从明尼苏达州野鸭和羽毛中分离甲型流感病毒（2010-2011年）

摘要(中文译文)

我们研究了以羽毛作为补充方法检测野鸭种群中低致病性甲型流感病毒（IAVs）的可行性。2010年和2011年，在美国明尼苏达州的四个鸭子捕获点收集了地面上的羽毛。从捕获时采集的鸭羽毛和泄殖腔拭子中均分离出了IAV。尽管羽毛和泄殖腔拭子的病毒分离率在各次采集之间不一致，但羽毛样本的总体分离率最高。从羽毛中分离出的病毒也反映了在泄殖腔拭子分离株中观察到的亚型多样性，但产生了更多含有一种以上病毒的分离株。我们的研究表明，检测羽毛可能是一种替代性的非侵入性方法，用于回收病毒并估计亚型丰度和多样性。

Abstract

We investigated the feasibility of testing feathers as a complementary approach to detect low pathogenic influenza A viruses (IAVs) in wild duck populations. Feathers on the ground were collected at four duck capture sites during 2010 and 2011, in Minnesota, U. S. A. IAVs were isolated from both feathers and cloacal swabs sampled from ducks at the time of capture. Although virus isolation rates from feather and cloacal swabs were inconsistent between collections, the overall rate of isolation was greatest from the feather samples. Viruses isolated from feathers also reflected the subtype diversity observed in cloacal swab isolates but resulted in many more isolates that contained more than one virus. Our study suggests that testing feathers may represent an alternative noninvasive approach to recover viruses and estimate subtype abundance and diversity.

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