加拿大魁北克与虫媒疾病相关的蚊子病毒组的RNA宏基因组分析
RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.
摘要(中文译文)
蚊虫携带多样的病毒群落，包括具有医学重要性的虫媒病毒和昆虫特异性病毒，然而北温带地区蚊虫种群的病毒组特征仍研究不足。本研究采用宏基因组测序技术，对来自加拿大魁北克的存档蚊虫样本混合池进行分析，这些样本代表此前已鉴定为虫媒病毒携带者的多个蚊种。我们的分析鉴定出60种病毒，包括三种虫媒病毒、若干昆虫特异性病毒以及多种双重宿主非致病性病毒，揭示了这些蚊虫种群中存在的丰富病毒多样性。完整病毒基因组的系统发育分析显示了与来自不同地理区域的病毒之间的遗传关系。我们描述了一种新提出的分体库蚊 Tombus 样病毒（bipartite Culex tombus-like virus），并报告了35条病毒基因组序列的完整解析。这些结果凸显了宏基因组方法在全面表征蚊虫病毒组方面的实用性，并强调了其在加强魁北克及类似北方生态系统中新发虫媒病毒（包括西尼罗病毒）监测方面的潜力。
Abstract
Mosquitoes harbor diverse viral communities, including both medically important arboviruses and insect-specific viruses, yet the viromes of mosquito populations in northern temperate regions remains poorly characterized. In this study, we used metagenomic sequencing to analyse pools of archived mosquito samples from Québec, Canada representing multiple species previously identified as arbovirus carriers. Our analyses identified 60 viral species, including three arboviruses, several insect-specific viruses, and multiple dual-host non-pathogenic viruses, revealing the rich viral diversity present in these mosquito populations. Phylogenetic analysis of complete viral genomes demonstrated genetic relationships with viruses reported from diverse geographic regions. We describe, a newly proposed bipartite Culex tombus-like virus and report the complete resolution of thirty-five viral genomic sequences. These results highlight the utility of metagenomic approaches for comprehensive characterization of the mosquito virome and underscore their potential to enhance surveillance of emerging arboviruses, including West Nile virus, in Québec and similar northern ecosystems.
如何引用
AMA
Ines Levade, Benjamin Delisle, Éric Fournier, Christian Therrien. RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.. PloS one. 2026; doi:10.1371/journal.pone.0350663.
APA
Ines Levade, Benjamin Delisle, Éric Fournier, Christian Therrien (2026). RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.. PloS one. https://doi.org/10.1371/journal.pone.0350663
GB/T 7714
Ines Levade, Benjamin Delisle, Éric Fournier, Christian Therrien. RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.[J]. PloS one, 2026 doi:10.1371/journal.pone.0350663.
在 PubMed增强版 阅读全文
翻译全文 · AI 文献问答 · PDF 原文下载 · 引用导出