER）的预富集与测定。该DES由百里香酚与四丁基溴化铵（1:2）组成，可原位合成，无需分散液或预先制备。对pH、DES比例、萃取时间、温度、离子强度和离心时间等关键萃取参数进行了优化，以实现最大萃取效率。采用傅里叶变换红外光谱（FT-IR）和紫外-可见光谱研究了染料与DES之间的相互作用机制。在最优条件下，该方法对FG在30-1200 µg L⁻¹范围内、对ER在48-2000 µg L⁻¹范围内呈现良好线性，FG和ER的检出限分别为9.0和13.9 µg L⁻¹。该方法具有高精密度（RSD < 4%），在加标样品中获得了满意的回收率（97.5-99.9%）。采用AGREEprep和Click Analytical Chemistry Index工具对该方法的绿色度及适用性进行了评估。
Abstract
A rapid, environmentally friendly, in situ deep eutectic solvent (DES) based liquid-liquid microextraction method was proposed for the preconcentration and determination of Fast Green (FG) and Erythrosine (ER) in food and pharmaceutical samples. The DES, which was synthesized in situ without the need for disperser liquids or prior preparation, was composed of thymol and tetrabutylammonium bromide (1:2). Key extraction parameters, including pH, DES ratio, extraction time, temperature, ionic strength, and centrifugation time, were adjusted to achieve maximum efficiency. The interaction mechanism between the dyes and DES was investigated using FT-IR and UV-Visible spectroscopy. Under optimal conditions, the method showed good linearity over 30-1200 µg L⁻¹ for FG and 48-2000 µg L⁻¹ for ER, with detection limits of 9.0 and 13.9 µg L⁻¹ for FG and ER, respectively. The method exhibited high precision (RSD < 4%) and satisfactory recoveries (97.5-99.9%) in spiked samples. Greenness assessment and applicability of the method were assessed using AGREEprep and Click Analytical Chemistry Index tools.
如何引用
AMA
Fatma T Abdelwahed, Wael I Mortada, Nourhan N Hassan, Rania M Eltabey. In situ formation of deep eutectic solvent for disperser-free microextraction of Fast Green and Erythrosine in food and pharmaceutical samples.. Journal of chromatography. A. 2026; doi:10.1016/j.chroma.2026.467274.
APA
Fatma T Abdelwahed, Wael I Mortada, Nourhan N Hassan, Rania M Eltabey (2026). In situ formation of deep eutectic solvent for disperser-free microextraction of Fast Green and Erythrosine in food and pharmaceutical samples.. Journal of chromatography. A. https://doi.org/10.1016/j.chroma.2026.467274
GB/T 7714
Fatma T Abdelwahed, Wael I Mortada, Nourhan N Hassan, Rania M Eltabey. In situ formation of deep eutectic solvent for disperser-free microextraction of Fast Green and Erythrosine in food and pharmaceutical samples.[J]. Journal of chromatography. A, 2026 doi:10.1016/j.chroma.2026.467274.
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