智利包涵体肝炎/心包积水综合征暴发中禽腺病毒的特性分析
Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile.
摘要(中文译文)
1996至1997年间，从智利影响肉鸡和肉种鸡的包涵体肝炎/心包积水综合征（IBH/HPS）现场暴发中获得的3株禽腺病毒（FAV）分离株（341、344和215），通过病毒中和试验（VNTs）和DNA片段限制性酶切分析进行了鉴定。此外，在实验感染鸡中研究了其中一株FAV分离株（FAV 341）的病理学特征。用分离株341和344针对属于12个FAV血清型的参考株及抗血清进行VNTs，结果显示与FAV血清4型参考株KR5具有密切的抗原关系。采用引物H3/H4进行聚合酶链反应，随后用HpaII酶切，对分离株341和215进行血清型鉴定。分离株341、215及参考株KR5（FAV4）的PCR产物长度和限制性酶切图谱完全一致。本研究结果证实所有3株分离株均为FAV4。用1000个平均组织感染剂量的分离株341肌肉接种20日龄无特定病原体鸡进行致病性试验，结果在接种后第6天有9%（2只鸡）死亡。两只鸡均表现出特征性的IBH/HPS大体和显微病变；其余鸡在接种后第10天剖杀时病变较轻。基于智利FAV分离株毒力的流行病学和实验数据以及分离株341的致病性结果，我们推测智利FAV毒株可能需要与其他病原（免疫抑制性病原）协同作用才能在现场引发IBH/HPS暴发。
Abstract
Three fowl adenovirus (FAV) isolates (341, 344, and 215) obtained during 1996-97 from field outbreaks of inclusion body hepatitis/hydropericardium syndrome (IBH/HPS) affecting broilers and broiler breeders in Chile were characterized by virus neutralization tests (VNTs) and restriction enzyme analysis of a DNA fragment. Furthermore, the pathologic characteristics of one of these FAV isolates (FAV 341) was studied in experimentally infected chickens. The VNTs conducted with isolates 341 and 344 against reference strains and antisera belonging to each of 12 FAV serotypes demonstrated a close antigenic relationship with strain KR5 of the FAV serotype 4. Polymerase chain reaction using the primers H3/H4 and subsequent HpaII digestion was used for serotype identification of isolates 341 and 215. The length of the PCR products and the restriction profiles of isolates 341, 215, and the reference strain KR5 (FAV4) were identical. The present results confirmed the classification of all three isolates as FAV4. The pathogenicity test with 1000 mean tissue infectious dose of isolate 341 inoculated intramuscularly in 20-day-old specific-pathogen-free chickens resulted in the death of 9% (two birds) six days postinoculation (PI). Both birds showed characteristic IBH/HPS gross and microscopic lesions; the remaining birds, sacrificed at day 10 PI, showed less severe lesions. On the basis of epidemiologic and experimental data of the virulence of Chilean FAV isolates, and the pathogenicity results with isolate 341, we speculate that Chilean FAV strains may require an association with other agents (immunosuppressive agents) to induce IBH/HPS outbreaks in the field.
如何引用
AMA
H Toro, C Prusas, R Raue, L Cerda, C Geisse, C González, et al. Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile.. Avian diseases.
APA
H Toro, C Prusas, R Raue, L Cerda, C Geisse, C González, et al Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile.. Avian diseases.
GB/T 7714
H Toro, C Prusas, R Raue, L Cerda, C Geisse, C González, et al. Characterization of fowl adenoviruses from outbreaks of inclusion body hepatitis/hydropericardium syndrome in Chile.[J]. Avian diseases
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