JOURNAL OF CHROMATOGRAPHY A色谱学杂志A辑
JOURNAL OF CHROMATOGRAPHY A(英文缩写 J CHROMATOGR A),ISSN 0021-9673,eISSN 1873-3778,中文译名:色谱学杂志A辑 是一本学术期刊。本页汇总该期刊的最新影响因子、分区信息以及最新收录于 PubMed 的文献,帮助您快速了解期刊全貌。
发文量统计区间：2025-11-01 至 2026-10-31，按本站收录文献的发表日期统计。
期刊介绍
历年影响因子趋势
| JCR 数据年份 | 影响因子 | JCR 分区 |
|---|---|---|
| 2021 | 4.601 | Q1 |
| 2022 | 4.100 | Q2 |
| 2023 | 3.800 | Q1 |
| 2024 | 4.000 | Q1 |
| 2025 | 4.000 | Q2 |
JOURNAL OF CHROMATOGRAPHY A 最新收录文献
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1. Solvent-free synthesis of a magnetic covalent organic framework as a high-performance adsorbent for selective extraction and ultrasensitive determination of Bi(III), Hg(II), Ag(I), and Pb(II).
1. 无溶剂法合成磁性共价有机框架作为高性能吸附剂用于选择性萃取和超灵敏测定Bi(III)、Hg(II)、Ag(I)和Pb(II)PMID:日期:2026-09-29A novel urea-based magnetic covalent organic framework composite (FeO@U-COF) was synthesized via a simple, solvent-free mechanochemical method and introduced as a high-performance adsorbent for sample preparation. Comprehensive characterization confirmed a synergistic enhancement in the material's properties. The resulting nanocomposite exhibited a specific surface area (76.05 m g) significantly greater than its individual components, which is crucial for achieving high adsorption efficiency. The material was successfully applied in a magnetic solid-phase microextraction (MSPME) method for the selective preconcentration of trace Bi(III), Hg(II), Ag(I), and Pb(II) ions. The quantitative determination of the analytes was performed by inductively coupled plasma optical emission spectrometry. Following a systematic optimization of all critical parameters, the optimal conditions were established: an adsorbent amount of 1 mg, a sample volume of 10 mL, and elution with 300 µL of 2 mol l HCl. Notably, the method demonstrated exceptional robustness, operating effectively across a wide pH range (5-9), which advantageously eliminates the need for sample pH adjustment. Under optimal conditions, the method achieved very low limits of detection (0.03-300 ng l), wide linear dynamic ranges, satisfactory enhancement factors, and excellent precision, with intra-day relative standard deviations below 2.7%. The practical applicability and accuracy of the method were validated through the analysis of diverse real-world samples, including environmental waters and human plasma, yielding excellent relative recoveries (90-115%) and demonstrating its high tolerance to matrix interferences.
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2. Comparative contaminant and chemical profiling of commercial yerba mate infusions prepared as chimarrão and tereré from Southern Common Market countries.
PMID:日期:2026-09-29Yerba mate (Ilex paraguariensis A. St.-Hil.) is widely consumed in South America and contains diverse naturally occurring compounds, while potentially hazardous contaminants may be introduced during cultivation, processing, and commercialization. In this study, a direct immersion solid-phase microextraction coupled with single-quadrupole gas chromatography-mass spectrometry (DI-SPME/GC-MS) method was developed for targeted determination of selected phthalates and pesticides and untargeted chemical profiling of commercial yerba mate from Southern Common Market (MERCOSUL) countries (Paraguay, Argentina, Brazil, and Uruguay). Extraction conditions were evaluated using a factorial design, identifying agitation speed and salt addition as the most influential variables. The method showed satisfactory analytical performance, with R² > 0.99, recoveries of 84-104%, relative standard deviations (RSDs) below 8%, and limits of detection of 0.31-3.04 μg L⁻¹. Analysis of 48 commercial samples revealed frequent occurrence of phthalates and pesticides, with dibutyl phthalate (DBP) showing the highest detection frequency (67%). Untargeted GC-MS screening identified 476 and 673 compounds in tereré and chimarrão, respectively, with greater chemical diversity in the hot infusion. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) identified preparation temperature as the main factor associated with sample discrimination, while country of origin and drying conditions showed secondary effects. Naturally occurring compounds, polycyclic aromatic hydrocarbons (PAHs), and phthalate-related compounds were detected, suggesting contributions from smoke-drying and packaging-related contamination. These findings demonstrate the suitability of DI-SPME/GC-MS for contaminant monitoring and chemical profiling of yerba mate infusions.
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3. Visualized separation of exosomes in urine using carrier ampholyte-free free-flow isoelectric focusing electrophoresis.
3. 使用无载体两性电解质自由流等电聚焦电泳对尿液中外泌体进行可视化分离PMID:日期:2026-09-28Free flow electrophoresis (FFE) is a fully liquid-phase electrophoretic separation technique without supporting media, featuring mild separation conditions, continuous separation capability, and compatibility with multiple electrophoretic modes compared with other electrophoretic methods. In this work, a carrier ampholyte-free free-flow isoelectric focusing (CA-free FFIEF) device was constructed via anion/cation exchange membranes paired with strong acid/alkali electrode solutions (500 mmol·L⁻¹ phosphoric acid/1000 mmol·L⁻¹ sodium hydroxide). A stable linear pH gradient (4.07-9.35) was formed along the electric field direction in the separation chamber under an applied electric field of 300 V, enabling isoelectric focusing separation of exosomes. Based on the differences in particle size and surface charge of exosomes, a separation and preparation workflow for exosomes via CA-free FFIEF was established by optimizing electric field strength, buffer pH, flow rate and other parameters, achieving efficient separation of urinary exosomes. Carbon quantum dots (CQDs) were utilized to label exosomes to realize visualized separation throughout the process. Afterwards, metal oxide affinity chromatography (MOAC) with TiO microspheres was applied to purify FFIEF-fractionated exosomes to eliminate protein contaminants and enrich target vesicles. The CA-free FFIEF system separated two subtypes of urinary exosomes with distinct isoelectric points, with a total exosome recovery of 95.91%. After sequential FFIEF and TiO purification, the overall protein residual ratios in fraction 7 was as low as 1.77%. A total of 1802 proteins were identified in high-abundance fractions, among which 84.2% were exosome marker proteins recorded in the ExoCarta database. Distinct protein expression profiles were observed across different fractions, providing resources for screening differential biomarkers of urinary system diseases. This study offers a novel strategy for rapid and high-efficiency separation of urinary exosomes and lays a foundation for fundamental exosome research and translational clinical applications.
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4. DES-impregnated electrospun nanofiber microextraction for simultaneous determination of carvedilol and verapamil in human plasma by HPLC-fluorescence detection.
PMID:日期:2026-09-28The development of efficient and environmentally compatible sample preparation strategies for the determination of pharmaceuticals in complex biological matrices remains an important challenge in bioanalytical chemistry. Herein, a deep eutectic solvent (DES)-impregnated electrospun nanofiber microextraction (DES-ENF-ME) approach was developed for the simultaneous extraction of carvedilol and verapamil from human plasma prior to HPLC-fluorescence detection. A hydrophobic fatty-acid-based DES composed of nonanoic acid and dodecanoic acid (3:1, molar ratio) was selected and impregnated onto electrospun polyacrylonitrile nanofibers. In this configuration, the DES acts as the primary extraction phase, while the nanofibrous matrix provides a porous support for retaining and handling the liquid extraction phase. Sample pH, nanofiber amount, salt concentration, and extraction time were simultaneously optimized using central composite design and a desirability function. The method exhibited a linear response over the range of 1-1000 ng/mL for both analytes, with coefficients of determination of 0.9988 and 0.9942 for carvedilol and verapamil, respectively. The corresponding limits of detection were 0.396 and 0.342 ng/mL. The method showed satisfactory precision and relative recoveries for spiked plasma samples. Moreover, the DES-impregnated nanofibrous material was reusable for at least four consecutive extraction cycles. Overall, the proposed configuration combines the extraction capability of a hydrophobic DES with the practical handling advantages of an electrospun nanofibrous support, providing a simple, miniaturized, and solvent-efficient platform for plasma bioanalysis.
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5. Two-dimensional non-equilibrium model of gradient elution chromatography incorporating dual-mode gradients and core-shell particles.
5. 结合双模式梯度和核壳颗粒的梯度洗脱色谱二维非平衡模型PMID:日期:2026-09-28A two-dimensional non-equilibrium (lumped kinetic) model is developed for non-isothermal, dual-mode gradient elution chromatography in columns packed with core-shell particles. The model couples mobile- and stationary-phase mass balances, fluid- and solid-phase energy balances, and a solvent volume fraction transport equation with a competitive multicomponent Langmuir isotherm, yielding nonlinear, convection-dominated partial differential equations. Three retention models, linear solvent strength (LSS), quadratic solvent strength (QSS), and Neue-Kuss, are incorporated with van't Hoff temperature dependence, and a dual-mode gradient programs mobile-phase composition and column temperature as piecewise-linear profiles. Sample is injected through the inner cylindrical inlet zone, resolving radial dispersion explicitly. The equations are solved by a high-resolution finite volume scheme with the Koren flux limiter and a second-order total-variation-diminishing Runge-Kutta integrator. Parametric studies relate core-radius fraction, adsorption enthalpy, isotherm nonlinearity, transfer coefficients, Péclet numbers, and gradient timing to cut time, cycle time, productivity, and yield. The core-radius fraction is the dominant design variable: an intermediate value (ξ≈0.4) roughly halves analysis time while preserving resolution. QSS provides the fastest elution and highest productivity, whereas the Neue-Kuss model provides the broadest preservation of selectivity. The gradient start time is an effective control parameter for separation performance, whereas variations in the gradient end time have a comparatively limited effect. The results guide intensification of dual-mode gradient protocols in preparative high-performance liquid chromatography.
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6. Centrifugal mini-SPE cleanup for monitoring contaminants in food. Part II: Sorbents capacity, quantification, and comparison of lots.
6. 离心微型固相萃取净化用于食品中污染物监测。第二部分：吸附剂容量、定量及批次比较PMID:日期:2026-09-27The goal of this study was to investigate cleanup and analytical performance of three different lots of a centrifugal mini-cartridge solid-phase extraction (mini-SPE) product. The study assessed the cleanup of QECh*All extracts from five food matrix types (liquid infant formula, orange, grains, catfish, and kale), followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis of 47 pesticides and contaminants spanning a LogP range of -1 to 8. A second lab used low-pressure (LP)GC- high-resolution (HR)MS with an orbital ion trap (orbitrap) instrument for quantification of the same samples and a separate assessment of sorbents capacity in cleanup. Cleanup of 125 mg equivalent sample in 0.5 mL extract was found to provide >90% more removal of common matrix background components (e.g. fatty acids, cholesterol) than when using more concentrated extracts. Monitoring more than a dozen matrix markers in final extracts within 5 ppm mass accuracy demonstrated that the degree of cleanup generally exceeded the dilution factor, which is an interesting and useful empirical finding for analysts using one-step SPE for cleanup. In GC-MS/MS, an analytical quality control standard (triphenylphosphate) exhibited an RSD of 4.8% (n = 83), while the internal standard (chlorpyrifos-d) showed a recovery of 97 ± 5% (n = 45). Mean recoveries and RSDs for the targeted analytes were 98% and 8%, respectively, and matrix effects were within ±10% for nearly all analytes. No statistically consistent differences were observed among the three product lots, each containing 20 mg of anhydrous magnesium sulfate (MgSO₄) and 12 mg each of primary-secondary amine (PSA) and octadecylsilane (C18) sorbents. Degradation of labile pesticides, such as captan-d, folpet(-d), chlorothalonil, and ¹³C-p,p'-DDT, was substantially reduced compared to other methods, with only partial degradation occurring in kale extracts. QECh*All with centrifugal mini-SPE cleanup was also demonstrated to achieve high-throughput in an easy procedure that an individual analyst followed to prepare 75 final extracts in <3 h.
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7. High-capacity polymer-based weak cation exchanger for ion chromatography.
7. 用于离子色谱的高容量聚合物基弱阳离子交换剂PMID:日期:2026-09-27A novel polymer-based weak cation exchanger functionalized with dicarboxyl groups is described. The stationary phase was synthesized through radical-initiated copolymerization of maleic anhydride and glycidyl methacrylate onto porous poly(styrene-divinylbenzene) microspheres, followed by hydrolysis to yield carboxylate groups. The resultant cation exchanger enables simultaneous isocratic separation of alkali and alkaline earth metals (including NH₄⁺) using a single strong acid eluent. High column efficiencies were achieved (e.g., 31,900 and 35,800 plates per meter for Na⁺ and K⁺, respectively) and high capacity was obtained (4.80 mmol/column or 1.93 μmol/mm). The column also demonstrated good selectivity toward organic amines and other metal ions. The method was successfully applied to the determination of cations in tap water and beer matrices, with recoveries ranging from 92.7% to 109.4%, highlighting its potential for complex sample analysis.
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8. Increasing peak capacity in preparative liquid chromatography for pharmaceutical drug discovery using elevated temperature and column downsizing.
8. 利用升温和縮小柱径提高制备液相色谱在药物发现中的峰容量PMID:日期:2026-09-26Preparative reversed-phase liquid chromatography remains a cornerstone technology for rapid, scalable purification of small-molecule pharmaceutical compounds providing easy access to high-purity compounds for structure-activity relationship studies, lead optimization, and early developability assessments. While temperature control is rarely optimized in preparative workflows, this study demonstrates that integrating elevated column and solvent temperatures with column downsizing (to 19 mm ID) doubles peak capacity, maintains large sample loading (up to 200 mg), and reduces solvent consumption by more than half, all with robust separation quality. Key operational strategies include dynamic heating to prevent temperature gradients and maximize reproducibility. Together, these optimizations provide an efficient, scalable, and cost-effective approach to compound purification in pharmaceutical drug discovery.
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9. Deciphering complex charge heterogeneity in a glycosylated monoclonal antibody by integrated icIEF-MS and enzymatic digestion system.
9. 通过集成icIEF-MS和酶解系统解析糖基化单克隆抗体中复杂的电荷异质性PMID:日期:2026-09-26Charge heterogeneity is a critical quality attribute of therapeutic monoclonal antibodies. However, structurally characterizing charge variants resolved by imaged capillary isoelectric focusing (icIEF) remains challenging for antibodies with complex charge profiles. Here, we established an integrated enzymatic digestion and direct icIEF-MS workflow, enabling the elucidation of charge heterogeneity origins in a highly glycosylated monoclonal antibody that exhibits an unusually complex, multi-fingered icIEF profile. icIEF revealed eight well-resolved charge variants. icIEF-MS analysis identified that the basic variants were primarily attributed to incomplete C-terminal lysine clipping, whereas the major acidic variants originated predominantly from sialylated glycan species. Sequential treatment with carboxypeptidase B, PNGase F, neuraminidase, and IdeS facilitated the systematic assignment of the charge variants and indicated that the sialylated glycans responsible for the acidic peak cluster were mainly located in the Fab region. Following neuraminidase treatment, the multi-fingered acidic profile largely collapsed into a single main peak, confirming sialylation as the dominant source of charge heterogeneity. Residual low-level acidic variants with reduced pI but no obvious intact-mass differences were attributed to deamidation. Overall, this work demonstrates that combining direct icIEF-MS with orthogonal enzymatic digestion strategies enables a more confident elucidation of complex antibody charge heterogeneity. This workflow provides a practical framework for in-depth characterization and quality assessment of complex charge variants in biopharmaceutical.
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10. Impact of sulfonium versus ammonium groups in zwitterions on hydrophilic interaction chromatographic separation performance.
10. 锍基与铵基在两性离子中对亲水相互作用色谱分离性能的影响PMID:日期:2026-09-24The separation performance of zwitterionic hydrophilic interaction chromatography (HILIC) is highly dependent on the molecular structure of zwitterion. Notably, sulfonium cations possess distinct physicochemical properties compared to ammonium cations in conventional stationary phases. In this study, the impact of sulfonium‑ vs. ammonium‑based zwitterions on hydrophilic interaction chromatographic performance was systematically investigated using monolithic column technology. Both sulfonium and ammonium-based zwitterionic monoliths were prepared by copolymerizing 3-(methyl{2-[(2-methylacryloyl)oxy]ethyl}sulfaniumyl)propane-1-sulfonate (MMSPS) and N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine (SPE), respectively, with crosslinker N,N'-methylenebisacrylamide (MBA). The novel poly(MMSPS-co-MBA) monolith exhibited excellent column efficiency (∼100,000 plates/m), permeability, mechanical stability, and separation performance. Compared to the ammonium-based poly(SPE-co-MBA) monolith, the sulfonium-based monolith showed higher surface charge density, which resulted in prolonged retention of amine compounds and accelerated the elution of benzoic acid derivatives and phenolic analytes due to weaker hydrogen-bonding interactions, respectively. Moreover, the sulfonium-based monolith demonstrated superior performance in the enrichment of N-glycopeptide from a human immunoglobulin G tryptic digest, benefiting from its high hydrophilicity and enhanced electrostatic interaction. Overall, this study not only clarifies the significant influence of the cationic group on the chromatographic behavior of zwitterionic HILIC stationary phases, but also highlights the distinct hydrophilicity, electrostatic properties, and hydrogen-bonding characteristics of sulfonium-based zwitterion in comparison to ammonium-based counterparts.