离子交换色谱和混合模式色谱带电固定相的最新发展
Recent development in charged stationary phases for ion exchange chromatography and mixed-mode chromatography.
摘要(中文译文)
离子交换色谱（IEC）与混合模式色谱凭借其独特的离子交换或混合模式保留机制，在极性及离子性化合物的分离中具有高效性，适用范围涵盖无机离子、蛋白质、多肽、药物、核苷酸及有机酸等。作为色谱系统的核心组成部分，固定相直接决定分离选择性与柱效。因此，新型固定相的合理设计与开发始终是现代分离科学的研究焦点。面对日益复杂的样品基质，近年来的研究进展推动了高性能离子交换色谱固定相的发展，使其正从单一离子交换机制向复杂的多功能设计演变。为更好地阐明支配色谱行为的关键结构-性能关系，本综述全面概述了过去十年间用于制备现代固定相的基质演变及表面修饰策略（如点击化学、可控自由基聚合及超支化修饰）。此外，本综述最后对色谱填料当前面临的挑战及未来发展趋势进行了批判性讨论。
Abstract
Ion exchange chromatography (IEC) and mixed-mode chromatography are highly effective for the separation of polar and ionic compounds, including inorganic ions, proteins, peptides, pharmaceuticals, nucleotides, and organic acids, owing to their unique ion exchange or mixed-mode retention mechanisms. As the core component of chromatographic system, the stationary phase directly determines separation selectivity and column efficiency. Therefore, the rational design and development of novel stationary phases remain a focal in modern separation science. In response to the increasing complexity of sample matrices, recent advances have driven the evolution of high‑performance IEC stationary phases that are shifting from a single ion exchange mechanism toward sophisticated multifunctional designs. To better elucidate the critical structure-performance relationships governing the chromatographic behavior, this review provides a comprehensive overview of the evolution of substrates and surface modification strategies (e.g., click chemistry, controlled radical polymerization, and hyperbranched modification) employed in preparing of modern stationary phases over the past decade. Furthermore, this review concludes with a critical discussion of the current challenges and future development trends of chromatographic packing materials.
如何引用
AMA
Yufeng Shen, Bingcheng Yang, Zongying Li. Recent development in charged stationary phases for ion exchange chromatography and mixed-mode chromatography.. Journal of chromatography. A. 2026; doi:10.1016/j.chroma.2026.467291.
APA
Yufeng Shen, Bingcheng Yang, Zongying Li (2026). Recent development in charged stationary phases for ion exchange chromatography and mixed-mode chromatography.. Journal of chromatography. A. https://doi.org/10.1016/j.chroma.2026.467291
GB/T 7714
Yufeng Shen, Bingcheng Yang, Zongying Li. Recent development in charged stationary phases for ion exchange chromatography and mixed-mode chromatography.[J]. Journal of chromatography. A, 2026 doi:10.1016/j.chroma.2026.467291.
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